Augmentation and dispersion of thein vivo mitotic apparatus of living marine eggs

Rebhun, Lionel I.; Sawada, Nobuaki

doi:10.1007/bf01247894

Cited by 37 publications

(13 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enlarged monopolar aster and centrosphere were also observed during fertilization by means of taxol injection (our unpublished data) and incubation in a taxolcontaining medium (21). The effects of taxol on the mitotic structures quite resemble those of hexyleneglycol on the mitotic apparatus with respect to changes in distribution of microtubule-organizing granules and in morphology of microtubular cytoskeletons for asters and spindle (5,17).…”

Section: Discussionmentioning

confidence: 62%

Redistribution of fluorescently labeled tubulin in the mitotic apparatus of sand dollar eggs and the effects of taxol.

Hamaguchi

Toriyama

Sakai

et al. 1987

Cell Struct. Funct.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 62%

Redistribution of fluorescently labeled tubulin in the mitotic apparatus of sand dollar eggs and the effects of taxol.

Hamaguchi

Toriyama

Sakai

et al. 1987

Cell Struct. Funct.

View full text Add to dashboard Cite

“…Instead, taxol seemed to promote the nucleation of microtubules that remained partially dynamic since at the onset of mitosis the bundles were disrupted and replaced by small asters. Hexyleneglycol, another compound known to reduce the critical tubulin concentration for microtubule assembly (Rebhun and Sawada, 1969) also induced the formation of microtubule asters specifically in the mitotic cytoplasm of sea urchin eggs and disrupted the spindle (Endo et al, 1990). Therefore, lowering the critical tubulin concentration for microtubule assembly has a differential effect in interphase and mitosis.…”

mentioning

confidence: 99%

Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein.

Verde

Berrez

Antony

et al. 1991

The Journal of Cell Biology

290

200

View full text Add to dashboard Cite

Abstract. Taxol, a microtubule stabilizing drug, induces the formation of numerous microtubule asters in the cytoplasm of mitotic cells (De Brabander, M., G. Geuens, R. Nuydens, R. Willebrords, J. DeMey. 1981. Proc. Natl. Acad. Sci. USA. 78:5608-5612). The center of these asters share with spindle poles some characteristics such as the presence of centrosomal material and calmodulin. We have recently reproduced the assembly of taxol asters in a cell-free system (Buendia, B., C. Antony, F. Verde, M. Bornens, and E. . J. Cell Sci. 97:259-271) using extracts of Xenopus eggs. In this paper, we show that taxol aster assembly requires phosphorylation, and that they do not grow from preformed centers, but rather by a reorganization of microtubules first crosslinked into bundles. This process seems to involve sliding of microtubules along each other and we show that cytoplasmic dynein is required for taxol aster assembly. This result provides a possible functional basis to the recent findings, that dynein is present in the spindle and enriched near spindle poles (Pfarr, C. M., M. Cove, P. M. Grissom, T. S. Hays, M. E. Porter, and

show abstract

“…Quantification of spindle birefringence under various physical and pharmacological conditions provided key insights into the reversible assembly of the mitotic spindle (reviewed in [135,136,137]). A sharp dependency of spindle birefringence on temperature suggested a low energy bonding between aligned subunits of the birefringent spindle fibers and provided compelling evidence for the self-assembly of fibers at physiological temperatures [135].…”

Section: Insights Into Mitotic Mechanism From Studies Of Mitotic Pmentioning

confidence: 99%

A Brief History of Research on Mitotic Mechanisms

McIntosh

Hays

2016

Biology

View full text Add to dashboard Cite

This chapter describes in summary form some of the most important research on chromosome segregation, from the discovery and naming of mitosis in the nineteenth century until around 1990. It gives both historical and scientific background for the nine chapters that follow, each of which provides an up-to-date review of a specific aspect of mitotic mechanism. Here, we trace the fruits of each new technology that allowed a deeper understanding of mitosis and its underlying mechanisms. We describe how light microscopy, including phase, polarization, and fluorescence optics, provided descriptive information about mitotic events and also enabled important experimentation on mitotic functions, such as the dynamics of spindle fibers and the forces generated for chromosome movement. We describe studies by electron microscopy, including quantitative work with serial section reconstructions. We review early results from spindle biochemistry and genetics, coupled to molecular biology, as these methods allowed scholars to identify key molecular components of mitotic mechanisms. We also review hypotheses about mitotic mechanisms whose testing led to a deeper understanding of this fundamental biological event. Our goal is to provide modern scientists with an appreciation of the work that has laid the foundations for their current work and interests.

show abstract

Augmentation and dispersion of thein vivo mitotic apparatus of living marine eggs

Cited by 37 publications

References 31 publications

Redistribution of fluorescently labeled tubulin in the mitotic apparatus of sand dollar eggs and the effects of taxol.

Redistribution of fluorescently labeled tubulin in the mitotic apparatus of sand dollar eggs and the effects of taxol.

Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein.

A Brief History of Research on Mitotic Mechanisms

Contact Info

Product

Resources

About