Aurora kinase B modulates chromosome alignment in mouse oocytes

Shuda, Kristy; Schindler, Karen; Ma, Jun; Schultz, Richard M.; Donovan, Peter

doi:10.1002/mrd.21075

Cited by 85 publications

(114 citation statements)

References 58 publications

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“…It is intriguing that the passenger proteins are not detected in the centromere regions as they are in mitotic and centrosomal meiotic cells. Our results are consistent with data from C. elegans oocytes (Kaitna et al 2002;Rogers et al 2002;Monen et al 2005) and mouse oocytes (Shuda et al 2009;Sharif et al 2010), showing that the CPC interacts with noncentromeric chromatin at metaphase of meiosis I. In C. elegans, the CPC forms a ring at the center of each bivalent that colocalizes with cohesion proteins distal to chiasmata Figure 6 Model for the relationship between the central spindle, spindle bipolarity, and centromere orientation during acentrosomal spindle assembly.…”

Section: Cpc Promotes Spindle Assembly and Establishes The Spindle Axissupporting

confidence: 88%

“…Furthermore, in mouse (Dumont et al 2007) and Drosophila melanogaster (Cesario and Mckim 2011) oocytes, RanGTP may be dispensable for meiosis I spindle assembly. On the other hand, chromosome alignment and segregation are defective after knockdown of the CPC in both mouse and Caenorhabditis elegans oocytes, but spindle assembly has not been closely examined (Schumacher et al 1998;Speliotes et al 2000;Kaitna et al 2002;Rogers et al 2002;Shuda et al 2009;Sharif et al 2010).…”

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confidence: 99%

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The Chromosomal Passenger Complex Is Required for Meiotic Acentrosomal Spindle Assembly and Chromosome Biorientation

2012

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During meiosis in the females of many species, spindle assembly occurs in the absence of the microtubule-organizing centers called centrosomes. In the absence of centrosomes, the nature of the chromosome-based signal that recruits microtubules to promote spindle assembly as well as how spindle bipolarity is established and the chromosomes orient correctly toward the poles is not known. To address these questions, we focused on the chromosomal passenger complex (CPC). We have found that the CPC localizes in a ring around the meiotic chromosomes that is aligned with the axis of the spindle at all stages. Using new methods that dramatically increase the effectiveness of RNA interference in the germline, we show that the CPC interacts with Drosophila oocyte chromosomes and is required for the assembly of spindle microtubules. Furthermore, chromosome biorientation and the localization of the central spindle kinesin-6 protein Subito, which is required for spindle bipolarity, depend on the CPC components Aurora B and Incenp. Based on these data we propose that the ring of CPC around the chromosomes regulates multiple aspects of meiotic cell division including spindle assembly, the establishment of bipolarity, the recruitment of important spindle organization factors, and the biorientation of homologous chromosomes.

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Section: Cpc Promotes Spindle Assembly and Establishes The Spindle Axissupporting

confidence: 88%

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confidence: 99%

The Chromosomal Passenger Complex Is Required for Meiotic Acentrosomal Spindle Assembly and Chromosome Biorientation

2012

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“…AURKB is also expressed in mammalian oocytes (Jelinkova & Kubelka 2006, Swain et al 2008, Uzbekova et al 2008, Shuda et al 2009). It gradually becomes phosphorylated on threonine 232 (Thr232) during oocyte maturation.…”

Section: Introductionmentioning

confidence: 99%

Progesterone receptor membrane component 1 expression and putative function in bovine oocyte maturation, fertilization, and early embryonic development

