Author Correction: An engineered prime editor with enhanced editing efficiency in plants

Zong, Ying; Liu, Yijing; Xue, Chenxiao; Li, Boshu; Li, Xiangyang; Wang, Yanpeng; Li, Ji; Liu, Guanwen; Huang, Xingxu; Cao, Xiaofeng; Gao, Caixia

doi:10.1038/s41587-022-01308-z

Cited by 3 publications

(1 citation statement)

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“… 3 , 16 , 21 , 22 Further improvement of the highly engineered M-MLV RT in PE2 has also proven difficult, as all reported variants of this RT have also yielded minimal improvements in mammalian cell PE. 16 , 22 , 23 Although we reported that Cas9 mutations known to improve nuclease performance can also increase PE efficiency, 2 mutants of Cas9 identified specifically to improve PE have not yet been reported.…”

Section: Introductionmentioning

confidence: 87%

Phage-assisted evolution and protein engineering yield compact, efficient prime editors

Doman,

Pandey,

Neugebauer

et al. 2023

Cell

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Section: Introductionmentioning

confidence: 87%

Phage-assisted evolution and protein engineering yield compact, efficient prime editors

Doman,

Pandey,

Neugebauer

et al. 2023

Cell

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Development of a highly efficient prime editor 2 system in plants

Chen

Liang

et al. 2022

Genome Biol

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Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.

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Recent advances in therapeutic CRISPR-Cas9 genome editing: mechanisms and applications

Zhou

Yao

2023

Mol Biomed

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Recently, clustered regularly interspaced palindromic repeats (CRISPR)-Cas9 derived editing tools had significantly improved our ability to make desired changes in the genome. Wild-type Cas9 protein recognizes the target genomic loci and induced local double strand breaks (DSBs) in the guidance of small RNA molecule. In mammalian cells, the DSBs are mainly repaired by endogenous non-homologous end joining (NHEJ) pathway, which is error prone and results in the formation of indels. The indels can be harnessed to interrupt gene coding sequences or regulation elements. The DSBs can also be fixed by homology directed repair (HDR) pathway to introduce desired changes, such as base substitution and fragment insertion, when proper donor templates are provided, albeit in a less efficient manner. Besides making DSBs, Cas9 protein can be mutated to serve as a DNA binding platform to recruit functional modulators to the target loci, performing local transcriptional regulation, epigenetic remolding, base editing or prime editing. These Cas9 derived editing tools, especially base editors and prime editors, can introduce precise changes into the target loci at a single-base resolution and in an efficient and irreversible manner. Such features make these editing tools very promising for therapeutic applications. This review focuses on the evolution and mechanisms of CRISPR-Cas9 derived editing tools and their applications in the field of gene therapy.

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Author Correction: An engineered prime editor with enhanced editing efficiency in plants

Cited by 3 publications

References 0 publications

Phage-assisted evolution and protein engineering yield compact, efficient prime editors

Phage-assisted evolution and protein engineering yield compact, efficient prime editors

Development of a highly efficient prime editor 2 system in plants

Recent advances in therapeutic CRISPR-Cas9 genome editing: mechanisms and applications

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