“…The tissue and cell protein were extracted by the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime Institute of Biotechnology) according to the manufacturer's instructions. Western blots were performed according to our previous work (Wu Q. Q. et al, 2018). Primary antibodies, including NLRP3 (ab214185, Abcam), HMGB1 (ab79823, Abcam), glyceraldehyde 3-phosphate dehydrogenase (2118, CST, MA, United States), Lamin B (ab16048, Abcam), β-actin (ab8227, Abcam), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 2 (ab129068, Abcam), NOX4 (ab154244, Abcam), and SOD2 (ab68155, Abcam) were used in Western blot.…”