Author Correction: Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

Ou, Xiuyuan; Liu, Yan; Lei, Xiaobo; Li, Pei; Mi, Dan; Ren, Lili; Guo, Li; Guo, Ruixuan; Chen, Ting; Hu, Jiaxin; Zou, Xiang; Mu, Zhixia; Chen, Xing; Chen, Jieyong; Hu, Keping; Jin, Qi; Wang, Jianwei; Qian, Zhaohui

doi:10.1038/s41467-021-22614-1

Cited by 31 publications

(22 citation statements)

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“…The clinical sign of respiratory tract was confirmed in rhesus macaques, golden hamster, domestic cats, tiger, lion, mink, and ferret; Viral transmission was observed in rhesus macaques, golden hamster, domestic cats, lion, tiger, mink, ferret, and Egyptian fruit bat 12–23,25–29,31–33,50 . For cell lines, SARS‐CoV‐2 can infect and replicate in human Caco2, Calu3, NHBE, and lung epithelial line A549 25,51–56 . Meanwhile, SARS‐CoV‐2 can replicate in rhesus monkey LLCMK2, rabbit RK‐13, cat CRFK, and pig ST, and PK‐15 cells lines 25,57 .…”

Section: Resultsmentioning

confidence: 94%

“…[12][13][14][15][16][17][18][19][20][21][22][23][25][26][27][28][29][31][32][33]50 For cell lines, SARS-CoV-2 can infect and replicate in human Caco2, Calu3, NHBE, and lung epithelial line A549. 25,[51][52][53][54][55][56] Meanwhile, SARS-CoV-2 can replicate in rhesus monkey LLCMK2, rabbit RK-13, cat CRFK, and pig ST, and PK-15 cells lines. 25,57 Overall, the above reports revealed that the SARS-CoV-2 cell entry, or at the least, the recognition of ACE2 to SARS-CoV-2 has widely existed in livestock and wide animals.…”

Section: Summary Of Animals and Cells Affected By Sars-cov-2mentioning

confidence: 99%

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Comparative analyses of ACE2 and TMPRSS2 gene: Implications for the risk to which vertebrate animals are susceptible to SARS‐CoV‐2

Huang

Jiang

Yan

2021

Journal of Medical Virology

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Along with the control and prevention of coronavirus disease 2019 transmission, infected animals might have potential to carry the virus to spark new outbreaks. However, very few studies explore the susceptibility of animals to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Viral attachment as a crucial step for cross‐species infection requires angiotensin‐converting enzyme 2 (ACE2) as a receptor and depends on TMPRSS2 protease activity. Here, we searched the genomes of metazoans from different classes using an extensive BLASTP survey and found ACE2 and TMPRSS2 occur in vertebrates, but some vertebrates lack Tmprss2. We identified 6 amino acids among 25 known human ACE2 residues are highly associated with the binding of ACE2 to SARS‐CoV‐2 ( p value < .01) by Fisher exact test, and following this, calculated the probability of viral attachment within each species by the randomForest function from R randomForest library. Furthermore, we observed that Ace2 selected from seven animals based on the above analysis lack the hydrophobic contacts identified on human ACE2, indicating less affinity of SARS‐CoV‐2 to Ace2 in animals than humans. Finally, the alignment of 3D structure between human ACE2 and other animals by I‐TASSER and TM‐align displayed a reasonable structure for viral attachment within these species. Taken together, our data may shed light on the human‐to‐animal transmission of SARS‐CoV‐2.

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Section: Resultsmentioning

confidence: 94%

Section: Summary Of Animals and Cells Affected By Sars-cov-2mentioning

confidence: 99%

Comparative analyses of ACE2 and TMPRSS2 gene: Implications for the risk to which vertebrate animals are susceptible to SARS‐CoV‐2

Huang

Jiang

Yan

2021

Journal of Medical Virology

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“…ACE2 is the primary receptor for SARS-CoV-2 cellular entry. [34] This receptor is widely expressed across multiple organs, including on epithelial cells of the nasopharynx, heart, lungs, kidney, intestine, central nervous system, and blood vessels. [35] Recent single-cell RNA-sequence (scRNA-seq) analysis revealed ACE2 gene expression in multiple epithelial cell types across the respiratory tract, most notably in nasal epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Age-related differences in SARS-CoV-2 binding factors: An explanation for reduced susceptibility to severe COVID-19 among children?

