Authors’ reply to letter to the editor by G. Calmanovici

New Organometallic Technetium Complexes for Radiopharmaceutical Imaging

“…The highly lipophilic protein spread over a hydrophilic surface and could have potential in the diagnosis of acute respiratory disease syndrome. The spreading properties of the labelled and native surfactant protein B were in coincidence, making labelled surfactant protein B a potential diagnostic radiopharmaceutical [122].…”

Side-chain selective and covalent labelling of proteins with transition organometallic complexes. Perspectives in biology

“…Very efficient radio-labelling of recombinant single chain antibody fragments (scFvs) with the clinically useful radioisotope 99m Tc was achieved by specific complexation of the His-tag located at the N-terminus end of the proteins sequence with the aquaion [ 99m Tc(CO) 3 (H 2 O) 3 ] + generated in situ [69]. Direct radiolabelling of the surfactant protein B was achieved by reaction with the aquaion [ 99m Tc(CO) 3 (H 2 O) 3 ] + under non-reductive conditions and the labelled protein retained its biological activity in vitro [70].…”