Auto-Anti-Idiotype Production during the Response to Antigen

Thorbecke, G. J.; Siskind, Gregory W.

doi:10.1007/978-1-4684-4739-2_26

Cited by 2 publications

(1 citation statement)

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“…28], Recently, idiotope-antigen-induced B cell proliferation and antibody production in vitro have also been demonstrated [29]. However, auto-anti-idiotypic antibody production in mice bear ing hybridomas secreting monoclonal antibodies with a well defined specificity has not yet been described.…”

Section: Inhibition By Auto-anti-idiotypic Antibodymentioning

confidence: 99%

Studies on Immunity in Hybridoma-Bearing Mice A. Immune Response to Antigens

Yin¹,

Furusawa²,

Hirano³

et al. 1987

Int Arch Allergy Immunol

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When BALB/c mice, which had rejected the anti-dinitrophenyl (DNP) IgE-producing hybridoma B 53, were immunized with DNP proteins, they produced much less anti-DNP antibodies than control (normal) mice. The anti-DNP plaque-forming cell (PFC) number was much less when spleen cells from mice immunized with DNP proteins were treated with sera of mice which had rejected the hybridoma B 53 than the PFC number from the same spleen cells not treated by the sera. The sera of mice which had rejected the hybridoma B 53 contained an inhibitor which was adsorbed and eluted from an anti-mouse immunoglobulin column and also a mouse anti-DNP IgG2a column. The inhibition of PFC was hapten-reversible. In Western blotting the eluates from the anti-DNP IgG2a column reacted as well with the blotted anti-DNP IgE B 53 as an anti-idiotypic antibody to anti-DNP IgE B 53. These criteria establish that the inhibitor in the sera of the mice which had rejected the B 53 tumor was an anti-idiotypic antibody of the type which mimics the epitope (DNP) of the immunizing antigen.

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Section: Inhibition By Auto-anti-idiotypic Antibodymentioning

confidence: 99%