Augmentation of Atrazine biodegradation by two Bacilli immobilized on α-Fe2O3 magnetic nanoparticles

Abstract

In this study, a novel immobilizing carrier with α-Fe2O3 magnetic nanoparticles was developed and used for immobilization of atrazine-degrading bacterial isolates of Bacillus spp. Since the free cells of microorganisms generally not succeed to degrade pollutants; thus, extra treatments are alluring to make strides biodegradation. Scanning electron microscope (SEM) images appeared that after immobilization the bacterial cells were totally retained and entirely distributed on the surface of α-Fe2O3 magnetic nanoparticles. The performance of α-Fe2O3 immobilized cells in atrazine (ATZ) degradation was compared with the free cells, which was about 90.56% in 20 days. Experimental results exhibited that ATZ could be degraded at a broad range of physicochemical parameters viz. pH (4.0 to 9.0), temperature (20 to 45 °C), ATZ concentration (50 to 300 mg L−1) and agitation speed (50 to 300 rpm), which underlines that α-Fe2O3 immobilized cells could tolerate a higher range of ATZ concentration as compared to free cells. This research demonstrated that α-Fe2O3 could be applied as a potential carrier in cell immobilization and biodegradation of ATZ herbicide with greater efficiency.

An automated real-time microfluidic platform to probe single NK cell heterogeneity and cytotoxicity on-chip

“…Recently, several other alternative approaches have been developed, e.g. fluorescent probes to detect cell viability 6 , metabolic MTT assay 7 , enzyme-based LDH assays 8 , and luciferase transduced cell-based bioluminescence assays 9 . Even though these assays have provided insight into fundamental cellular behaviour, these studies are performed in bulk and generate averaged responses 10 .…”

The Bioremediation Capacity of Sphingomonas melonis for Methomyl‐Contaminated Soil Media: RSM Optimization and Biochemical Assessment by Dreissena polymorpha

“…They determined that this bacterial isolate could biodegrade the imidacloprid insecticide with an effective efficiency of over 90 %. Similarly, Khatoon et al [27] . used Bacillus bacteria species for the biodegradation of atrazine and optimization of the parameters used during these processes by RSM.…”

Biocatalytic System Made of 3D Chitin, Silica Nanopowder and Horseradish Peroxidase for the Removal of 17α-Ethinylestradiol: Determination of Process Efficiency and Degradation Mechanism

“…Among various enzymes' supports, inorganic particles are widely used due to their advantageous properties [13][14][15][16]. One of the most interesting supports for enzyme immobilization is SiO 2 .…”