Author response: Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins

Lee, Chih-Yung S.; Putnam, Andrea; Lu, Tu; He, Shuaixin; Ouyang, John Paul T.; Seydoux, Géraldine

doi:10.7554/elife.52896.sa2

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed periodicity of 3 nucleotides in phase with ribosomes ( Figure 4D), indicating that the synthesis of CSR-1 22G-RNAs occurs on mRNA templates engaged in translation. In contrast, the HRDE-1 loaded 22G-RNAs of P granule dependent piRNA targets (Table S1) did not show phasing with ribosomes as observed due to a lack of 3 nucleotide periodicity ( Figure 4C and D), in agreement with the fact that P granules are devoid of translating mRNAs (Lee et al, 2020;Schisa et al, 2001).…”

Section: Translating Mrnas Serve As the Template For 22g-rna Biogenesissupporting

confidence: 75%

Translation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis

Singh

Cornes

et al. 2020

Preprint

View full text Add to dashboard Cite

In the Caenorhabditis elegans germline, thousands of mRNAs are concomitantly expressed with antisense 22G-RNAs, which are loaded into the Argonaute CSR-1. Despite their essential functions for animal fertility and embryonic development, how CSR-1 22G-RNAs are produced remains unknown. Here, we show that CSR-1 slicer activity is primarily involved in triggering the synthesis of small RNAs on the coding sequences of germline mRNAs and post-transcriptionally regulates a fraction of targets. CSR-1-cleaved mRNAs prime the RNA-dependent RNA polymerase, EGO-1, to synthesize 22G-RNAs in phase with ribosome translation in the cytoplasm, in contrast to other 22G-RNAs mostly synthesized in germ granules. Moreover, codon optimality and efficient translation antagonize CSR-1 slicing and 22G-RNAs biogenesis. We propose that codon usage differences encoded into mRNA sequences might be a conserved strategy in eukaryotes to regulate small RNA biogenesis and Argonaute targeting.

show abstract

Section: Translating Mrnas Serve As the Template For 22g-rna Biogenesissupporting

confidence: 75%

Translation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis

Singh

Cornes

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos

Abbatemarco

Bondaz

Schwager

et al. 2021

Journal of Cell Science

View full text Add to dashboard Cite

When exposed to stressful conditions, eukaryotic cells respond by inducing the formation of cytoplasmic ribonucleoprotein complexes called stress granules. Here we use C. elegans to study two proteins that are important for stress granule assembly in human cells: PQN-59, the human UBAP2L ortholog, and GTBP-1, the human G3BP1/2 ortholog. Both proteins assemble into stress granules in the embryo and in the germline when C. elegans is exposed to stressful conditions. None of the two proteins is essential for the assembly of stress-induced granules, as shown by the single and combined depletions by RNAi, and neither pqn-59 nor gtbp-1 mutant embryos show higher sensitivity to stress than control embryos. We find that pqn-59 mutants display reduced progeny and a high percentage of embryonic lethality, phenotypes that are not dependent on stress exposure and that are not shared with gtbp-1 mutants. Our data indicate that, in contrast to human cells, PQN-59 and GTBP-1 are not required for stress granule formation but that PQN-59 is important for C. elegans development.

show abstract

Author response: Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins

Cited by 2 publications

References 0 publications

Translation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis

Translation and codon usage regulate Argonaute slicer activity to trigger small RNA biogenesis

PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos

Contact Info

Product

Resources

About