Au/ZnO Nanocomposites Prepared by Laser Ablation for Enhancement of Antibacterial Activity and Cytotoxic Properties against Cancer Cells

Abstract

This study presents a comprehensive look into the potential therapeutic, antibacterial, and anticancer properties of a nanocomposite (NC) of gold (Au) and zinc oxide (ZnO). In this study, we analyzed the adherence between Au nanoparticles (NPs) and ZnO NPs. X-ray diffraction analysis showed high crystallinity and small crystallite sizes of Au NPs and ZnO NPs, while transmission electron microscopy showed spherical NPs. Furthermore, histogram analysis showed that the average particle size of Au NPs is 27 nm, while that of ZnO NPs is 35 nm. The adherence of ZnO NPs on the surface of Au NPs increased their combined particle size to 51 nm and revealed a high-population core-shell structure. The activity of Au/ZnO NCs against Escherichia coli was more potent when compared to that of elemental Au and ZnO NPs alone. The cytotoxic effects of Au NPs, ZnO NPs, and Au/ZnO NCs against human breast cancer cells (AMG13) and human esophageal adenocarcinoma cancer cells (SK-GT4) were investigated in this study. We found that AMG13 is more sensitive than SK-GT4 to the activity of Au/ZnO NCs. The cytotoxicity of Au/ZnO NCs against AMG13 was 89%, while that against SK-GT4 was 85%. Less cytotoxic effects were recorded against normal cells (MCF7) when compared with those of cancer cells. Based on these findings, the synthesized Au/ZnO NCs could be used as a promising strategy for biomedical applications.

Antibacterial and hemocompatibility potentials of nano-gold-cored alginate preparation against anaerobic bacteria from acne vulgaris

“…04-0784 and 01-074-0895. These peaks confirmed the crystalline nature of the GANPs and provided confirmatory proof that the material can be indexed as a face-centered-cubic structure 42 , 43 . Out of the related plane, the (111) plane showed a higher intensity than other planes, demonstrating that the (111) plane is the typical orientation.…”

Synthesis and characterization of mesoporous zinc oxide nanoparticles and evaluation of their biocompatibility in L929 fibroblasts

“…Recently, ZnO NPs have gained significant attention in different medical and dental fields due to their exceptional antibacterial properties (Alhujaily et al, 2022 ; Moradpoor et al, 2021 ). In a previous investigation, the therapeutic potential of a nanocomposite (NC) comprising ZnO NPs and gold (Au) nanoparticles (NPs) was examined (Alhujaily et al, 2023 ). The NC was extensively characterized, revealing a core‐shell structure formed by the adherence of ZnO NPs onto the surface of Au NPs (Alhujaily et al, 2023 ).…”

“…In a previous investigation, the therapeutic potential of a nanocomposite (NC) comprising ZnO NPs and gold (Au) nanoparticles (NPs) was examined (Alhujaily et al, 2023 ). The NC was extensively characterized, revealing a core‐shell structure formed by the adherence of ZnO NPs onto the surface of Au NPs (Alhujaily et al, 2023 ). Notably, the Au/ZnO NCs exhibited improved antibacterial activity against Escherichia coli compared to individual Au and ZnO NPs (Alhujaily et al, 2023 ).…”

“…The NC was extensively characterized, revealing a core‐shell structure formed by the adherence of ZnO NPs onto the surface of Au NPs (Alhujaily et al, 2023 ). Notably, the Au/ZnO NCs exhibited improved antibacterial activity against Escherichia coli compared to individual Au and ZnO NPs (Alhujaily et al, 2023 ). Moreover, the therapeutic efficacy of the synthesized Au/ZnO NCs was assessed on human breast cancer cells and human esophageal adenocarcinoma cancer cells (Alhujaily et al, 2023 ).…”

“…Notably, the Au/ZnO NCs exhibited improved antibacterial activity against Escherichia coli compared to individual Au and ZnO NPs (Alhujaily et al, 2023 ). Moreover, the therapeutic efficacy of the synthesized Au/ZnO NCs was assessed on human breast cancer cells and human esophageal adenocarcinoma cancer cells (Alhujaily et al, 2023 ). These findings indicate that the synthesized Au/ZnO NCs hold great potential for various biomedical applications (Alhujaily et al, 2023 ).…”