Aurora kinase-C-T191D is constitutively active mutant

Abstract

BackgroundAurora kinases (Aurora-A, B and C) belong to a family of conserved serine/threonine kinases which are key regulators of cell cycle progression. Aurora-A and Aurora-B are expressed in somatic cells and involved in cell cycle regulation while aurora-C is meiotic chromosome passenger protein. As Aurora kinase C is rarely expressed in normal somatic cells and has been found over expressed in many cancer lines. It is suggested that Aurora-C-T191D is not hyperactive mutant.ResultAurora-C-T191D variant form was investigated and compared with wild type. The overexpression of Aurora-C-T191D was observed that it behaves like Aurora-C wild type (aurC-WT). Both Aurora-C-T191D and aurC-WT induce abnormal cell division resulting in centrosome amplification and multinucleation in transiently transfected cells as well as in stable cell lines. Similarly, Aurora-C-T191D and aurC-WT formed foci of colonies when grown on soft agar, indicating that a gain of Aurora-C activity is sufficient to transform cells. Furthermore, we reported that NIH-3 T3 stable cell lines overexpressing Aurora-C-T191D and its wild type partner induced tumour formation when injected into nude mice, demonstrating the oncogenic activity of enzymatically active Aurora kinase C. Interestingly enough tumour aggressiveness was positively correlated with the rate of kinase activity, making Aurora-C a potential anti-cancer therapeutic target.ConclusionThese findings proved that Aurora C-T191D is not hyperactive but is constitutively active mutant.

Functions of Aurora kinase C in meiosis and cancer

“…Overexpression increases cellular proliferation and migration and enhances xenograft tumor growth (Tsou et al, 2011 ). Kinase-dead AURKC decreases proliferation of HeLa cells while expression of the constitutively active AURKC (Spengler, 2007a ; Khan et al, 2012 ) leads to more aggressive tumors (Khan et al, 2011 ; Tsou et al, 2011 ). Other carcinogenic genes are also located in the telomeric region of human Chromosome 19 (Bernard et al, 1998 ), a genomic region susceptible to translocations and deletions (Bernard et al, 1998 ; Kimura et al, 1999 ).…”

Erratum to: Aurora kinase-C-T191D is constitutively active mutant

“…The editors regretfully retract the article [ 1 ] by Jabbar Khan, Sanaullah Khan, Sobia Attaullah, Ijaz Ali, and Shahid Khan (BMC Cell Biology 2012, 13:8) due to significant overlap with previously published article “Overexpression of Active Aurora-C Kinase Results in Cell Transformation and Tumour Formation” by Jabbar Khan, Frédéric Ezan, Jean-Yves Crémet, Alain Fautre, David Gilot, Marine Lambert, Christelle Benaud, Marie-Bérengère Troadec, and Claude Prigent (PLoS ONE 6(10): e26512. doi:10.1371/journal.pone.0026512).…”

Investigation of waste banana peels and radish leaves for their biofuels potentialInvestigation of waste banana peels and radish leaves for their biofuels potential

“…Previously, the biodiesel and bioethanol are reported from different bioresources including wheat, maize, sugar beet, potatoes, palm tree, sun flower, fruits and vegetable wastes, and algae [1][2][3][4]. The biodiesel is obtained from bioresources that are rich in oily content.…”