Au@SiO2 SERS nanotags based lateral flow immunoassay for simultaneous detection of aflatoxin B1 and ochratoxin A

Chen, Ruipeng; Wang, Hui; Sun, Chaoqun; Zhao, Yiguang; He, Yong; Nisar, Muhammad Shemyal; Wei, Wensong; Kang, Ho-Min; Xie, Xiulan; Du, Chunmei; Luo, Qingyao; Yang, Liang; Tang, Xiangfang; Xiong, Bin

doi:10.1016/j.talanta.2023.124401

Cited by 17 publications

(8 citation statements)

References 51 publications

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“…The LOD for AFB 1 and OTA was 0.24 and 0.37 ng kg −1 using the approach and recoveries from spiked agricultural samples were within the range of 87-112%. [104] Furthermore, the multiplexed, portable analysis of six mycotoxins: AFB 1 , DON, ZEA, FB 1 , OTA, and T-2 in maize samples was developed by Zhang et al using an SERS-based lateral flow immunosensor, which utilized dual Raman labels and triple test lines (Figure 11e). In this approach, two Raman reporter molecules, DTNB and 4-MBA, were used to label Au@Ag core-shell nanoparticles for the preparation of SERS nanoprobes.…”

Section: Antibodiesmentioning

confidence: 99%

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Advancing Mycotoxin Detection in Food and Feed: Novel Insights from Surface‐Enhanced Raman Spectroscopy (SERS)

Logan,

Cao,

Freitag

et al. 2024

Advanced Materials

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The implementation of low‐cost and rapid technologies for the on‐site detection of mycotoxin‐contaminated crops is a promising solution to address the growing concerns of the agri‐food industry. Recently, there have been significant developments in Surface‐enhanced Raman spectroscopy (SERS) for the direct detection of mycotoxins in food and feed. This review provides an overview of the most recent advancements in the utilization of SERS through the successful fabrication of novel nanostructured materials. Various bottom‐up and top‐down approaches have demonstrated their potential in improving sensitivity, while many applications exploit the immobilization of recognition elements and molecular imprinted polymers (MIPs) to enhance specificity and reproducibility in complex matrices. Therefore, the design and fabrication of nanomaterials is of utmost importance and are presented herein. We uncover that limited studies establish limits of detection (LODs) or conduct validation using naturally contaminated samples. One decade on, SERS is still lacking significant progress and there is a disconnect between the technology, the European regulatory limits, and the intended end‐user. Ongoing challenges and potential solutions are discussed including nanofabrication, molecular binders, and data analytics. Recommendations to assay design, portability, and substrate stability are made to help improve the potential and feasibility of SERS for future on‐site agri‐food applications.This article is protected by copyright. All rights reserved

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Section: Antibodiesmentioning

confidence: 99%

“…Reproduced with permission. [ 104 ] Copyright 2023, Elsevier. e) Illustration of multiplex SERS‐based lateral flow immunosensor for the detection of six mycotoxins (ZEA, AFB 1 , DON, FB 1 , T‐2, and OTA) in maize.…”

Section: Application Of Molecular Binders For the Detection Of Mycoto...mentioning

confidence: 99%

Advancing Mycotoxin Detection in Food and Feed: Novel Insights from Surface‐Enhanced Raman Spectroscopy (SERS)

Logan,

Cao,

Freitag

et al. 2024

Advanced Materials

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“…The design of SERS substrates should be tailored to the specific target substances. In food safety applications, the target substances can be categorized into two groups: small amount molecules [ 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 ] and tiny biomaterials, such as cells [ 89 , 90 , 91 , 92 ], cell walls [ 93 , 94 , 95 , 96 ], and spores [ 97 , 98 , 99 , 100 ]. The key distinction between these two categories lies in their size.…”

Section: Design Fabrication and Applications Of Sersmentioning

confidence: 99%

Design, Fabrication, and Applications of SERS Substrates for Food Safety Detection: Review

