Author Correction: Analysis of gene network bifurcation during optic cup morphogenesis in zebrafish

Buono, Lorena; Corbacho, Jorge; Naranjo, Silvia; Almuedo-Castillo, María; Moreno-Mármol, Tania; Cerda, Berta de la; Sanabria-Reinoso, Estefanía; Polvillo, Rocío; Díaz-Corrales, Francisco-Javier; Bogdanović, Ozren; Bovolenta, Paola; Martínez-Morales, Juan-Ramón

doi:10.1038/s41467-021-24834-x

Cited by 2 publications

(3 citation statements)

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“…In that study, we showed that the consensus motif 5’-TAATT-3’, which is central to the neural retina cis -regulatory logic, is shared by many homeodomain TFs co-expressed during retinal specification; including not only vsx1 and vsx2 , but also rx1 , rx2 , rx3 , lhx2b , lhx9 , hmx1 , and hmx4 . Moreover, we show evidence that these TFs may co-regulate the same genes and cooperate within the same cis -regulatory modules ( Buono et al, 2021 ). According to these observations, gene redundancy appears as a more parsimonious explanation for the absence of an early phenotype in vsx KO embryos.…”

Section: Discussionmentioning

confidence: 83%

“…To this end, 18hpf embryo heads were collected from vsx KO and their wild type siblings and the rest of the tissue was used for PCR genotyping. We focused in this particular stage as it corresponds to the early bifurcation of the neural retina and RPE GRNs in zebrafish ( Buono et al, 2021 ). To first identify changes in the cis -regulatory landscape associated to vsx loss of function, we examined wild type and mutant samples using ATAC-seq.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous analysis of transcriptome dynamics and chromatin accessibility in segregating NR/RPE populations indicated that the regulatory networks involved in the specification of the zebrafish eye are remarkably robust ( Buono et al, 2021 ). In that study, we showed that the consensus motif 5’-TAATT-3’, which is central to the neural retina cis -regulatory logic, is shared by many homeodomain TFs co-expressed during retinal specification; including not only vsx1 and vsx2 , but also rx1 , rx2 , rx3 , lhx2b , lhx9 , hmx1 , and hmx4 .…”

Section: Discussionmentioning

confidence: 99%

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Mutation of vsx genes in zebrafish highlights the robustness of the retinal specification network

Letelier

Buono

Almuedo-Castillo

et al. 2023

eLife

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Genetic studies in human and mice have established a dual role for Vsx genes in retina development: an early function in progenitors’ specification, and a later requirement for bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent Vsx functions are also conserved across vertebrates, as mutant models are available only in mammals. To gain insight into vsx function in teleosts, we have generated vsx1 and vsx2 CRISPR/Cas9 double knockouts (vsxKO) in zebrafish. Our electrophysiological and histological analyses indicate severe visual impairment and bipolar cells depletion in vsxKO larvae, with retinal precursors being rerouted toward photoreceptor or Müller glia fates. Surprisingly, neural retina is properly specified and maintained in mutant embryos, which do not display microphthalmia. We show that although important cis-regulatory remodelling occurs in vsxKO retinas during early specification, this has little impact at a transcriptomic level. Our observations point to genetic redundancy as an important mechanism sustaining the integrity of the retinal specification network, and to Vsx genes regulatory weight varying substantially among vertebrate species.

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Section: Discussionmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mutation of vsx genes in zebrafish highlights the robustness of the retinal specification network

Letelier

Buono

Almuedo-Castillo

et al. 2023

eLife

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Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis

Moreno-Mármol

Ledesma-Terrón

Tabanera

et al. 2021

eLife

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The vertebrate eye-primordium consists of a pseudostratified neuroepithelium, the optic vesicle (OV), in which cells acquire neural retina or retinal pigment epithelium (RPE) fates. As these fates arise, the OV assumes a cup-shape, influenced by mechanical forces generated within the neural retina. Whether the RPE passively adapts to retinal changes or actively contributes to OV morphogenesis remains unexplored. We generated a zebrafish Tg(E1-bhlhe40:GFP) line to track RPE morphogenesis and interrogate its participation in OV folding. We show that, in virtual absence of proliferation, RPE cells stretch and flatten, thereby matching the retinal curvature and promoting OV folding. Localized interference with the RPE cytoskeleton disrupts tissue stretching and OV folding. Thus, extreme RPE flattening and accelerated differentiation are efficient solutions adopted by fast-developing species to enable timely optic cup formation. This mechanism differs in amniotes, in which proliferation drives RPE expansion with a much-reduced need of cell flattening.

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Author Correction: Analysis of gene network bifurcation during optic cup morphogenesis in zebrafish

Cited by 2 publications

References 0 publications

Mutation of vsx genes in zebrafish highlights the robustness of the retinal specification network

Mutation of vsx genes in zebrafish highlights the robustness of the retinal specification network

Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis

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