Authentication of forensic DNA samples

Frumkin, Dan; Wasserstrom, Adam; Davidson, Ariane; Grafit, Arnon

doi:10.1016/j.fsigen.2009.06.009

Cited by 59 publications

(33 citation statements)

References 33 publications

(10 reference statements)

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“…The artificial DNA can be planted in and around crime scenes or applied to nearby objects or enmeshed with genuine human tissues (Frumkin et al, 2010;Melchior et al, 2008). To resolve the issue and authenticate the forensic DNA samples, sodium bisulfite treatment of DNA, followed by MSP or cloning and Sanger sequencing can be carried The DNA is spotted as a dot in the nylon membrane and then primary antibody is blotted, which is followed by a secondary antibody conjugated with horse radish peroxidase enzyme.…”

Section: Forensic Dna Samples Authenticationmentioning

confidence: 99%

“…The addition of enzyme's substrate such as 3'-3' diamino benzidine (DAB) produces a pink precipitate. Alternatively, a fluorophore-tagged secondary antibody (Alexa Fluor Ab) can be used to visualize the presence of methyl group out, which distinguishes the in vitro generated DNA versus genuine DNA (Frumkin et al, 2010).…”

Section: Forensic Dna Samples Authenticationmentioning

confidence: 99%

See 1 more Smart Citation

Crime investigation through DNA methylation analysis: methods and applications in forensics

Rana¹

2018

Egypt J Forensic Sci

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Discerning monozygotic twins, precisely predicting the age of human beings and most importantly interpreting human behavior remains a big challenge in forensic science. Modern advances in the field of epigenetics especially analysis of DNA methylation pattern can certainly help to identify and discriminate various single-source DNA samples such as bodily fluids collected from the crime scene. In this review, cytosine methylation analysis of DNA has been methodologically discussed with a broad range of applications in forensic science such as identifying single or mixed body fluid, discriminating monozygotic twins, age prediction, addiction/behavioral prediction as well as race/ethnicity identification. With the advances of next-generation sequencing techniques, blooming of DNA methylation datasets and together with standard epigenetic protocols, the prospect of investigating and solving these critical issues in forensic science is highly promising in the court of law.

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Section: Forensic Dna Samples Authenticationmentioning

confidence: 99%

Section: Forensic Dna Samples Authenticationmentioning

confidence: 99%

Crime investigation through DNA methylation analysis: methods and applications in forensics

Rana¹

2018

Egypt J Forensic Sci

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“…Niedawne badania epigenetyczne całego genomu pokazały skuteczność tej metody przy identyfikacji zmian o złożonych cechach u bliźniąt monozygotycznych [3]. Markery epigenetyczne pozwalają także na identyfikację płynów i tkanek ustrojowych, dokładne określanie wieku oraz wykrywanie sfabrykowanych dowodów DNA [12,[15][16][17].…”

Section: Markery Epigenetyczneunclassified

“…Recent genome-wide epigenetic studies displayed the power of this design to identify epigenetic changes with complex traits in monozygotic twins [3]. The epigenetic markers also enable to identify body fluid and tissue, identify the age accurately and detect fabricated DNA evidence [12,[15][16][17].…”

