Author response: Sequential conformational rearrangements in flavivirus membrane fusion

Chao, Luke H.; Klein, Daryl E.; Schmidt, Aaron; Peña, Jennifer M

doi:10.7554/elife.04389.017

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the mature virus particle, these form an outer shell of 90 E protein and 90 M protein homo-dimers, arranged with icosahedral symmetry (Mukhopadhyay et al, 2005). Receptor-mediated endocytosis and exposure to low pH triggers the E proteins to undergo a large, irreversible conformational change, and rearrangement into 60 trimers (Chao et al, 2014). This is followed by fusion of the viral and host cell membranes, and subsequent release of the capsid and RNA genome into the cell (Mukhopadhyay et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Pushing the Envelope: Dengue Viral Membrane Coaxed into Shape by Molecular Simulations

et al. 2016

View full text Add to dashboard Cite

Dengue virus is a flavivirus responsible for millions of infections per year. Its surface contains a phospholipid bilayer, within which are embedded the envelope (E) and membrane (M) proteins, arranged with icosahedral geometry. Exposure to low pH triggers the E proteins to undergo conformational changes, which precede fusion with the host cell membrane and release of the viral genome. The flavivirus membrane exhibits significant local curvature and deformation, as revealed by cryoelectron microscopy (cryo-EM), but its precise structure and interactions with envelope components remain unclear. We now report simulations of the dengue viral particle that refine its envelope structure in unprecedented detail. Our final models are morphologically consistent with cryo-EM data, and reveal the structural basis for membrane curvature. Electrostatic interactions increased envelope complex stability; this coupling has potential functional significance in the context of the viral fusion mechanism and infective states.

show abstract

Section: Introductionmentioning

confidence: 99%