Luciano¹,

Lodde²,

Franciosi³

et al. 2010

Reproduction

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Although the mRNA that encodes progesterone receptor membrane component 1 (PGRMC1) is present in mammalian oocytes, nothing is known about either PGRMC1's expression pattern or function in oocytes during maturation, fertilization, and subsequent embryonic development. As PGRMC1 associates with the mitotic spindle in somatic cells, we hypothesized that PGRMC1 is involved in oocyte maturation (meiosis). Western blot analysis confirmed the presence of PGRMC1 in bovine oocytes. This study also shows that PGRMC1 is present at the germinal vesicle (GV)-and MII-stage oocytes and is associated with male and female pronucleus formation of the zygote and is highly expressed in blastocysts. A more detailed examination of PGRMC1 localization using confocal imaging demonstrated that in GV-stage oocytes, PGRMC1 was concentrated throughout the GV but did not localize to the chromatin. With the resumption of meiosis in vitro, PGRMC1 concentrated in the centromeric region of metaphase I chromosomes, while in the anaphase I/telophase I stages the majority of PGRMC1 concentrated between the separating chromosomes. At the metaphase II stage, PGRMC1 re-associated with the centromeric region of the chromosomes. A colocalization study demonstrated that PGRMC1 associated with the phosphorylated form of aurora kinase B, which localizes to the centromeres at metaphase. Finally, PGRMC1 antibody injection significantly lowered the percentage of oocytes that matured and reached the metaphase II stage after 24 h of culture. The majority of the PGRMC1 antibodyinjected oocytes arrested in the prometaphase I stage of meiosis. Furthermore, in most of the PGRMC1 antibody-injected oocytes, the chromosomes were disorganized and scattered. Taken together, these data demonstrate that PGRMC1 is expressed in bovine oocytes and its localization changes at specific stages of oocyte maturation. These observations suggest an important role for PGRMC1 in oocyte maturation, which may be specifically related to the mechanism by which chromosomes segregate.

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“…According to a previous report, AURKB was responsible for regulating chromosome dynamics during meiosis in mouse oocytes. AURKB inhibition resulted in defects in meiotic progression and chromosome alignment at both metaphase of meiosis I (Met I) and metaphase of meiosis II (Met II), while over-expression of AURKB reversed the chromosome alignment defect, suggesting that AURKB is the primary Aurora kinase responsible for regulating chromosome dynamics during meiosis in mouse oocytes [3]. Our results indicate that AURKB was required for the development of mouse zygotes.…”

Section: Discussionmentioning

confidence: 69%

“…Inhibition of Aurora kinases resulted in aberrant chromatin remodeling and significantly fewer cells progressing to meiosis II (MII). AURKA over-expression triggered abnormal amplification of centrosomes, while AURKB-over-expressed oocytes failed in proper chromosomal arrangement [2,3]. These studies prompted us to question how Aurora kinases would function during development after fertilization.…”

mentioning

confidence: 99%

AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development

Lin

Tong

Feng³

et al. 2012

Sci. China Life Sci.

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Aurora kinases have become a hot topic for research as they have been found to play an important role in various stages of mitotic cell division and to participate in malignant conversions of tumors. The participation of Aurora kinases in the regulation of oocyte meiosis has been recently reported, but their participation in mammalian early embryonic development remained unclear. The object of our study was to establish the spatio-temporal expression pattern of Aurora kinase B (AURKB) in mouse zygotes during the first cleavage, to reveal its functions in the early development of mouse zygotes, and to define the involvement of AURKB in mitogen-activated protein kinase (MAPK) signaling. Our results showed that in mouse zygotes AURKB expression increased in G1 phase and peaked in M phase. AURKB protein distribution was found to be in association with nuclei and distributed throughout the cytoplasm in a cell cycle-dependent manner. Functional disruption of AURKB resulted in abnormal division phenotypes or mitotic impairments. U0126, a specific mitogen-activated protein kinase kinase (MEK) inhibitor, caused significantly altered morphologies of early embryos together with a decrease in protein expression and kinase activity of AURKB. Our results indicated that the activity of AURKB was required for regulating multiple stages of mitotic progression in the early development of mouse zygotes and was correlated with the activation of the MAPK pathway. AURKB, MAPK, mouse zygote, mitosis, cell cycle regulation Citation:Xu L, Liu T, Han F, et al. AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development. Sci China Life Sci, 2012, 55: 47 -56,

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Aurora kinase B modulates chromosome alignment in mouse oocytes

Cited by 85 publications

References 58 publications

The Chromosomal Passenger Complex Is Required for Meiotic Acentrosomal Spindle Assembly and Chromosome Biorientation

The Chromosomal Passenger Complex Is Required for Meiotic Acentrosomal Spindle Assembly and Chromosome Biorientation

Progesterone receptor membrane component 1 expression and putative function in bovine oocyte maturation, fertilization, and early embryonic development

AURKB and MAPK involvement in the regulation of the early stages of mouse zygote development

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