Author response: Two FGFRL-Wnt circuits organize the planarian anteroposterior axis

Scimone, M. Lucila; Cote, Lauren E.; Rogers, Travis; Reddien, Peter W.

doi:10.7554/elife.12845.025

Cited by 3 publications

(1 citation statement)

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“…Common knowledge is that there are some creatures in nature that can achieve individual or partial regeneration, such as the gecko tail, earthworms, and the planarian. [13][14][15][16][17] Imitating the consumption of body energy of these living creatures for tissue regeneration, we can manufacture a series of products with disintegration, reorganization, deformation and regeneration, such as self-powered and disintegration-reorganizationregeneration power supply, and magnetic disintegration-reconstruction microrobot of the Big Hero 6.…”

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confidence: 99%

Design and Mechanism of a Self‐Powered and Disintegration–Reorganization–Regeneration Power Supply with Cold Resistance

et al. 2021

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in the future, and the power supply plays a fundamental role in deformable disintegration-reorganization robots. [1][2][3][4][5][6] From the perspective of technique, there are two basic approaches to solve the power supply problem of the disintegration-reorganization-deformation robot. One is to install an independent energy-storage system on each module of the disintegration-reorganization-deformation robot, which is the easiest approach to realize by directly applying high-capacity batteries or supercapacitors at present. [7][8][9] However, the defect of this method is that a disintegration-reorganization-deformation module with the failure of energy storage will lead to the failure of this module in operation, which may seriously affect the original system that needs it to play a role. The other is to make each module possess a self-powered power supply with the ability of disintegration, reorganization and deformation, which can solve the trouble of energy storage. [10][11][12] Therefore, the design and development of self-powered and disintegration-reorganization-regeneration power supply applying for the disintegration-reorganization-deformation robot can not only accelerate the construction and development of science and technology, but also promote the great revolutionary breakthroughs in the fields of transportation, construction, intelligent industry, etc.Up to now, power supplies designed based on the electrochemical reaction principle have had unavoidable defects, in that a complete redox reaction must be formed inside the power supply to operate normally, which makes it unable to be reconstructed and regenerated. Hence, the design and interpretation of this self-powered and disintegration-reorganization-regeneration power supply are generally considered to be almost insurmountable obstacles to haunt both experimenters and theorists. Herein, a self-powered and disintegration-reorganization-regeneration power supply with relatively stable discharge for 8.3 h is realized by the principle of ion-selective diffusion, which regenerates by radical polymerization. Additionally, the mechanism is investigated systematically by molecular dynamics simulation, and this power supply with a variety of self-powered and disintegration-reorganizationregeneration units can discharge continuously at freezing temperatures and variable temperature (0-25 °C). As a hypothetical model, a self-powered and deformable arch bridge with disintegration and reorganization is fabricated. In the future, this power supply is expected to be applied in prosthetic limbs, bionic skins, implantable power supplies, mobile phones, portable computers, wearable devices, etc. Moreover, with the improvement of the stability and discharge life, it could promote major revolutionary breakthroughs in the fields of intelligent industrial automation, smart buildings, intelligent transportation systems, intelligent power systems, etc.

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confidence: 99%

Design and Mechanism of a Self‐Powered and Disintegration–Reorganization–Regeneration Power Supply with Cold Resistance

et al. 2021

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DDX24 is required for muscle fiber organization and the suppression of wound-induced Wnt activity necessary for pole re-establishment during planarian regeneration

Sarkar

Dubey

Jahagirdar

et al. 2022

Developmental Biology

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DDX24, a D-E-A-D box RNA helicase, is required for muscle fiber organization and anterior pole specification essential for head regeneration in planarians

Palakodeti

Sarkar

Dubey

et al. 2021

Preprint

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Planarians have a remarkable ability to undergo whole-body regeneration. The timely establishment of polarity at the wound site followed by the specification of the organizing centers- the anterior pole and the posterior pole, are indispensable for successful regeneration. In planarians, polarity, pole, and positional-information determinants are predominantly expressed by muscles. The molecular toolkit that enables this functionality of planarian muscles however remains poorly understood. Here we report that SMED_DDX24, a D-E-A-D Box RNA helicase and the homolog of human DDX24, is critical for planarian head regeneration. DDX24 is enriched in muscles and its knockdown leads to defective muscle-fiber organization and failure to re-specify anterior pole/organizer. Overall, loss of DDX24 manifests into gross misregulation of many well-characterized positional-control genes and patterning-control genes, necessary for organogenesis and tissue positioning and tissue patterning. In addition, wound-induced Wnt signalling was also upregulated in ddx24 RNAi animals. Canonical WNT-βCATENIN signalling is known to suppress head identity throughout bilateria, including planarians. Modulating this Wnt activity by β-catenin-1 RNAi, the effector molecule of this pathway, partially rescues the ddx24 RNAi phenotype, implying that a high Wnt environment in ddx24 knockdown animals likely impedes their normal head regeneration. Furthermore, at a sub-cellular level, RNA helicases are known to regulate muscle mass and function by regulating their translational landscape. ddx24 knockdown leads to the downregulation of large subunit ribosomal RNA and the 80S ribosome peak, implying its role in ribosome biogenesis and thereby influencing the translational output. This aspect seems to be an evolutionarily conserved role of DDX24. In summary, our work demonstrates the role of a D-E-A-D box RNA helicase in whole-body regeneration through muscle fiber organization, and pole and positional-information re-specification, likely mediated through translation regulation.

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Author response: Two FGFRL-Wnt circuits organize the planarian anteroposterior axis

Cited by 3 publications

References 0 publications

Design and Mechanism of a Self‐Powered and Disintegration–Reorganization–Regeneration Power Supply with Cold Resistance

Design and Mechanism of a Self‐Powered and Disintegration–Reorganization–Regeneration Power Supply with Cold Resistance

DDX24 is required for muscle fiber organization and the suppression of wound-induced Wnt activity necessary for pole re-establishment during planarian regeneration

DDX24, a D-E-A-D box RNA helicase, is required for muscle fiber organization and anterior pole specification essential for head regeneration in planarians

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