Author Correction: Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence

Rolland, Tyler J.; Peterson, Timothy E.; Singh, Raman Deep; Rizzo, Skylar A; Boroumand, Soulmaz; Shi, Ao; Witt, Tyra A.; Nagel, Mary; Kisby, Cassandra K.; Park, Sungjo; Rowe, Lois A.; Paradise, Christopher R.; Becher, Laura; Paradise, Brooke D.; Stalboerger, Paul G.; Trabuco, Emanuel C.; Behfar, Atta

doi:10.1038/s41536-022-00260-5

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“…Each lot of material undergoes quality control testing to ensure the final product and vesicles contained within conforms to the accepted definitions of EVs and specifically exosomes. For further characterization of the vesicles in the product, surface markers indicative of exosomal origin including CD63, CD9, CD41, and flotillin are confirmed (Rolland et al, 2022; Shi et al, 2021). In this manuscript, PEP will be referenced to as an exosome product.…”

Section: Methodsmentioning

confidence: 99%

Dose–response analysis after administration of a human platelet‐derived exosome product on neurite outgrowth in vitro

Saffari

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Pulos

et al. 2023

Biotech & Bioengineering

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Modulating the nerve's local microenvironment using exosomes is proposed to enhance nerve regeneration. This study aimed to determine the optimal dose of purified exosome product (PEP) required to exert maximal neurite extension. An in vitro dorsal root ganglion (DRG) neurite outgrowth assay was used to evaluate the effect of treatment with (i) 5% PEP, (ii) 10% PEP, (iii) 15% PEP, or (iv) 20% PEP on neurite extension (N = 9/group), compared to untreated controls. After 72 h, neurite extension was measured to quantify nerve regeneration. Live cell imaging was used to visualize neurite outgrowth during incubation. Treatment with 5% PEP resulted in the longest neurite extension and was superior to the untreated DRG (p = 0.003). Treatment with 10% PEP, 15% PEP, and 20% PEP was found to be comparable to controls (p = 0.12, p = 0.06, and p = 0.41, respectively) and each other. Live cell imaging suggested that PEP migrated towards the DRG neural regeneration site, compared to the persistent homogenous distribution of PEP in culture media alone. 5% PEP was found to be the optimal concentration for nerve regeneration based on this in vitro dose–response analysis.

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Section: Methodsmentioning

confidence: 99%