Author Correction: Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes

Rheaume, Bruce A.; Jereen, Amyeo; Bolisetty, Mohan; Sajid, Muhammad Sohail; Yang, Yue; Renna, Kathleen; Sun, Lili; Robson, Paul; Trakhtenberg, Ephraim F.

doi:10.1038/s41467-018-05792-3

Cited by 15 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Single cell gene expression studies enable profiling of transcriptional regulation during complex biological processes and within highly heterogeneous cell populations. These studies allow the discovery of genes that identify certain subtypes of cells, or that mark an intermediate status during a biological process (Rheaume et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Dissecting the initiation of female meiosis in the mouse at single-cell resolution

Wang

Zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

ABSTRACTGerm cell meiosis is one of the most finely orchestrated events during gametogenesis with distinct developmental patterns in males and females. However, in mammals, the molecular mechanisms involved in this process remain not well known. Here, we report detailed transcriptome analyses of cell populations present in the mouse female gonadal ridges (E11.5) and the embryonic ovaries from E12.5 to E14.5 using single cell RNA sequencing (scRNA seq). These periods correspond with the initiation and progression of meiosis throughout the first stage of prophase I. We identified 13 transcriptionally distinct cell populations and 7 transcriptionally distinct germ cell subclusters that correspond to mitotic (3 clusters) and meiotic (4 clusters) germ cells. By comparing the signature gene expression pattern of 4 meiotic germ cell clusters, we found that the 4 cell clusters correspond to different cell status en route to meiosis progression, and therefore, our research here characterized detailed transcriptome dynamics during meiotic prophase I. Reconstructing the progression of meiosis along pseudotime, we identified several new genes and molecular pathways with potential critical roles in the mitosis/meiosis transition and early meiotic progression. Last, the heterogeneity within somatic cell populations was also discussed and different cellular states were identified. Our scRNA seq analysis here represents a new important resource for deciphering the molecular pathways driving meiosis initiation and progression in female germ cells and ovarian somatic cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Dissecting the initiation of female meiosis in the mouse at single-cell resolution

Wang

Zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Decades of studies show that RGCs, ACs, and BCs can be divided into subtypes, each possessing characteristic anatomy, visual response, and molecular expression profile (Gollisch and Meister, 2010 ; Masland, 2012 ; Sanes and Masland, 2015 ). Many of the ~30 RGC, ~130 AC and ~13 BC types can be marked with type-specific antibodies and a full list of such markers is within sight due to an increasingly complete molecular taxonomy of the retina (Wässle et al, 2009 ; Macosko et al, 2015 ; Shekhar et al, 2016 ; Rheaume et al, 2018 ; Peng et al, 2019 ; Tran et al, 2019 ). Furthermore, many marker genes have been targeted by transgenic approaches to create an increasingly complete encyclopedia of cell-type-specific mouse lines housed at commercial repositories.…”

Section: The Retinamentioning

confidence: 99%

New Optical Tools to Study Neural Circuit Assembly in the Retina

Olguin

Rochon

Krishnaswamy

2020

Front. Neural Circuits

View full text Add to dashboard Cite

During development, neurons navigate a tangled thicket of thousands of axons and dendrites to synapse with just a few specific targets. This phenomenon termed wiring specificity, is critical to the assembly of neural circuits and the way neurons manage this feat is only now becoming clear. Recent studies in the mouse retina are shedding new insight into this process. They show that specific wiring arises through a series of stages that include: directed axonal and dendritic growth, the formation of neuropil layers, positioning of such layers, and matching of co-laminar synaptic partners. Each stage appears to be directed by a distinct family of recognition molecules, suggesting that the combinatorial expression of such family members might act as a blueprint for retinal connectivity. By reviewing the evidence in support of each stage, and by considering their underlying molecular mechanisms, we attempt to synthesize these results into a wiring model which generates testable predictions for future studies. Finally, we conclude by highlighting new optical methods that could be used to address such predictions and gain further insight into this fundamental process.

show abstract

“…Based on morphology, functional properties, presynaptic partners and central projection patterns, approximately 30 different RGC subtypes have been characterised in the mouse retina [1,2,3]. Recent single cell RNA-seq analyses of RGCs have placed the potential number of distinct RGC subtypes at around 40 [4]. Differentiation of nascent RGCs into identifiable RGC subtypes occurs postnatally and is regulated by differential transcription factor expression.…”

Section: Specification Of Rgc Subtype Identitymentioning

confidence: 99%

“…Based on single cell RNA-seq transcriptome profiling, most subtypes of RGCs appear to be present proportionally in both eyes, although a few RGC subtypes predominate in one eye compared to the other [4]. Axonal tracing experiments also have demonstrated that ipsilaterally projecting RGCs are composed of more than one subclass of RGCs [61].…”

Section: Specification Of Rgc Subtype Identitymentioning

confidence: 99%

“…The vertebrate retina is composed of six major neuronal cells types: the photoreceptors (rods and cones), located in the outer nuclear layer, that respond to light, and the retinal interneurons (amacrine, bipolar and horizontal cells), located predominately in the inner nuclear layer that modify and relay the visual information from the photoreceptors to the retinal ganglion cells (RGCs) located mainly in the RGC layer at the inner surface of the retina (Figure 1A). RGCs, of which there are more than 30 distinct subtypes [1,2,3,4], are the only cells in the retina that extend axons out of the eye to make connections with visual targets in the brain. These connections are formed during development by the guidance of RGC axons along a relatively long, highly stereotypical pathway by cues arrayed within and around the forming optic pathway.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Wiring the Binocular Visual Pathways

Murcia‐Belmonte

Erskine

2019

IJMS

View full text Add to dashboard Cite

Retinal ganglion cells (RGCs) extend axons out of the retina to transmit visual information to the brain. These connections are established during development through the navigation of RGC axons along a relatively long, stereotypical pathway. RGC axons exit the eye at the optic disc and extend along the optic nerves to the ventral midline of the brain, where the two nerves meet to form the optic chiasm. In animals with binocular vision, the axons face a choice at the optic chiasm—to cross the midline and project to targets on the contralateral side of the brain, or avoid crossing the midline and project to ipsilateral brain targets. Ipsilaterally and contralaterally projecting RGCs originate in disparate regions of the retina that relate to the extent of binocular overlap in the visual field. In humans virtually all RGC axons originating in temporal retina project ipsilaterally, whereas in mice, ipsilaterally projecting RGCs are confined to the peripheral ventrotemporal retina. This review will discuss recent advances in our understanding of the mechanisms regulating specification of ipsilateral versus contralateral RGCs, and the differential guidance of their axons at the optic chiasm. Recent insights into the establishment of congruent topographic maps in both brain hemispheres also will be discussed.

show abstract

Author Correction: Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes

Cited by 15 publications

References 0 publications

Dissecting the initiation of female meiosis in the mouse at single-cell resolution

Dissecting the initiation of female meiosis in the mouse at single-cell resolution

New Optical Tools to Study Neural Circuit Assembly in the Retina

Wiring the Binocular Visual Pathways

Contact Info

Product

Resources

About