Lin

et al. 2023

Micromachines

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Sustainable and safe food is an important issue worldwide, and it depends on cost-effective analysis tools with good sensitivity and reality. However, traditional standard chemical methods of food safety detection, such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and tandem mass spectrometry (MS), have the disadvantages of high cost and long testing time. Those disadvantages have prevented people from obtaining sufficient risk information to confirm the safety of their products. In addition, food safety testing, such as the bioassay method, often results in false positives or false negatives due to little rigor preprocessing of samples. So far, food safety analysis currently relies on the enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), HPLC, GC, UV-visible spectrophotometry, and MS, all of which require significant time to train qualified food safety testing laboratory operators. These factors have hindered the development of rapid food safety monitoring systems, especially in remote areas or areas with a relative lack of testing resources. Surface-enhanced Raman spectroscopy (SERS) has emerged as one of the tools of choice for food safety testing that can overcome these dilemmas over the past decades. SERS offers advantages over chromatographic mass spectrometry analysis due to its portability, non-destructive nature, and lower cost implications. However, as it currently stands, Raman spectroscopy is a supplemental tool in chemical analysis, reinforcing and enhancing the completeness and coverage of the food safety analysis system. SERS combines portability with non-destructive and cheaper detection costs to gain an advantage over chromatographic mass spectrometry analysis. SERS has encountered many challenges in moving toward regulatory applications in food safety, such as quantitative accuracy, poor reproducibility, and instability of large molecule detection. As a result, the reality of SERS, as a screening tool for regulatory announcements worldwide, is still uncommon. In this review article, we have compiled the current designs and fabrications of SERS substrates for food safety detection to unify all the requirements and the opportunities to overcome these challenges. This review is expected to improve the interest in the sensing field of SERS and facilitate the SERS applications in food safety detection in the future.

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“…The integrated reaction column was prepared by 3D printing technology, and the number of channels could be designed according to experimental requirements, reducing time, and development costs. Chen, Wang, et al (2023) have recently developed a SERS-based LFA that allowed the simultaneous determination of AFB 1 and OTA in maize with detection limits of 0.24 and 0.37 pg/mL, respectively.…”

Section: Signal Analyzersmentioning

confidence: 99%

“…Chen, Wang, et al. (2023) have recently developed a SERS‐based LFA that allowed the simultaneous determination of AFB 1 and OTA in maize with detection limits of 0.24 and 0.37 pg/mL, respectively.…”

Section: Signal Analyzersmentioning

confidence: 99%

Improvement of the sensitivity of lateral flow systems for detecting mycotoxins: Up‐to‐date strategies and future perspectives

Li,

Jallow,

Nidiaye

et al. 2023

Comp Rev Food Sci Food Safe

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Mycotoxins are dangerous human and animal health‐threatening secondary fungal metabolites that can be found in various food and agricultural products. Several countries have established regulations to restrict their presence in food and agricultural products destined for human and animal consumption. Consequently, the need to develop highly sensitive and smart detection systems was recognized worldwide. Lateral flow assay possesses the advantages of easy operation, rapidity, stability, accuracy, and specificity, and it plays an important role in the detection of mycotoxins. Nevertheless, strategies to comprehensively improve the sensitivity of lateral flow assay to mycotoxins in food have rarely been highlighted and discussed. In this article, a comprehensive overview was presented on the application of lateral flow assay in mycotoxin detection in food samples by highlighting the principle of lateral flow assay, presenting a detailed discussion on various analytical performance‐improvement strategies, such as the development of high‐affinity recognition reagents, immunogen immobilization methods, and signal amplification. Additionally, a detailed discussion on the various signal analyzers and interpretation approaches was provided. Finally, current hurdles and future perspectives on the application of lateral flow assay in the detection of mycotoxins were discussed.

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Au@SiO2 SERS nanotags based lateral flow immunoassay for simultaneous detection of aflatoxin B1 and ochratoxin A

Cited by 17 publications

References 51 publications

Advancing Mycotoxin Detection in Food and Feed: Novel Insights from Surface‐Enhanced Raman Spectroscopy (SERS)

Advancing Mycotoxin Detection in Food and Feed: Novel Insights from Surface‐Enhanced Raman Spectroscopy (SERS)

Design, Fabrication, and Applications of SERS Substrates for Food Safety Detection: Review

Improvement of the sensitivity of lateral flow systems for detecting mycotoxins: Up‐to‐date strategies and future perspectives

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