Aurantiamide acetate from baphicacanthus cusia root exhibits anti-inflammatory and anti-viral effects via inhibition of the NF-κB signaling pathway in Influenza A virus-infected cells

Zhou, Beixian; Yang, Zifeng; Feng, Qitong; Liang, Xiaoli; Li, Jing; Zanin, Mark; Jiang, Zhi‐Hong; Zhong, Nanshan

doi:10.1016/j.jep.2017.01.038

Cited by 64 publications

(48 citation statements)

References 38 publications

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“…In addition, the biological activities and medical applications of these five known compounds ( 2 – 6 ) had been reported by the following literatures. Compound 2 could suppressed hind paw swelling in the adjuvant‐arthritic rat model, while also having antioxidant, anti‐neuroinflammatory, anti‐inflammatory, anti‐inflammatory, anti‐inflammatory and antiviral effects . Antimycobacterial potency against the Mycobacterium tuberculosis strain H37Rv and impressive anti‐inflammatory activity were reported for compounds 3 .…”

Section: Resultsmentioning

confidence: 99%

A New Alkaloid from the Aerial Parts of Bupleurum chinense DC.

Xiao

Liu

Sun

et al. 2020

Chemistry & Biodiversity

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A new isoalloxazine alkaloid, named bupleurine A (1), along with five known compounds (2–6), were isolated from the aerial parts of Bupleurum chinense DC. The structure elucidation of the new alkaloid (1) was employed by combining NMR and HR‐MS data with comparison of reference in the literature. Five known compounds (2–6) were isolated from Bupleurum genus for the first time. Additionally, their antiproliferative activities on HeLa cells were evaluated by MTT assay and IC50 of compounds 1 and 4–6 were below 10 μm after treatment for 24 h.

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Section: Resultsmentioning

confidence: 99%

A New Alkaloid from the Aerial Parts of Bupleurum chinense DC.

Xiao

Liu

Sun

et al. 2020

Chemistry & Biodiversity

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“…Tryptanthrin was first reported as the distinct antiviral agent to reduce HCoV-NL63-induced CPE and to drastically inhibit the progeny virus production in a concentration-dependent manner (IC50 = 1.52 μM) ( Figure 4). Previous studies revealed several active antiviral compounds in the S. cusia extract against Herpes simplex virus-1 (IC50 = 49.3 μM by lupeol) [4], and influenza A virus (IC50 = 29.2 μM by strobilanthes A, IC50 = 46.0 μM by 2(3H)-benzoxazolinone, and IC50 = 31.48-71.57 μg/mL by aurantiamide acetate) [5,6]. Previous studies revealed several active antiviral compounds in the S. cusia extract against Herpes simplex virus-1 (IC50 = 49.3 μM by lupeol) [4], and influenza A virus (IC50 = 29.2 μM by strobilanthes A, IC50 = 46.0 μM by 2(3H)-benzoxazolinone, and IC50 = 31.48-71.57 μg/mL by aurantiamide acetate) [5,6].…”

Section: Discussionmentioning

confidence: 99%

“…S. cusia root and leaf extracts have been used extensively as a traditional herbal medicine with antiinflammatory, antipyretic, antimicrobial, and antiviral activities [2,3]. The S. cusia root (named "Nan-Ban-Lan-Gen" in Chinese) has been commonly used to treat infections by respiratory virus, such as influenza viruses, mumps virus, and severe acute respiratory syndrome (SARS) coronavirus [4,5]. Several bioactive components from the S. cusia root, including strobilanthes A, 3H-benzoxazolinone, and aurantiamide acetate, have exhibited antiviral activity against influenza A and hepatitis B virus infections [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…The S. cusia root (named "Nan-Ban-Lan-Gen" in Chinese) has been commonly used to treat infections by respiratory virus, such as influenza viruses, mumps virus, and severe acute respiratory syndrome (SARS) coronavirus [4,5]. Several bioactive components from the S. cusia root, including strobilanthes A, 3H-benzoxazolinone, and aurantiamide acetate, have exhibited antiviral activity against influenza A and hepatitis B virus infections [5,6]. The S. cusia leaf (called "Da-Ching-Yeh" in Chinese) is generally used for the production of indigo dyes (Indigo Natruralis, named "Qing Dai" in Chinese), displaying antibacterial, anti-inflammatory, and antipyretic properties [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Antiviral Action of Tryptanthrin Isolated from Strobilanthes cusia Leaf against Human Coronavirus NL63

