Autoaggregation Response of
            <i>Fusobacterium nucleatum</i>

Merritt, Justin; Niu, Guoqing; Okinaga, Toshinori; Qi, Felicia

doi:10.1128/aem.00916-09

Cited by 36 publications

(39 citation statements)

References 38 publications

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“…Previously, it has been shown that F. nucleatum 25586 coaggregates with a variety of Streptococcus spp. in saliva diluted in growth medium . In addition, F. nucleatum ATCC 23726 coaggregates strongly with S. gordonii DL1 in coaggregation buffer .…”

Section: Resultsmentioning

confidence: 93%

Transcriptional responses of Streptococcus gordonii and Fusobacterium nucleatum to coaggregation

Mutha

Mohammed

Krasnogor

et al. 2018

Molecular Oral Microbiology

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Cell‐cell interactions between genetically distinct bacteria, known as coaggregation, are important for the formation of mixed‐species biofilms such as dental plaque. Interactions lead to gene regulation in the partner organisms that may be critical for adaptation and survival in mixed‐species biofilms. Here, gene regulation responses to coaggregation between Streptococcus gordonii and Fusobacterium nucleatum were studied using dual RNA‐Seq. Initially, S. gordonii was shown to coaggregate strongly with F. nucleatum in buffer or human saliva. Using confocal laser scanning microscopy and transmission electron microscopy, cells of different species were shown to be evenly distributed throughout the coaggregate and were closely associated with one another. This distribution was confirmed by serial block face sectioning scanning electron microscopy, which provided high resolution three‐dimensional images of coaggregates. Cell‐cell sensing responses were analysed 30 minutes after inducing coaggregation in human saliva. By comparison with monocultures, 16 genes were regulated following coaggregation in F. nucleatum whereas 119 genes were regulated in S. gordonii. In both species, genes involved in amino acid and carbohydrate metabolism were strongly affected by coaggregation. In particular, one 8‐gene operon in F. nucleatum encoding sialic acid uptake and catabolism was up‐regulated 2‐ to 5‐fold following coaggregation. In S. gordonii, a gene cluster encoding functions for phosphotransferase system‐mediated uptake of lactose and galactose was down‐regulated up to 3‐fold in response to coaggregation. The genes identified in this study may play key roles in the development of mixed‐species communities and represent potential targets for approaches to control dental plaque accumulation.

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Section: Resultsmentioning

confidence: 93%

Transcriptional responses of Streptococcus gordonii and Fusobacterium nucleatum to coaggregation

Mutha

Mohammed

Krasnogor

et al. 2018

Molecular Oral Microbiology

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“…nucleatum , which can further support the proliferation of all the other species as F . nucleatum is better known as a bridging-species in the oral biofilm [30, 31]. This could explain the increased overall CFEs, and in particular P .…”

Section: Resultsmentioning

confidence: 99%

In Vitro Effect of Porphyromonas gingivalis Methionine Gamma Lyase on Biofilm Composition and Oral Inflammatory Response

et al. 2016

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Methanethiol (methyl mercaptan) is an important contributor to oral malodour and periodontal tissue destruction. Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum are key oral microbial species that produce methanethiol via methionine gamma lyase (mgl) activity. The aim of this study was to compare an mgl knockout strain of P. gingivalis with its wild type using a 10-species biofilm co-culture model with oral keratinocytes and its effect on biofilm composition and inflammatory cytokine production. A P. gingivalis mgl knockout strain was constructed using insertion mutagenesis from wild type W50 with gas chromatographic head space analysis confirming lack of methanethiol production. 10-species biofilms consisting of Streptococcus mitis, Streptococcus oralis, Streptococcus intermedius, Fusobacterium nucleatum ssp polymorphum, Fusobacterium nucleatum ssp vincentii, Veillonella dispar, Actinomyces naeslundii, Prevotella intermedia and Aggregatibacter actinomycetemcomitans with either the wild type or mutant P. gingivalis were grown on Thermanox cover slips and used to stimulate oral keratinocytes (OKF6-TERT2), under anaerobic conditions for 4 and 24 hours. Biofilms were analysed by quantitative PCR with SYBR Green for changes in microbial ecology. Keratinocyte culture supernatants were analysed using a multiplex bead immunoassay for cytokines. Significant population differences were observed between mutant and wild type biofilms; V. dispar proportions increased (p<0.001), whilst A. naeslundii (p<0.01) and Streptococcus spp. (p<0.05) decreased in mutant biofilms. Keratinocytes produced less IL-8, IL-6 and IL-1α when stimulated with the mutant biofilms compared to wild type. Lack of mgl in P. gingivalis has been shown to affect microbial ecology in vitro, giving rise to a markedly different biofilm composition, with a more pro-inflammatory cytokine response from the keratinocytes observed. A possible role for methanethiol in biofilm formation and cytokine response with subsequent effects on oral malodor and periodontitis is suggested.

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“…Collective evidence from these studies suggests that coaggregation and autoaggregation promote the highly specific integration and expansion of bacterial populations within biofilm communities (Kolenbrander, 2000; Merritt et al, 2009; Min & Rickard, 2009; Rickard et al, 2002; Walter et al, 2008). Here we show that all 30 showerhead biofilm isolates were able to coaggregate with atleast one other isolate (Table 3), and that 13 of these were able to autoaggregate.…”

Section: Discussionmentioning

confidence: 99%

Coaggregation occurs amongst bacteria within and between biofilms in domestic showerheads

et al. 2012

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Showerheads support the development multi-species biofilms that can be unsightly, produce malodor, and may harbor pathogens. The outer surface spray plates of many showerheads support visible biofilms that likely contain a mixture of bacteria from freshwater and potentially from human users. Coaggregation, a mechanism by which genetically distinct bacteria specifically recognize one another, may contribute to the retention and enrichment of different species within these biofilms. The aim of this work was to identify the bacterial composition of outer spray plate biofilms of three domestic shower heads and to determine the inter- and intra-biofilm coaggregation ability of each culturable isolate. The bacterial composition of the three biofilms was determined by using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) and by culturing on R2A medium. An average of 31 genera per biofilm were identified using bTEFAP and a total of 30 isolates were cultured. Even though the microbial diversity of each showerhead biofilm differed, every cultured isolate was able to coaggregate with at least one other isolate from the same or different showerhead biofilm. Promiscuous coaggregating isolates belonged to the genera Brevundimonas, Micrococcus, and Lysobacter. This work suggests that coaggregation may be a common feature of showerhead biofilms. Characterization of the mechanisms mediating coaggregation, and the inter-species interactions they facilitate, may allow for novel strategies to inhibit biofilm development.

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Autoaggregation Response of Fusobacterium nucleatum

Cited by 36 publications

References 38 publications

Transcriptional responses of Streptococcus gordonii and Fusobacterium nucleatum to coaggregation

Transcriptional responses of Streptococcus gordonii and Fusobacterium nucleatum to coaggregation

In Vitro Effect of Porphyromonas gingivalis Methionine Gamma Lyase on Biofilm Composition and Oral Inflammatory Response

Coaggregation occurs amongst bacteria within and between biofilms in domestic showerheads

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