Section: Mikrorna I Niekodujące Małe Rnamentioning

confidence: 99%

Next generation sequencing (NGS): a golden tool in forensic toolkit

Aly¹,

Sabri²

2015

amsik

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StreszczenieAnaliza DNA stanowi fundament współczesnych nauk kryminalistycznych. Technologie sekwencjonowania DNA są skutecznymi narzędziami, które przyczyniają się do rozwoju nauk molekularnych. Dotychczas główną techniką sekwencjonowania była metoda Sangera. Dziś coraz większego znaczenia w naukach kryminalistycznych nabiera tzw. sekwencjonowanie nowej generacji (NGS). Metoda ta umożliwia rozwój i rozszerzanie zastosowań metod molekularnych w naukach kryminalistycznych dzięki eliminacji pułapek, które wiążą się z konwencjonalnymi metodami sekwencjonowania. Głównymi zaletami NGS w porównaniu z metodą konwencjonalną jest jednoczesne wykorzystywanie dużej liczby markerów genetycznych o wysokiej rozdzielczości danych genetycznych. Przyczyni się to niewątpliwie do rozwiązania kilku istotnych problemów, takich jak analiza mieszanin i badanie znikomych ilości materiału dla zdegradowanych próbek. Na bazie nowych technologii możliwa jest analiza wielu markerów w celu określenia istotnych cech biologicznych, np. wieku, pochodzenia geograficznego, typu tkanek i zewnętrznych cech fizycznych oraz identyfikacji bliźniąt monozygotycznych. Możliwe jest również uzyskanie danych dotyczących mikroorganizmów, owadów, roślin i gleby, które mają bardzo istotne znaczenie dla celów medyczno-sądowych. Mimo wielu badań z wykorzystaniem NGS dla celów sądowych rutynowe stosowanie tej technologii w sprawach kryminalistycznych wymaga jeszcze spełnienia określonych wymogów. Z tego względu niezbędne są dalsze badania analizujące efektywność zastosowania tych technik. W tym kontekście w niniejszej pracy omówiono najważniejsze zastosowania NGS w naukach kryminalistycznych w erze masowego sekwencjonowania równoległego.Słowa kluczowe: sekwencjonowanie nowej generacji, nauki kryminalistyczne, sekwencjonowanie metodą Sangera. AbstractThe DNA analysis is a cornerstone in contemporary forensic sciences. DNA sequencing technologies are powerful tools that enrich molecular sciences in the past based on Sanger sequencing and continue to glowing these sciences based on Next generation sequencing (NGS). Next generation sequencing has excellent potential to flourish and increase the molecular applications in forensic sciences by jumping over the pitfalls of the conventional method of sequencing. The main advantages of NGS compared to conventional method that it utilizes simultaneously a large number of genetic markers with high-resolution of genetic data. These advantages will help in solving several challenges such as mixture analysis and dealing with minute degraded samples. Based on these new technologies, many markers could be examined to get important biological data such as age, geographical origins, tissue type determination, external visible traits and monozygotic twins identification. It also could get data related to microbes, insects, plants and soil which are of great medico-legal importance. Despite the dozens of forensic research involving NGS, there are requirements before using this technology routinely in forensic cases. Thus, there is a ...

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“…Fingerprints are the most widely used biometric feature for identification person and verification in the field of biometrics 5 and used in forensic divisions worldwide for criminal investigations 3 , as well as DNA has become the gold standard of forensic testing and is an invaluable tool for the criminal 6 . Both of them used as the most accurate in forensic identification, what is more that the DNA can also as a gold standard for paternity testing 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Malang ve Madura, Dogu Java Endonezyasındaki Javalı ve Madurese"li Ailelerde Adli Profilleme

Iza¹,

Prawestiningtyas²,

Fatchiyah³

2014

Cukurova Medical Journal

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Purpose: The aims of this study are to identify the heritability of fingerprint patterns among three generations of Javanese and Madurese families and to determine the similarities, genetic variability and allele patterns for paternity testing. Material and Methods:The methods used in this study were the identification of the fingerprint patterns, DNA extraction from blood samples by salting out, PCR amplification use 13 CODIS which consists of TPOX, D3S1358, FGA, D5S818, CSF1PO, D7S820, D8S1179, TH01, VWA, D13S317, D16S539, D18S51, D21S11, and visualized by 8% polyacrylamide gel. The allele of individual band profile was analyzed by using QuantityOne software. Results:The results of fingerprint patterns showed that the families of Javanese ethnic has specific in ulnar patterns on both the middle and little fingers, meanwhile the families of Madurese ethnic has a plain whorl patterns on the right thumb and left index fingers which were inherited from the first generation to the next generation. The similarities of profile DNA forensic in Javanese ethnic generally have the same band patterns were produced by using D7S820 and CSF1PO markers, whereas in Madurese ethnic by using VWA and D18S51 markers. The genetic variability in Javanese ethnic by using D3S1358 and D21S11 markers, meanwhile the Madurese ethnic by using TH01 and D21S11 markers. Conclusion: We can conclude that there are the different characteristic of the fingerprint pattern of Javanese and Madurese families and the similarities and genetic variability in families of Javanese were different with the families of Madurese on the some markers.

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Authentication of forensic DNA samples

Cited by 59 publications

References 33 publications

Crime investigation through DNA methylation analysis: methods and applications in forensics

Crime investigation through DNA methylation analysis: methods and applications in forensics

Next generation sequencing (NGS): a golden tool in forensic toolkit

Malang ve Madura, Dogu Java Endonezyasındaki Javalı ve Madurese"li Ailelerde Adli Profilleme

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