et al. 2020

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Strobilanthes cusia (Nees) Kuntze is a Chinese herbal medicine used in the treatment of respiratory virus infections. The methanol extract of S. cusia leaf contains chemical components such as β-sitosterol, indirubin, tryptanthrin, betulin, indigodole A, and indigodole B that have diverse biological activities. However, the antiviral action of S. cusia leaf and its components against human coronavirus remains to be elucidated. Human coronavirus NL63 infection is frequent among immunocompromised individuals, young children, and in the elderly. This study investigated the anti-Human coronavirus NL63 (HCoV-NL63) activity of the methanol extract of S. cusia leaf and its major components. The methanol extract of S. cusia leaf effectively inhibited the cytopathic effect (CPE) and virus yield (IC50 = 0.64 μg/mL) in HCoV-NL63-infected cells. Moreover, this extract potently inhibited the HCoV-NL63 infection in a concentration-dependent manner. Among the six components identified in the methanol extract of S. cusia leaf, tryptanthrin and indigodole B (5aRethyltryptanthrin) exhibited potent antiviral activity in reducing the CPE and progeny virus production. The IC50 values against virus yield were 1.52 μM and 2.60 μM for tryptanthrin and indigodole B, respectively. Different modes of time-of-addition/removal assay indicated that tryptanthrin prevented the early and late stages of HCoV-NL63 replication, particularly by blocking viral RNA genome synthesis and papain-like protease 2 activity. Notably, tryptanthrin (IC50 = 0.06 μM) and indigodole B (IC50 = 2.09 μM) exhibited strong virucidal activity as well. This study identified tryptanthrin as the key active component of S. cusia leaf methanol extract that acted against HCoV-NL63 in a cell-type independent manner. The results specify that tryptanthrin possesses antiviral potential against HCoV-NL63 infection.

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“…The crude extracts and pure phytochemicals of S. cusia leave (Da-Ching-Yeh or Nan-Ban-Lan-Yeh) and root (Na-Ban-Lan-Gen) showed many pharmacological properties, including antiviral (anti-herpes simplex virus type-1, anti-influenza A virus), antisevere acute respiratory syndrome (SARS), antifungal, anti-inflammatory, antinociceptive, and antipyretic effects (Feng et al, 2016; https://doi.org/10. 1016/j.jep.2020.112772 Received 3 September 2019; Received in revised form 5 March 2020; Accepted 15 March 2020 Gu et al, 2015;Ho et al, 2003;Honda and Tabata, 1979;Tanaka et al, 2004;Zhou et al, 2017). To date many secondary metabolites, such as alkaloids, terpenoids, flavonoids, sterols, lignans, phenylethanoids, and isocoumarin have been isolated from this species (Feng et al, 2016;Gu et al, 2015;Honda and Tabata, 1979;Tanaka et al, 2004;Zhou et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

IL-17A inhibitions of indole alkaloids from traditional Chinese medicine Qing Dai

Lee

Wang

Kuo

et al. 2020

Journal of Ethnopharmacology

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A B S T R A C TEthnopharmacological relevance: Qing Dai, a famous traditional Chinese medicine (TCM), is prepared by a traditional fermentation process with the aerial part of Strobilanthes cusia. Currently, this TCM could treat various clinical inflammatory diseases, such as ulcerative colitis and psoriasis, however, the bioactive components of Qing Dai are unknown clearly. Aim of the study: To isolate and identify the anti-IL-17A components of Qing Dai. Materials and methods: Silica, RP-18 gels, and size exclusion resin were used for column chromatography to isolate the pure compounds. The structures of isolates were elucidated by NMR, MS, UV, IR spectra, and optical rotation. IL-17A protein and gene expressions were also evaluated in the Th17 cell model and luciferase reporter assay, respectively. Results: Two indole alkaloids, including one new indigodole D and cephalandole B, were isolated from Qing Dai. Indigodole D could inhibit IL-17A protein production during the Th17 polarization (EC 50 : 2.16 μg/mL) or after the polarization (EC 50 : 5.99 μg/mL) without cytotoxicity toward Th17 cells. Cephalandole B did not inhibit the IL-17A protein secretion. Nevertheless, both isolates notably inhibited IL-17A gene expression, especially cephalandole B, in a dose-dependent manner in Jukat cells with IL-17A luciferase reporter. Conclusions: Indole alkaloids, indigodoles A, C, D, tryptanthrin, and indirubin could contribute to anti-IL 17A properties of Qing Dai. The possible biogenetic mechanisms of above-mentioned indoles were also speculated in this investigation for further promising anti-IL-17 lead drugs development.

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Aurantiamide acetate from baphicacanthus cusia root exhibits anti-inflammatory and anti-viral effects via inhibition of the NF-κB signaling pathway in Influenza A virus-infected cells

Cited by 64 publications

References 38 publications

A New Alkaloid from the Aerial Parts of Bupleurum chinense DC.

A New Alkaloid from the Aerial Parts of Bupleurum chinense DC.

Antiviral Action of Tryptanthrin Isolated from Strobilanthes cusia Leaf against Human Coronavirus NL63

IL-17A inhibitions of indole alkaloids from traditional Chinese medicine Qing Dai

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