Auto-Rosette Formation by Human Thymocytes and Lymphocytes

Sandilands, G. P.; Gray, Kathleen; Cooney, Anne; Browning, J.D.; Anderson, John R.

doi:10.1016/s0140-6736(74)93019-0

Cited by 40 publications

(19 citation statements)

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“…INTRODUCTION The discovery in the early 1970's that a very high proportion of human thymus-derived lymphocyte population formed spontaneous rosettes with sheep erythrocytes (SRBC)1 became one of the important early bases for the identification and isolation of human T cells (1). In the course of studies of the binding properties of erythrocytes of other species to human lymphocyte populations, it was noted that a small proportion of human lymphocytes also formed rosettes with human erythrocytes (2)(3)(4)(5). These lymphocytes were also identified as T cells.…”

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“…The supernate was gently removed and the pellet in the tubes was mixed using a rotary shaker at 180 cycles/min for 3 100 ,ug streptomycin/ml, 2 mM L-glutamine, 25 mM Hepes buffer, and 5% heat-inactivated autologous plasma (the complete medium). UT, ARFT, and NRFT were cultured overnight at 370C in complete medium in plastic petri dishes.…”

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“…The percentage of ARFT in human thymocytes is greater than in peripheral blood and exposure to thymic factor causes a decrease in ARFT (2)(3)(4)23). It was also found recently by Palacios et al (7) that ARFT bound peanut agglutinin and that ARFT were hydrocortisone sensitive.…”

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“…In studies in which neuraminidase-treated RBC are used to form rosettes, 30-50% of T cells form ARFT (8); in other studies in which RBC are not neuraminidase treated, the percentage of ARFT in peripheral blood is <10% (2)(3)(4)(5)23). However, Palacios et al (6,7), using the latter technique, observed that 30% of T cells formed autorosettes.…”

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“…For example, Charreire and Bach (26,27) reported that the frequency of ARFT was increased in New Zealand black, nude, and old mice. Sandilands et al (3) reported an increase in ARFT in patients with melanoma, and Palacios et al (6,7) mentioned that the frequency ofARFT was decreased in patients with systemic lupus erythematosus (6,7). We have recently observed that the anti-T cell antibodies found in patients with systemic lupus erythematosus are preferentially able to kill ARFT (data submitted for publication).…”

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Autologous rosette-forming T cells regulate responses of T cells. Phenotypic and functional analysis of suppressor cells generated from autologous rosette-forming T cells after autologous mixed lymphocyte reactions.

Kumagai¹,

Scher²,

Green³

1981

J. Clin. Invest.

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A B S T R A C T An average of 5-9% of human peripheral blood of T lymphocytes form rosettes with autologous erythrocytes (ARFT). This population responded only slightly against autologous and allogeneic non-T cells. In contrast, T cells that did not form rosettes with autologous erythrocytes (NRFT) proliferated to a greater degree in auto-and allogeneic mixed lymphocyte reactions (MLR) and also in reactions to trinitrophenyl (TNP) modified autologous non-T cells (TNP-auto-MLR) as compared with ARFT or unfractionated T cells. The ARFT populations could suppress the increased allogeneic (allo)MLR and TNPauto-MLR of NRFT when the ARFT were added to the NRFT at the beginning of the cultures.Fluorescence-activated cell-sorter (FACS) analysis of these freshly obtained T cell fractions using monoclonal antibodies to subpopulations of T cells did not demonstrate any selective gain or loss of T cell subsets in the ARFT and NRFT as compared with unfractionated T cells. But when each T cell fraction was cultured separately for a week in the presence of autologous non-T cells (auto-MLR) and the cells were again analyzed' by fluorescence-activated cell sorter, there was an increase in OKT8-positive cells (suppressor/cytotoxic subset) only in the ARFT fraction. The above findings strongly suggest that suppressor T cells are generated from the ARFT fraction during an auto-MLR, these may then regulate the responses of NRFT.

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