Author Correction: Effect of two (short-term) storage methods on load to failure testing of murine bone tissue

Tiefenboeck, Thomas M.; Payr, Stephan; Bajenov, Olga; Koch, Thomas; Komjati, Micha; Sarahrudi, Kambiz

doi:10.1038/s41598-020-66764-6

Cited by 1 publication

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“…36 Freezing in sterile phosphate-buffered saline (PBS) is a less deleterious form of storage for samples destined for mechanical testing. 37 Sets to undergo other assessments (e.g., histological staining) may be fixed, usually using formalin or organic solvents such as alcohols or acetone. Before scanning, bones are cleaned of surrounding tissue and are generally placed in a holder filled with a medium such as saline or ethanol and wrapped in low-density foam or gauze to limit desiccation and motion artifact.…”

Section: Sample Preparationmentioning

confidence: 99%

Methodology, selection, and integration of fracture healing assessments in mice

Knox

McGuire

Natoli

et al. 2021

Journal Orthopaedic Research

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Long bone fractures are one of the most common and costly medical conditions encountered after trauma. Characterization of the biology of fracture healing and development of potential medical interventions generally involves animal models of fracture healing using varying genetic or treatment groups, then analyzing relative repair success via the synthesis of diverse assessment methodologies. Murine models are some of the most widely used given their low cost, wide variety of genetic variants, and rapid breeding and maturation. This review addresses key concerns regarding fracture repair investigations in mice and may serve as a guide in conducting and interpreting such studies. Specifically, this review details the procedures, highlights relevant parameters, and discusses special considerations for the selection and integration of the major modalities used for quantifying fracture repair in such studies, including X-ray, microcomputed tomography, histomorphometric, biomechanical, gene expression and biomarker analyses.

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Section: Sample Preparationmentioning

confidence: 99%