Abrehart

Suryadinata

McCafferty

et al. 2022

Paediatric Respiratory Reviews

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Context In contrast with other respiratory viruses, children infected with SARS-CoV-2 are largely spared from severe COVID-19. Objectives To critically assess age-related differences in three host proteins involved in SARS-CoV-2 cellular entry: angiotensin-converting enzyme 2 (ACE2), transmembrane serine protease 2 (TMPRSS2) and furin. Methods We systematically searched Medline, Embase, and PubMed databases for relevant publications. Studies were eligible if they evaluated ACE2, TMPRSS2 or furin expression, methylation, or protein level in children. Results Sixteen papers were included. Age-dependent differences in membrane-bound and soluble ACE2 were shown in several studies, with ACE2 expression increasing with age. TMPRSS2 and furin are key proteases involved in SARS-CoV-2 spike protein cleavage. TMPRSS2 expression is increased by circulating androgens and is thus low in pre-pubertal children. Furin has not currently been well researched. Limitations High levels of study heterogeneity. Conclusions Low expression of key host proteins may partially explain the reduced incidence of severe COVID-19 among children, although further research is needed.

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“…Using pseudoviruses enables research of highly pathogenic viruses without the requirement for highly secure biosafety facilities, which has greatly bene ted research on COVID-19 [47][48][49][50]. However, the previously reported SARS-CoV-2-related recombinant virus either simulated the functional structure of S and N protein or incorporated fragments of its nucleic acids, and failed to simulate the functional structure of complete SARS-CoV-2 particles.…”

Section: Introductionmentioning

confidence: 99%

Immunomolecular Assay Based on Selective Virion Capture by Spike Antibody and Viral Nucleic Acid Amplification for Detecting Intact SARS-CoV-2 Particles

Liu

Zhang

et al. 2022

Preprint

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Background: Effective therapeutics and vaccines for coronavirus disease 2019 (COVID-19) are currently lacking because of the mutation and immune escape of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Based on the propagation characteristics of SARS-CoV-2, rapid and accurate detection of complete virions from clinical samples and the environment is critical for assessing infection risk and containing further COVID-19 outbreaks. However, currently applicable methods cannot achieve large-scale clinical application because of factors such as the high viral load, cumbersome virus isolation steps, demanding environmental conditions, and long experimental periods. In this study, we developed an immunomolecular detection method combining capture of the viral spike glycoprotein with monoclonal antibodies and nucleic acid amplification via quantitative reverse transcription PCR to rapidly and accurately detect complete virions.Results: After constructing a novel pseudovirus, screening for specific antibodies, and optimizing the detection parameters, the assay achieved a limit of detection of 9 × 102 transduction units/mL of viral titer with high confidence (~95%) and excellent stability against human serum and common virus/pseudovirus. The coefficients of variation were 1.0–2.0% for intra-assay and inter-assay analyses, respectively. Compared with reverse transcription-PCR, the immunomolecular method more accurately quantified complete virions. SARS-CoV-2/pseudovirus was more stable on plastic and paper than on aluminum and copper in the detection of SARS-CoV-2 pseudovirus under different conditions, and complete virions were detected up to 96 h after they were applied to these surfaces (except for copper), although the titer of the virions was greatly reduced. Conclusions: Convenient, inexpensive, and accurate complete virus detection can be applied in many fields, such as monitoring the infectivity of convalescent and post-discharge patients and assessing high-risk environments (isolation rooms, operating rooms, patient living environments, and cold chain logistics). This method can also be used to detect intact virions such as those of hepatitis B and C viruses, human immunodeficiency virus, influenza, and the partial pulmonary virus, which may further improve the accuracy of diagnoses and facilitate individualized and precise treatments.

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Author Correction: Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

Abstract: A Correction to this paper has been published: https://doi.org/10.1038/s41467-021-22614-1.

Cited by 31 publications

References 0 publications

Comparative analyses of ACE2 and TMPRSS2 gene: Implications for the risk to which vertebrate animals are susceptible to SARS‐CoV‐2

Comparative analyses of ACE2 and TMPRSS2 gene: Implications for the risk to which vertebrate animals are susceptible to SARS‐CoV‐2

Age-related differences in SARS-CoV-2 binding factors: An explanation for reduced susceptibility to severe COVID-19 among children?

Immunomolecular Assay Based on Selective Virion Capture by Spike Antibody and Viral Nucleic Acid Amplification for Detecting Intact SARS-CoV-2 Particles

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