Autentificación de lapas del género Fissurella (Mollusca: Vetigastropoda) en la costa chilena, mediante PCR-RFLP

Olivares-Paz, Alberto; Quinteiro, Javier; Rey-Méndez, Manuel

doi:10.4067/s0717-71782006000100011

Cited by 7 publications

(7 citation statements)

References 6 publications

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“…Despite their importance as marine resources, there are few molecular genetic studies establishing phylogenetic relations between taxa or connectivity interaction between natural populations for the Chilean coasts. Existing studies have focused on analyzing allozymes (Guiñez et al 1992, Gallardo & Carrasco 1996, Véliz et al 2001, Gajardo et al 2002, PCR-RAPD (Marín et al 2007); PCR-RFLP (Olivares-Paz et al 2006) and isolation of microsatellite loci (Cárdenas et al 2007. To date, there are no specific sequence analyses of the distinct taxa.…”

Section: Introductionmentioning

confidence: 99%

MOLECULAR ANALYSIS IN CHILEAN COMMERCIAL GASTROPODS BASED ON 16S rRNA, COI AND ITS1-5.8S rDNA-ITS2 SEQUENCES

2009

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Gastropod mollusks are part of the principal marine resources cultivated and commercialized in Chile. There are native Chilean species such as loco (Concholepas concholepas), locate (Thais chocolata), trumulco snail (Chorus giganteus), keyhole limpets (Fissurella spp.), tegula snail (Tegula atra) as well as exotic species such as red abalone (Haliotis rufescens) and Japanese abalone (Haliotis discus hannai). Despite their importance as marine resources, molecular genetic studies establishing phylogenetic relationships and estimating population genetic parameters are scarce. The aim of this study is to establish a molecular approach among the main commercial gastropod species in Chile. The mitochondrial genes 16S rRNA and COI, and the nuclear ribosomal region ITS1-5.8SrDNA-ITS2 were amplified by PCR and sequencing. Alignment analysis was used to determine systematic relationships at the specific level for the species studied. The results revealed that 7 species are grouped in 4 genetically distinct families (Haliotidae, Trochidae, Muricidae and Fissurellidae). In comparison with COI sequencing, 16S rRNA and ITS1-5.8SrDNA-ITS2 sequencing were relatively more conserved with a divergence percentage for 16S rDNA and ITS1-5.8SrDNA-ITS2 of 1.2% and 1.8%, respectively, contrasting with the value of 10% obtained for COI in abalone.

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Section: Introductionmentioning

confidence: 99%

MOLECULAR ANALYSIS IN CHILEAN COMMERCIAL GASTROPODS BASED ON 16S rRNA, COI AND ITS1-5.8S rDNA-ITS2 SEQUENCES

2009

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“…Estas diferencias, tienen su origen en las variaciones morfológicas entre los individuos con la generación histórica de más de un nombre para la misma especie (Pérez-Farfante 1952, Marincovich 1973, Ramírez-Boehme 1974, McLean 1984. Aunque, McLean (1984), definió una serie de caracteres de valor taxonómico para las especies, con frecuencia ejemplares divergentes del holotipo descrito, de especies diferentes, comparten atributos morfológicos siendo difícil asignarlos a una determinada especie (Vegas-Velez 1968, Guzmán et al 1998, Olivares-Paz et al 2006. Previamente F. cumingi fue considerada una variedad de color de F. latimarginata y ambas especies comparten aspectos morfológicos de la concha y, en algunas variantes individuales, se encuentra algún patrón de la concha muy similar.…”

Section: Filogenéticaunclassified

“…Con el objetivo de llevar a cabo una autentificación genética de las diversas especies de lapas, destinadas a uso alimentario, el análisis de PCR-RFLP de secuencias del gen citocromo b mitocondrial mostró una incongruencia entre la taxonomía establecida y los datos patrones de RFLP observados. Así, especies taxonómicamente diferenciadas presentaron patrones de RFLP idénticos (Olivares-Paz et al 2006) La descripción formal de muchas especies no ha contado con el aporte de datos genético-moleculares debido a que han sido aplicados en las últimas dos décadas. Los estudios genéticos moleculares proveen mayor precisión en la determinación taxonómica y en la asignación de individuos a taxones concretos (Hillis 1987), siendo ambas técnicas complementarias y necesarias para la descripción de especies.…”

Section: Introductionunclassified

Filogenia molecular de las especies del subgénero Fissurella (Mollusca: Vetigastropoda) de la costa chilena

Olivares-Paz

Quinteiro

Rey-Méndez

2011

Rev. biol. mar. oceanogr.

