Aurora B kinase is required for cell cycle progression in silkworm

Gang, Xiaoxu; Qian, Wenliang; Zhang, Tianlei; Yang, Xinxin; Xia, Qingyou; Cheng, Daojun

doi:10.1016/j.gene.2016.11.015

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…Cell cycle and apoptosis analysis was also conducted, as cell cycle arrest and cell apoptosis were considered as two major events of cell growth inhibition. 21,38 The cell cycle distribution of HepG2 cells was analyzed by flow cytometer after incubation with CMSN at a dose of 0-100 μg/mL. As revealed in Figure 8C, the cell cycle distribution of the treated groups was similar to the untreated samples.…”

Section: In Vitro Toxicitymentioning

confidence: 78%

<p>Superiority of L-tartaric Acid Modified Chiral Mesoporous Silica Nanoparticle as a Drug Carrier: Structure, Wettability, Degradation, Bio-Adhesion and Biocompatibility</p>

Hu¹,

Wang²,

Li³

et al. 2020

IJN

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Purpose: The purpose of this research was to study the basic physicochemical and biological properties regarding the application of L-tartaric acid modified chiral mesoporous silica nanoparticle (CMSN) as a drug carrier, and to explore the structure-property relationship of silica-based materials. Methods: CMSN with functions of carboxyl modification and chirality was successfully synthesized through co-condensation method, and the basic characteristics of CMSN, including morphology, structure, wettability, degradation, bio-adhesion and retention ability in gastrointestinal tract (GI tract) were estimated by comparing with non-functionalized mesoporous silica nanoparticles (MSN). Meanwhile, the biocompatibility and toxicity of L-tartaric modification were systematically evaluated both in vitro and in vivo through MTT cell viability assay, cell cycle and apoptosis assay, hemolysis assay, histopathology examination, hematology analysis, and clinical chemistry examination. Results: CMSN and MSN were spherical nanoparticles with uniform mesoporous structure. CMSN with smaller pore size and carboxyl functional groups exhibited better wettability. Besides, CMSN and MSN could dissolve thoroughly in simulated physiological fluids during a degradation period of 1-12 weeks. Interestingly, the in vitro and in vivo behaviors of carriers, including degradation, bio-adhesion and retention ability in the GI tract were closely related to wettability. As expected, CMSN had faster degradation rate, higher mucosa-adhesion ability, and longer retention time. Particularly, CMSN improved the bio-adhesion property in both gastric mucosa and small intestinal mucosa, and prolonged the GI tract retention time to at least 12 h, which meant higher probability for absorption. The biocompatibility and toxicity examination indicated that CMSN was a kind of biocompatible bio-material with good blood compatibility and negligible toxicity, which is required for further applications in biological fields. Conclusion: CMSN with functions of carboxyl modification and chirality had superiority in terms of both physicochemical and biological properties. The in vitro and in vivo behaviors of carriers, including degradation, bio-adhesion, and retention were closely related to wettability.

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Section: In Vitro Toxicitymentioning

confidence: 78%

<p>Superiority of L-tartaric Acid Modified Chiral Mesoporous Silica Nanoparticle as a Drug Carrier: Structure, Wettability, Degradation, Bio-Adhesion and Biocompatibility</p>

Hu¹,

Wang²,

Li³

et al. 2020

IJN

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“…Aurora B is constitutively expressed in mitotically active cells and up-regulated in highly proliferative tissues. A previous study [34] revealed high expression of the BmAurora B gene in the testis and ovary of silkworms during the final larval instar. This coincided with the cell proliferation phase of both female and male gametogenesis in silkworms.…”

Section: Discussionmentioning

confidence: 89%

siRNA-Mediated BmAurora B Depletion Impedes the Formation of Holocentric Square Spindles in Silkworm Metaphase BmN4 Cells

