Auto-focusing and control of micro-vision system

Li-ping, 周丽平 Zhou; Zhi-jun, 孙志峻 Sun; Quan, 张泉 Zhang

doi:10.3788/ope.20132103.0807

Cited by 5 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Z.Q. Zhou et al [102] proposed an autofocus and control method that uses a combination of the lifting wavelet transform and the Sobel edge detection operator to form a focus evaluation function and the self-organizing algorithm to perform unsupervised training on the focused and defocused images.…”

Section: Cell Searching and Positioningmentioning

confidence: 99%

A Review of Automated Microinjection of Zebrafish Embryos

et al. 2018

View full text Add to dashboard Cite

Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. However, factors such as the small cell size, high cell deformation tendency, and transparent zebrafish embryo membrane make the microinjection process difficult. Furthermore, this process has strict, specific requirements, such as chorion softening, avoiding contacting the first polar body, and high-precision detection. Therefore, highly accurate control and detection platforms are critical for achieving the automated microinjection of zebrafish embryos. This article reviews the latest technologies and methods used in the automated microinjection of zebrafish embryos and provides a detailed description of the current developments and applications of robotic microinjection systems. The review covers key areas related to automated embryo injection, including cell searching and location, cell position and posture adjustment, microscopic visual servoing control, sensors, actuators, puncturing mechanisms, and microinjection.

show abstract

Section: Cell Searching and Positioningmentioning

confidence: 99%

A Review of Automated Microinjection of Zebrafish Embryos

et al. 2018

View full text Add to dashboard Cite

show abstract

“…It is time consuming and is not suitable for high speed auto focusing applications. Although numerous attempts and improvements were carried out [26][27][28][29][30], microscope auto focus still has a lot of room for improvementin areas where fast and precision focus are required. In addition to purely rely on software algorithm improvement, some hardware were added to speed up the process of reaching the best focus [31][32].…”

Section: Introductionmentioning

confidence: 99%

Study of Microscope Autofocus Based on Weighted Light Intensity

Lu¹,

Tu²,

Wu³

et al. 2021

AJPA

View full text Add to dashboard Cite

Microscopes auto focusing is critically important for a great spectrum of scientific and industrial applications.Finding the best focus position for variety of surfaces under measurement proved to be challenging. In order to provide a rapid and accurate method for microscope focusing positioning, a laser focus deviate system was employed for microscope focusing position. The relationship between focusing sensitivity and the surface under measurement was investigated. First, the laser focus deviate technique is introduced in light of characteristic parameters such as the diameter of the focused spot, the laser wavelength and the distance from the optimal focus. Then, the focusing displacement versus spot position curve fitting is performed for a smooth isotropic surface to directly locate the best focusing position. Following, it is analyzed that when applied to anisotropic and rough surfaces, the centroid of the image spot after reflection/scattering from the surface will deviate from its original light intensity distribution, thus deviate from the calculated optimal focus position and cause focusing errors. Finally, a criterion of weighted light intensity value Q is introduced to achieve fast focusing of anisotropic surfaces in combination with the laser focusing deviate technique. The experimental results show that for a 20× microscope objective system, the focusing accuracy of this focusing positioning method is 1.7 µm on smooth surfaces; and is at the micro meter level for rough surfaces. It basically meets the requirements for rapid, stable and reliable microscope auto focusing needs.

show abstract