Author response: Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris

Wilkerson, Brent A.; Zebroski, Heather L; Finkbeiner, Connor; Chitsazan, Alex; Beach, Kathy; Sen, Nilasha; Zhang, Renee C; Bermingham‐McDonogh, Olivia

doi:10.7554/elife.60108.sa2

2021

DOI: 10.7554/elife.60108.sa2

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Author response: Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris

Brent A. Wilkerson

Heather L Zebroski

Connor Finkbeiner

et al.

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Defining developmental trajectories of prosensory cells in human inner ear organoids at single-cell resolution

Ueda,

Nakamura,

Nie

et al. 2023

Development

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The inner ear sensory epithelia contain mechanosensitive hair cells and supporting cells. Both cell types arise from SOX2-expressing prosensory cells, but the mechanisms underlying the diversification of these cell lineages remain unclear. To determine the transcriptional trajectory of prosensory cells, we established a SOX2-2A-ntdTomato human embryonic stem cell line using CRISPR/Cas9, and performed single-cell RNA-sequencing analyses with SOX2-positive cells isolated from inner ear organoids at various time points between differentiation days 20 and 60. Our pseudotime analysis suggests that vestibular type II hair cells arise primarily from supporting cells, rather than bi-fated prosensory cells in organoids. Moreover, ion channel- and ion-transporter-related gene sets were enriched in supporting cells versus prosensory cells, whereas Wnt signaling-related gene sets were enriched in hair cells versus supporting cells. These findings provide valuable insights into how prosensory cells give rise to hair cells and supporting cells during human inner ear development, and may provide a clue to promote hair cell regeneration from resident supporting cells in individuals with hearing loss or balance disorders.

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Defining developmental trajectories of prosensory cells in human inner ear organoids at single-cell resolution

Ueda,

Nakamura,

Nie

et al. 2023

Development

View full text Add to dashboard Cite

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Author response: Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris

Cited by 1 publication

References 0 publications

Defining developmental trajectories of prosensory cells in human inner ear organoids at single-cell resolution

Defining developmental trajectories of prosensory cells in human inner ear organoids at single-cell resolution

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