Authentication of a selected medicinal plants using DNA barcoding technique

Abstract

Abstract. Plants are valuable source of a medicine and have long being used to cure various ailments. However, the efficacy of drugs derived from plant depends on the reliable identification of correct plants.To avoids the usage of incorrect plant that can cause poisoning, a reliable method than morphological characteristic is required. DNA barcoding technique have shown to be an efficient tool for species identification by using a short fragment of the genomic DNA and has been used widely in molecular plant taxonomy for authentication of medicinal plants species. Thus, the goal of this study was to use DNA barcoding technique to discriminate medicinal plants. DNA samples were extracted from twenty medicinal plants, chosen based on their therapeutic efficacy and were used as templates. Internal transcribed spacer (ITS2) gene was selected to be the best molecular marker for identification purposes. The efficiency of the amplification by polymerase chain reaction was sending for sequencing and species identification was performed using MEGA6. Our findings show that DNA barcoding is an efficient tool for plants identification. This study revealed that medicinal plant and their closely related species can be distinguished by using DNA barcoding technique with ITS2 region as it is an efficient marker and potential DNA marker for authentication of selected plants.