Zhang,

Ayra-Pardo,

Liu

et al. 2024

Insects

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Silkworm ovary-derived BmN4 cells rely on chromatin-induced spindle assembly to form microtubule-based square mitotic spindles that ensure accurate segregation of holocentric chromosomes during cell division. The chromosome passenger protein Aurora B regulates chromosomal condensation and segregation, spindle assembly checkpoint activation, and cytokinesis; however, its role in holocentric organisms needs further clarification. This study examined the architecture and dynamics of spindle microtubules during prophase and metaphase in BmN4 cells and those with siRNA-mediated BmAurora B knockdown using immunofluorescence labeling. Anti-α-tubulin and anti-γ-tubulin antibodies revealed faint γ-tubulin signals colocalized with α-tubulin in early prophase during nuclear membrane rupture, which intensified as prophase progressed. At this stage, bright regions of α-tubulin around and on the nuclear membrane surrounding the chromatin suggested the start of microtubules assembling in the microtubule-organizing centers (MTOCs). In metaphase, fewer but larger γ-tubulin foci were detected on both sides of the chromosomes. This resulted in a distinctive multipolar square spindle with holocentric chromosomes aligned at the metaphase plate. siRNA-mediated BmAurora B knockdown significantly reduced the γ-tubulin foci during prophase, impacting microtubule nucleation and spindle structure in metaphase. Spatiotemporal BmAurora B expression analysis provided new insights into the regulation of this mitotic kinase in silkworm larval gonads during gametogenesis. Our results suggest that BmAurora B is crucial for the formation of multipolar square spindles in holocentric insects, possibly through the activation of γ-tubulin ring complexes in multiple centrosome-like MTOCs.

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“…The 10 and 100 μM morroniside-treated groups exhibited a pronounced increase in the ratio of BrdU + /cTnT + cells relative to the OGD group ( Figures 1B, D ). To determine whether increased DNA replication in cardiomyocytes ultimately leads to increased karyokinesis and cytokinesis, we stained the cells with phospho-histone H3 (pH3), a marker of late G2-phase/mitosis ( Bhaskar et al, 2018 ), and Aurora B, a kinase localized in the midbody during cytokinesis ( Gang et al, 2017 ). As indicated in Figures 1E–H , the percentages of pH3 + /cTnT + and Aurora B + /cTnT + cells in the 10 μM and 100 μM morroniside-treated groups were higher than those in the OGD group.…”

Section: Resultsmentioning

confidence: 99%

Morroniside induces cardiomyocyte cell cycle activity and promotes cardiac repair after myocardial infarction in adult rats

Zheng,

Liu,

Chen

et al. 2024

Front. Pharmacol.

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Introduction: Acute myocardial infarction (AMI) is characterized by the loss of cardiomyocytes, which impairs cardiac function and eventually leads to heart failure. The induction of cardiomyocyte cell cycle activity provides a new treatment strategy for the repair of heart damage. Our previous study demonstrated that morroniside exerts cardioprotective effects. This study investigated the effects and underlying mechanisms of action of morroniside on cardiomyocyte cell cycle activity and cardiac repair following AMI.Methods: Neonatal rat cardiomyocytes (NRCMs) were isolated and exposed to oxygen-glucose deprivation (OGD) in vitro. A rat model of AMI was established by ligation of the left anterior descending coronary artery (LAD) in vivo. Immunofluorescence staining was performed to detect newly generated cardiomyocytes. Western blotting was performed to assess the expression of cell cycle-related proteins. Electrocardiography (ECG) was used to examine pathological Q waves. Masson’s trichrome and wheat germ agglutinin (WGA) staining assessed myocardial fibrosis and hypertrophy.Results: The results showed that morroniside induced cardiomyocyte cell cycle activity and increased the levels of cell cycle proteins, including cyclin D1, CDK4, cyclin A2, and cyclin B1, both in vitro and in vivo. Moreover, morroniside reduced myocardial fibrosis and remodeling.Discussion: In conclusion, our study demonstrated that morroniside stimulates cardiomyocyte cell cycle activity and cardiac repair in adult rats, and that these effects may be related to the upregulation of cell cycle proteins.

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Aurora B kinase is required for cell cycle progression in silkworm

Cited by 9 publications

References 38 publications

<p>Superiority of L-tartaric Acid Modified Chiral Mesoporous Silica Nanoparticle as a Drug Carrier: Structure, Wettability, Degradation, Bio-Adhesion and Biocompatibility</p>

<p>Superiority of L-tartaric Acid Modified Chiral Mesoporous Silica Nanoparticle as a Drug Carrier: Structure, Wettability, Degradation, Bio-Adhesion and Biocompatibility</p>

siRNA-Mediated BmAurora B Depletion Impedes the Formation of Holocentric Square Spindles in Silkworm Metaphase BmN4 Cells

Morroniside induces cardiomyocyte cell cycle activity and promotes cardiac repair after myocardial infarction in adult rats

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