Abstract-The purpose of this study was to identify changes in venomotor tone in the chronic low-dose angiotensin II (Ang II) model of hypertension and to establish the contribution of sympathetic nerve activation to these venomotor tone changes. Male Sprague-Dawley rats were acclimatized to a 0.4% or 2.0% NaCl diet for 7 days and then catheterized to allow chronic and repeated measures of arterial pressure, central venous pressure, and mean circulatory filling pressure (MCFP), an index of venous smooth muscle tone, in conscious undisturbed rats. After 4 days of recovery and a 3-day control period, an Ang II or physiological saline-filled osmotic minipump was implanted subcutaneously to deliver Ang II (150 ng/kg per minute) or vehicle control for 14 days. MCFP was measured in duplicate before and after acute ganglionic blockade with hexamethonium (30 mg/kg IV) on control day 2 and Ang II infusion on days 1, 3, 7, and 14. Blood volume was also measured on these days and was unchanged for the duration of the study in all of the groups. Arterial pressure was increased for the duration of Ang II infusion in rats on both 0.4% and 2% NaCl diets, but the increase was significantly greater in the 2% NaCl group and completely abolished by hexamethonium. MCFP was significantly increased for the entire Ang II infusion period only in rats fed 2% NaCl, and this increase was completely abolished by hexamethonium. We conclude that the combination of chronic low-dose Ang II infusion and high dietary salt intake engages the sympathetic nervous system to increase venomotor tone. Key Words: angiotensin II Ⅲ venomotor tone Ⅲ mean circulatory filling pressure Ⅲ sympathetic nervous system Ⅲ salt E merging evidence suggests that sympathetic nervous system activation may represent a common neurogenic mechanism of hypertension in both human essential hypertension 1-6 and many experimental animal models. 7-10 Angiotensin II (Ang II) has been identified as a humoral factor implicated in activating the sympathetic nervous system in human hypertension 11,12 ; and the pressor response to infusion of chronic low-dose Ang II in animals has been shown, at least in part, to be sympathetically driven. [13][14][15] Advances have been made in the elucidation of the central pathways involved in mediating this sympathoexcitatory effect, suggesting that systemically delivered Ang II likely activates critical circumventricular organs 16 -19 with efferent projections to brain centers known to influence sympathetic nervous system activity. 18 Oxidative stress may mediate this central sympathoexcitatory effect. 20 However, there is still substantial uncertainty as to the critical peripheral target and the hemodynamic response to this increased sympathetic activity.In this study, we investigated the possibility that the venous circulation may be an important target for Ang II-induced sympathetic nervous system activation. The venous system contains Ϸ70% of the blood volume, 21 mostly in the small veins and venules, and has been shown to be more sensitive t...