Augmented inhibitory effect of superoxide dismutase on superoxide anion release from macrophages by direct cationization

Takagi, Toshihide; Kitano, Makiko; Masuda, Sada; Tokuda, Hideaki; Takakura, Yoshinobu; Hashida, Mitsuru

doi:10.1016/s0304-4165(96)00125-0

Cited by 9 publications

(2 citation statements)

References 25 publications

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“…Since Felgner (8) reported efficient gene expression using cationic lipids for the transfection of cells with plasmid DNA, cationic molecule-based delivery systems for plasmid DNA have been extensively investigated in an attempt to achieve nonviral gene transfer to target cells (33). In addition, enzymes such as SOD (27,28,40,48), glucose oxidase (21), and catalase (21,43), as well as serum albumins (32), immunoglobulins (52), and ferritin (7), all of which are negatively charged at physiological pH, have been directly modified with diamines to obtain cationized derivatives. Cationized proteins exhibit increased cellular uptake by brain microvascular endothelial cells, hepatocytes, kidney epithelial cells, and enterocytes.…”

Section: Discussionmentioning

confidence: 99%

Electrical charge on protein regulates its absorption from the rat small intestine

Nishikawa¹,

Hasegawa²,

Yamashita³

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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The effect of the electrical charge on the intestinal absorption of a protein was studied in normal adult rats. Chicken egg lysozyme (Lyz), a basic protein with a molecular weight of 14,300, was selected and several techniques for chemical modification were applied. Then the intestinal absorption of Lyz derivatives was evaluated by measuring the radioactivity in plasma and tissues, after the administration of an (111)In-labeled derivative to an in situ closed loop of the jejunum. After the administration of (111)In-Lyz, the level of radioactivity in plasma was comparable with the lytic activity of Lyz, supporting the fact that the radioactivity represents intact Lyz. (111)In-cationized Lyz showed a 2-3 times higher level of radioactivity in plasma, whereas the radioactivity of (111)In-anionized Lyz was much lower. The absorption rate of (111)In-Lyz derivatives calculated by a deconvolution method was correlated for the strength of their positive net charge. A similar relationship was observed using superoxide dismutase. These findings indicate that the intestinal absorption of a protein is, at least partially, determined by its electrical charge.

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Section: Discussionmentioning

confidence: 99%

Electrical charge on protein regulates its absorption from the rat small intestine

Nishikawa¹,

Hasegawa²,

Yamashita³

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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show abstract

“…Recently, the potential utility of Ad35 vectors as vaccine vectors has been proposed, 29,30 and DC and macrophages are considered to be ideal targets for immunotherapy using Ad35 vector-based vaccines. Bone marrow-derived dendritic cells 31 and peritoneal macrophages 32 were prepared as Figure 1 Human CD46 expression in organs harvested from CD46TG mice. The protein samples were prepared from wild-type mice (0n), hemizygous CD46TG mice (1n) and homozygous CD46TG mice (2n).…”

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confidence: 99%

Adenovirus serotype 35 vector-mediated transduction into human CD46-transgenic mice

et al. 2006

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We previously demonstrated that systemic administration of adenovirus serotype 35 (Ad35) vectors to mice does not mediate efficient transduction in organs, probably because expression of the mouse analog of the subgroup B Ad receptor, human CD46 (membrane cofactor protein), is limited to the testis. Here, we describe the in vitro and in vivo transduction characteristics of Ad35 vectors by using homozygous and hemizygous human CD46-transgenic (CD46TG) mice, which ubiquitously express human CD46. An Ad35 vector more efficiently transduced the primary dendritic cells and macrophages prepared from CD46TG mice than those from wild-type mice. In vivo transduction experiments demonstrated that CD46TG mice are more susceptible to Ad35 vector-mediated in vivo transduction than are wild-type mice. In particular, homozygous CD46TG mice, which express higher levels of CD46 in the organs than hemizygous CD46TG mice, tend to exhibit higher transduction efficiencies after intraperitoneal administration than hemizygous CD46TG mice. Intraperitoneal administration of Ad35 vectors resulted in efficient transduction into the mesothelial cells of the peritoneal organs in homozygous CD46TG mice. These results indicate that an Ad35 vector recognizes human CD46 as a cellular receptor in CD46TG mice. However, the in vivo transduction efficiencies of Ad35 vectors in CD46TG mice are much lower than those of conventional Ad5 vectors in wild-type mice.

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Targeted Delivery of Superoxide Dismutase by Chemical Modification with Quaternary Ammonium Chitosan and Pharmacokinetic Analysis

Liu

Wang²,

et al. 2015

J Pharm Innov

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Augmented inhibitory effect of superoxide dismutase on superoxide anion release from macrophages by direct cationization

Cited by 9 publications

References 25 publications

Electrical charge on protein regulates its absorption from the rat small intestine

Electrical charge on protein regulates its absorption from the rat small intestine

Adenovirus serotype 35 vector-mediated transduction into human CD46-transgenic mice

Targeted Delivery of Superoxide Dismutase by Chemical Modification with Quaternary Ammonium Chitosan and Pharmacokinetic Analysis

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