Author response: Sparse recurrent excitatory connectivity in the microcircuit of the adult mouse and human cortex

Seeman, Stephanie C.; Campagnola, Luke; Davoudian, Pasha A; Hoggarth, Alex; Hage, Travis A.; Bosma-Moody, Alice; Baker, Christopher A.; Lee, Jung Hoon; Mihalaş, Ştefan; Teeter, Corinne; Ojemann, Jeffrey G.; Gwinn, Ryder P.; Silbergeld, Daniel L.; Cobbs, Charles; Phillips, John W.; Lein, Ed; Murphy, Gabe J.; Koch, Christof; Zeng, Hongkui; Jarsky, Tim

doi:10.7554/elife.37349.032

Cited by 5 publications

(4 citation statements)

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“…It is likely to decrease the probability of successful, high resolution recordings from a large number of neurons. In addition, the quality of the measured signals (i.e., the signal to-noise ratio of the recordings) will also deteriorate so that the detection of small PSPs (10-20 µV) is severely compromised (Seeman et al, 2018). Furthermore, the time for recording from an individual synaptic connections will be relatively short, i.e., the characterization of this connection limited because of the restricted overall total recording time for all possible synaptic connections.…”

Section: Discussionmentioning

confidence: 99%

“…To directly target specific neuronal subpopulations, paired recordings have been conducted in acute brain slices from transgenic animals where one specific or several populations of neurons are labeled by fluorescent groups (e.g., GFP, YFP, tdTomato etc.) as in transgenic, knockin animals or via viral infection (Pfeffer et al, 2013;Seeman et al, 2018). Paired recordings can be combined easily with other cutting-edge techniques, such as optogenetics, Ca 2+ imaging, activity-dependent immediate early gene expression and pseudorabies virus retrograde tracing etc.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, the paired recording approach has also been adopted to record from synaptically coupled neurons in the intact brain of anesthetized mice (Jouhanneau et al, 2015(Jouhanneau et al, , 2018. Paired recordings have also been used to investigate the functional and structural properties of synapses in surgically dissected human brain slices (Molnar et al, 2008;Testa-Silva et al, 2010Boldog et al, 2018;Seeman et al, 2018). For human tissue, paired recording in slices is still the only method of choice to study the functional neuronal microcircuits in preparations from human brains.…”

Section: Discussionmentioning

confidence: 99%

“…Several slicing and incubation solutions for adult and senescent animal and human brain tissue are available under http://www.brainslicemethods.com/ (Ting et al, 2014(Ting et al, , 2018a. Finally, the connection probability of different neuron types is highly variable (from 5 to 70%) depending on both the presynaptic axonal projection and the postsynaptic dendritic arborization (Thomson and Lamy, 2007;Lefort et al, 2009;Fino et al, 2013;Pfeffer et al, 2013;Jiang et al, 2015;Markram et al, 2015;Radnikow et al, 2015;Seeman et al, 2018;Jouhanneau and Poulet, 2019). Therefore, choosing the appropriate strategy, either a random patch or a "searching" protocol (Qi et al, 2015), is critical for the success of paired recordings.…”

Section: Electrophysiological Morphological And/or Molecular Characmentioning

confidence: 99%

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Unveiling the Synaptic Function and Structure Using Paired Recordings From Synaptically Coupled Neurons

Yang

Ding

et al. 2020

Front. Synaptic Neurosci.

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Synaptic transmission between neurons is the basic mechanism for information processing in cortical microcircuits. To date, paired recording from synaptically coupled neurons is the most widely used method which allows a detailed functional characterization of unitary synaptic transmission at the cellular and synaptic level in combination with a structural characterization of both pre-and postsynaptic neurons at the light and electron microscopic level. In this review, we will summarize the many applications of paired recordings to investigate synaptic function and structure. Paired recordings have been used to study the detailed electrophysiological and anatomical properties of synaptically coupled cell pairs within a synaptic microcircuit; this is critical in order to understand the connectivity rules and dynamic properties of synaptic transmission. Paired recordings can also be adopted for quantal analysis of an identified synaptic connection and to study the regulation of synaptic transmission by neuromodulators such as acetylcholine, the monoamines, neuropeptides, and adenosine etc. Taken together, paired recordings from synaptically coupled neurons will remain a very useful approach for a detailed characterization of synaptic transmission not only in the rodent brain but also that of other species including humans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Electrophysiological Morphological And/or Molecular Characmentioning

confidence: 99%

See 2 more Smart Citations

Unveiling the Synaptic Function and Structure Using Paired Recordings From Synaptically Coupled Neurons

Yang

Ding

et al. 2020

Front. Synaptic Neurosci.

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Adaptation supports short-term memory in a visual change detection task

Garrett

Groblewski

et al. 2020

Preprint

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The maintenance of short-term memories is critical for survival in a dynamically changing world. Previous studies suggest that this memory can be stored in the form of persistent neural activity or using a synaptic mechanism, such as with short-term plasticity. Here, we compare the predictions of these two mechanisms to neural and behavioral measurements in a visual change detection task. Mice were trained to respond to changes in a repeated sequence of natural images while neural activity was recorded using two-photon calcium imaging. We trained two different models to perform the same visual change detection task. Compared to a recurrent neural network (RNN), we find that a feedforward neural network with short-term synaptic depression (STPNet) is more consistent with the pattern of behavioral responses to image changes in mice and the observed adaptation in neural responses across repeated image presentations. These results suggest that simple neural adaptation mechanisms may serve as an important bottom-up memory signal in this task, which can be used by downstream areas in the decision-making process.

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Scale-free dynamics in animal groups and brain networks

Ribeiro

Chialvo

Plenz

2020

Preprint

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Collective phenomena fascinate by the emergence of order in systems composed of a myriad of small entities. They are ubiquitous in nature and can be found over a vast range of scales in physical and biological systems. Their key feature is the seemingly effortless emergence of adaptive collective behavior that cannot be trivially explained by the properties of the system’s individual components. This perspective focuses on recent insights into the similarities of correlations for two apparently disparate phenomena: flocking in animal groups and neuronal ensemble activity in the brain. We first will summarize findings on the spontaneous organization in bird flocks and macro-scale human brain activity utilizing correlation functions and insights from critical dynamics. We then will discuss recent experimental findings that apply these approaches to the collective response of neurons to visual and motor processing, i.e. to local perturbations of neuronal networks at the meso- and microscale. We show how scale-free correlation functions capture the collective organization of neuronal avalanches in evoked neuronal populations in nonhuman primates and between neurons during visual processing in rodents. These experimental findings suggest that the coherent collective neural activity observed at scales much larger than the length of the direct neuronal interactions is demonstrative of a phase transition. We discuss the experimental support for either discontinuous or continuous phase transitions. We conclude that at or near a phase-transition neuronal information can propagate in the brain with the same efficiency as proposed to occur in the collective adaptive response observed in some animal groups.

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Author response: Sparse recurrent excitatory connectivity in the microcircuit of the adult mouse and human cortex

Cited by 5 publications

References 0 publications

Unveiling the Synaptic Function and Structure Using Paired Recordings From Synaptically Coupled Neurons

Unveiling the Synaptic Function and Structure Using Paired Recordings From Synaptically Coupled Neurons

Adaptation supports short-term memory in a visual change detection task

Scale-free dynamics in animal groups and brain networks

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