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Abstract.-Several species of the genus Fissurella coexist on the rocky intertidal substrate in the southeastern coast of the Pacific Ocean. Thirteen species are considered as valid, considering some variable and morphologically unstable species, which have historically allowed registering 58 specific names. This report describes the congruence degree existing between the current typological classification and molecular data obtained from nuclear and mitochondrial genes. Data includes sequences of the spacers ITS1 and ITS2 in the nuclear ribosomal operon and partial sequences of the mitochondrial gene ND1. Reconstruction of phylogenetic relations shows congruence between mitochondrial and nuclear data and allows us to clearly establish two groups of species, including polyphyletic species. The first group contains the 'latimarginata complex', which includes morphotypes of F. latimarginata, F. cumingi, F. bridgesii and F. oriens. In addition, the monophyletic species F. radiosa, F. pulchra and F. peruviana are included in this group. The second group corresponds to the complex 'costata-picta' that includes F. costata and F. picta. Other monophyletic species in this group were F. maxima in an intermediate position, F. limbata and F. nigra in a basal position. According to our results, F. crassa was situated in a basal position, i.e., close to root, within the phylogenetic tree. This data indicates the necessity of a taxonomic revision of this genus.Key words: Taxonomy, Fissurellidae, sympatric species, genesResumen.-Varias especies del género Fissurella coexisten en simpatría en el sustrato rocoso de la región suroriental del Océano Pacífico. La taxonomía actual basada en caracteres morfológicos variables e inestables, considera 13 especies válidas para este género, los cuales históricamente han permitido registrar 58 nombres específicos. Este trabajo evalúa el grado de congruencia existente entre la actual clasificación tipológica y los datos moleculares obtenidos a partir de genes mitocondriales y nucleares. Estos datos incluyen la secuencia parcial del gen mitocondrial ND1 y la de los espaciadores ITS1 e ITS2 del operón ribosomal nuclear. La reconstrucción de las relaciones filogenéticas muestra una congruencia entre datos mitocondriales y nucleares y permite establecer claramente dos grupos de especies, que incluyen especies polifiléticas. En el primero se localiza el 'complejo latimarginata', que incluye indistintamente morfotipos de F. latimarginata, F. cumingi, F. bridgesii y F. oriens. Junto a esas especies polifiléticas se sitúan las monofiléticas F. radiosa, F. pulchra y F. peruviana. En el segundo grupo se sitúa el complejo 'costata-picta' que incluye a F. costata y F. picta. Las otras especies monofiléticas de este grupo son F. maxima, en posición intermedia F. limbata y F. nigra en posición basal. La especie F. crassa se sitúa en posición basal, i.e., próximo a la raíz del árbol filogenético. Estos datos indican la necesidad de una revisión taxonómica de este género.

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“…are very common species in rocky intertidal shores of Northwest Europe and the Mediterranean, occurring in a wide gradient of wave exposure. As many other intertidal invertebrates, limpets have been traditionally exploited for consumption in many places around the world such as Mexico and South of the United States (Pombo and Escofet, 1996), Hawaii (McCoy, 2008), Canary Islands (Moro and Herrera, 2000), Azores (Martins et al, 1987) and Chile (Olivares-Paz et al, 2006). In some areas, the high demand and the lack of an adequate planning for a sustainable exploitation, such in Canary Island, or the deficient enforcement of the conservation measures, such in Azores, has originated important overexploitation issues, which have led to the commercial extinction of some species (Official Bulletin of the the reproductive cycle and population dynamics in the Portuguese and British coasts indicate a high intra-and inter-specific variability regarding parameters such as density, growth rate, reproductive cycle and sex ratio (Baxter, 1983;Morais et al, 2003;McCarthy et al, 2008;Ribeiro et al, 2009), correlating with abiotic parameters, such as latitude, wave exposure or substrate height and slope, as well as on biotic parameters, due to competence with other species.…”

Section: Introductionmentioning

confidence: 98%

Characterization of the reproductive cycle and physiological condition of Patella vulgata in the NW of the Iberian Peninsula: Relevant information for a sustainable exploitation

2015

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Autentificación de lapas del género Fissurella (Mollusca: Vetigastropoda) en la costa chilena, mediante PCR-RFLP

Cited by 7 publications

References 6 publications

MOLECULAR ANALYSIS IN CHILEAN COMMERCIAL GASTROPODS BASED ON 16S rRNA, COI AND ITS1-5.8S rDNA-ITS2 SEQUENCES

MOLECULAR ANALYSIS IN CHILEAN COMMERCIAL GASTROPODS BASED ON 16S rRNA, COI AND ITS1-5.8S rDNA-ITS2 SEQUENCES

Filogenia molecular de las especies del subgénero Fissurella (Mollusca: Vetigastropoda) de la costa chilena

Characterization of the reproductive cycle and physiological condition of Patella vulgata in the NW of the Iberian Peninsula: Relevant information for a sustainable exploitation

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