Author response: Reporter-ChIP-nexus reveals strong contribution of the Drosophila initiator sequence to RNA polymerase pausing

Shao, Wanqing; Alcantara, Sergio G-M; Zeitlinger, Julia

doi:10.7554/elife.41461.030

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4D). Sequence analysis revealed that core promoter sequences, Initiator (Inr) (Purnell et al, 1994) and Downstream Promoter Element (DPE) (Burke and Kadonaga, 1996), for Pol II association exist in a HETRP repeat with the criteria described in previous research (Shao et al, 2019) (Fig. S6A).…”

Section: Rna Polymerase II Is Recruited To Mod(mdg4)-n Binding Sitessupporting

confidence: 63%

Mod(mdg4) variants repress telomeric retrotransposon HeT-A by blocking subtelomeric enhancers

Takeuchi

Yokoshi

Kondo

et al. 2022

Preprint

View full text Add to dashboard Cite

Telomeres in Drosophila are composed of sequential non-LTR retrotransposons: HeT-A, TART, and TAHRE. Although they are repressed by the piRNA pathway in the germline, how these retrotransposons are regulated in somatic cells is poorly understood. Here, we show that specific splice variants of Mod(mdg4) repress HeT-A by blocking subtelomeric enhancers in ovarian somatic cells. We found that the Mod(mdg4)-N variant represses HeT-A expression most efficiently among the variants. Mod(mdg4)-N mutant flies show elevated HeT-A expression and female sterility. Mod(mdg4)-N-binding subtelomeric sequences exhibit enhancer-blocking activity, and recruitment of RNA polymerase II (Pol II) on subtelomeres by Mod(mdg4)-N is essential for this enhancer-blocking. Moreover, Mod(mdg4)-N functions to form chromatin boundaries of higher-order chromatin conformation but this mechanism is independent of its Pol II recruitment activity at telomeres/subtelomeres. This study provides a link between enhancer-blocking and telomere regulation, and two different molecular mechanisms exhibited by an insulator protein to orchestrate precise gene expression.

show abstract

Section: Rna Polymerase II Is Recruited To Mod(mdg4)-n Binding Sitessupporting

confidence: 63%