Author response: Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies

Vogan, Jacob M.; Zhang, Xiaozhu; Youmans, Daniel T.; Regalado, Samuel G.; Johnson, Joshua Z; Hockemeyer, Dirk; Collins, Kathleen

doi:10.7554/elife.18221.018

2016

DOI: 10.7554/elife.18221.018

|View full text |Cite

Author response: Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies

Jacob M. Vogan

Xiaozhu Zhang

Daniel T. Youmans

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2020

Publication Types

Select...

Preprint1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Single-molecule imaging of telomerase RNA reveals a Recruitment – Retention model for telomere elongation

Laprade

Querido

Smith³

et al. 2020

Preprint

View full text Add to dashboard Cite

Extension of telomeres is a critical step in the immortalization of cancer cells. This complex reaction requires proper spatio-temporal coordination of telomerase and telomeres, and remains poorly understood at the cellular level. To understand how cancer cells execute this process, we combined CRISPR genome editing and MS2 RNA-tagging to image single-molecules of telomerase RNA (hTR). Real-time dynamics and photoactivation experiments of hTR in Cajal bodies (CBs) reveal that hTERT controls the exit of hTR from CBs. Single-molecule tracking of hTR at telomeres shows that TPP1-mediated recruitment results in short telomere-telomerase scanning interactions, then base-pairing between hTR and telomere ssDNA promotes long interactions required for stable telomerase retention. Interestingly, POT1 OB-fold mutations that result in abnormally long telomeres in cancers act by enhancing this retention step. In summary, single-molecule imaging unveils the life-cycle of telomerase RNA and provides a framework to understand how cancer-associated mutations mechanistically drive defects in telomere homeostasis.

show abstract

Single-molecule imaging of telomerase RNA reveals a Recruitment – Retention model for telomere elongation

Laprade

Querido

Smith³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Author response: Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies

Cited by 1 publication

References 0 publications

Single-molecule imaging of telomerase RNA reveals a Recruitment – Retention model for telomere elongation

Single-molecule imaging of telomerase RNA reveals a Recruitment – Retention model for telomere elongation

Contact Info

Product

Resources

About