Author Correction: Complete biosynthesis of cannabinoids and their unnatural analogues in yeast

Luo, Xiaozhou; Reiter, Michael; d’Espaux, Leo; Wong, J.Y.C.; Denby, Charles M.; Lechner, Anna; Zhang, Yunfeng; Grzybowski, Adrian T.; Harth, Simon; Lin, Wan-Ying; Lee, Hyunsu; Yu, Changhua; Shin, John H; Deng, Kai; Benites, Veronica T.; Wang, George; Baidoo, Edward E. K.; Chen, Yan; Dev, Ishaan; Kim, Young-Mo; Keasling, Jay D.

doi:10.1038/s41586-020-2109-z

Cited by 3 publications

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“…Finally, these compounds undergo decarboxylation to produce biologically active compounds THC, CBD, and CBC, which are the main cannabinoids placed in Cannabis spp. [2,8,34,51].…”

Section: Cannabinoidsmentioning

confidence: 99%

Fermentation Strategies for Production of Pharmaceutical Terpenoids in Engineered Yeast

2021

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Terpenoids, also known as isoprenoids, are a broad and diverse class of plant natural products with significant industrial and pharmaceutical importance. Many of these natural products have antitumor, anti-inflammatory, antibacterial, antiviral, and antimalarial effects, support transdermal absorption, prevent and treat cardiovascular diseases, and have hypoglycemic activities. Production of these compounds are generally carried out through extraction from their natural sources or chemical synthesis. However, these processes are generally unsustainable, produce low yield, and result in wasting of substantial resources, most of them limited. Microbial production of terpenoids provides a sustainable and environment-friendly alternative. In recent years, the yeast Saccharomyces cerevisiae has become a suitable cell factory for industrial terpenoid biosynthesis due to developments in omics studies (genomics, transcriptomics, metabolomics, proteomics), and mathematical modeling. Besides that, fermentation development has a significant importance on achieving high titer, yield, and productivity (TYP) of these compounds. Up to now, there have been many studies and reviews reporting metabolic strategies for terpene biosynthesis. However, fermentation strategies have not been yet comprehensively discussed in the literature. This review summarizes recent studies of recombinant production of pharmaceutically important terpenoids by engineered yeast, S. cerevisiae, with special focus on fermentation strategies to increase TYP in order to meet industrial demands to feed the pharmaceutical market. Factors affecting recombinant terpenoids production are reviewed (strain design and fermentation parameters) and types of fermentation process (batch, fed-batch, and continuous) are discussed.

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“…Finally, these compounds undergo decarboxylation to produce biologically active compounds THC, CBD, and CBC, which are the main cannabinoids placed in Cannabis spp. [2,8,34,51].…”

Section: Cannabinoidsmentioning

confidence: 99%

Fermentation Strategies for Production of Pharmaceutical Terpenoids in Engineered Yeast

2021

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Designing artificial pathways for improving chemical production

Tan

Hou

et al. 2023

Biotechnology Advances

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Evaluating the Metabolomic Profile and Anti-Pathogenic Properties of Cannabis Species

Monyela,

Kayoka,

Ngezimana

et al. 2024

Metabolites

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The Cannabis species is one of the potent ancient medicinal plants acclaimed for its medicinal properties and recreational purposes. The plant parts are used and exploited all over the world for several agricultural and industrial applications. For many years Cannabis spp. has proven to present a highly diverse metabolomic profile with a pool of bioactive metabolites used for numerous pharmacological purposes ranging from anti-inflammatory to antimicrobial. Cannabis sativa has since been an extensive subject of investigation, monopolizing the research. Hence, there are fewer studies with a comprehensive understanding of the composition of bioactive metabolites grown in different environmental conditions, especially C. indica and a few other Cannabis strains. These pharmacological properties are mostly attributed to a few phytocannabinoids and some phytochemicals such as terpenoids or essential oils which have been tested for antimicrobial properties. Many other discovered compounds are yet to be tested for antimicrobial properties. These phytochemicals have a series of useful properties including anti-insecticidal, anti-acaricidal, anti-nematicidal, anti-bacterial, anti-fungal, and anti-viral properties. Research studies have reported excellent antibacterial activity against Gram-positive and Gram-negative multidrug-resistant bacteria as well as methicillin-resistant Staphylococcus aureus (MRSA). Although there has been an extensive investigation on the antimicrobial properties of Cannabis, the antimicrobial properties of Cannabis on phytopathogens and aquatic animal pathogens, mostly those affecting fish, remain under-researched. Therefore, the current review intends to investigate the existing body of research on metabolomic profile and anti-microbial properties whilst trying to expand the scope of the properties of the Cannabis plant to benefit the health of other animal species and plant crops, particularly in agriculture.

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Author Correction: Complete biosynthesis of cannabinoids and their unnatural analogues in yeast

Cited by 3 publications

References 0 publications

Fermentation Strategies for Production of Pharmaceutical Terpenoids in Engineered Yeast

Fermentation Strategies for Production of Pharmaceutical Terpenoids in Engineered Yeast

Designing artificial pathways for improving chemical production

Evaluating the Metabolomic Profile and Anti-Pathogenic Properties of Cannabis Species

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