Author Correction: Combining CRISPRi and metabolomics for functional annotation of compound libraries

Connecting metabolome and phenotype: recent advances in functional metabolomics tools for the identification of bioactive natural products

“…29,30 To this end, Anglada-Girotto et al combined gene knockdowns with metabolic proling to develop a platform for functionally annotating the mode of action of small molecules including a set of antimicrobial and anticancer drugs against Escherichia coli, Mycobacterium smegmatis, and a human lung cancer cell line. 15 They produced a library of metabolic signatures by silencing a several hundred genes involved in key essential biological processes via CRISPRi. Subsequently, they proled the drug-induced metabolic changes of a range of small metabolites mainly human drugs.…”

“…Other experiments, such as (one-dimensional) 13 C-NMR, 15 N-NMR or 19 F-NMR have to be performed offline, as they are time-consuming. Further promising tools for either structural elucidation or affinity proling of, e.g., small molecules to proteins comprise a large variety of multi-dimensional methods such as TOCSY, 131 COSY, 132 NOESY/ROESY, 133 and HMBC.…”

“…1, right side). [13][14][15] The denition of "functional metabolomics" is in some cases vaguely dened, as many workows described as functional metabolomics strategies are nearly identical to regular non-targeted metabolomics studies where typically two or more groups of treatments or disease stages are compared (Fig. 2A).…”