Au@Pt nanostructures as oxidase and peroxidase mimetics for use in immunoassays

He, Weiwei; Liu, Ying; Jiang, Yuan; Yin, Jun‐Jie; Wu, Xiaochun; Hu, Xiaona; Zhang, Ke; Li, Jianbo; Chen, Chunying; Ji, Yinglu; Guo, Yuting

doi:10.1016/j.biomaterials.2010.09.040

Cited by 532 publications

(337 citation statements)

References 40 publications

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“…The results revealed that our proposed method was competitive with the well-established ELISA. It has been reported that a series of peroxidase-like nanoparticles have been used in colorimetric immunoassay, such as Fe 3 O 4 nanoparticles [30], Au@Pt nanostructures [31], Fe-aminoclay [32], Pt nanoparticles [33], and Pt@mSiO 2 nanoparticles [34]. By the comparison shown in ESM Table S2, our developed colorimetric immunoassay provides a potentially novel and sensitive strategy for H-IgG detection.…”

Section: Analytical Performances Of the Colorimetric Immunoassaymentioning

confidence: 93%

Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity

et al. 2015

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A naked-eye sensitive ELISA-like assay was developed based on gold-enhanced peroxidase-like activity of gold nanoparticles (AuNPs). Using human IgG (H-IgG) as an analytical model, goat anti-human IgG antibody (anti-IgG) adsorbed on microtiter plate and AuNPs-labeled anti-IgG acted as capture antibody and detection antibody, respectively. Because the surfaces of AuNPs were blocked by protein molecules, the peroxidase-like activity of AuNPs was almost inhibited, evaluated by the catalytic oxidation of peroxidase enzyme substrate 3,3′,5,5′-tetramethylbenzidine (TMB), which could produce a bright blue color in the presence of H 2 O 2 . Fortunately, the catalytic ability of AuNPs was dramatically increased by the deposition of gold due to the formation of a new gold shell on immunogold. Under optimal reaction conditions, the colorimetric immunoassay presented a good linear relationship in the range of 0.7-100 ng/mL and the limit of detection (LOD) of 0.3 ng/mL calculated by 3σ/S for UVvis detection, and obtained LOD of 5 ng/mL for naked-eye detection. The obtained results were competitive with conventional sandwich ELISA with the LOD of 1.6 ng/mL. Furthermore, this developed colorimetric immunoassay was successfully applied to diluted human serum and fetal bovine serum samples, and predicted a broad prospect for the use of peroxidase-like activity involving nanomaterials in bioassay and diagnostics.

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Section: Analytical Performances Of the Colorimetric Immunoassaymentioning

confidence: 93%

Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity

et al. 2015

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“…More in- teresting, we found that apart from catalase-like activity, Au NRs core/Pt NPs shell (Au@Pt NRs) have intrinsic oxidaseand peroxidase-like activities (Figures 9(c) and (d)). By replacing HRP in ELISA, they exhibited sensitive detection for mouse interleukin 2 with a LOD in several pg/mL (Figure 10) [23].…”

Section: Noble Metal Nanoparticles As Sod Catalase Peroxidase and Omentioning

confidence: 99%

“…With the rapid development in this field, more nanomaterials have been found to exhibit enzyme mimetics, such as bimetallic nanostructures [21][22][23][24], ferritin-platinum [25], positively charged gold NPs [26], graphene oxide and single wall carbon nanotube [27,28]. Herein, we present an overview of significant advances in this field and highlight the future perspectives.…”

mentioning

confidence: 97%

Research progress of nanoparticles as enzyme mimetics

Hou

Wen

et al. 2011

Sci. China Phys. Mech. Astron.

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“…Au@Pt nanomaterials with hydrogen peroxide (H 2 O 2 ) have been reported as peroxidase mimetic in immunoassays and glucose sensing. 19,20 Several studies have reported the intrinsic peroxidase mimicking activity of nanostructured materials that can be used in traditional enzyme-linked immunosorbent assay method after replacing horseradish peroxidase with CeO 2 , Pt, carbon, and magnetic nanoparticles. [21][22][23][24] Gold nanostructures have recently been useful in bio-applic ations because of their nontoxicity, ease of surface modification, polyvalent effects, and photothermal capacity.…”

Section: Ramasamy Et Almentioning

confidence: 99%

Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods

Ramasamy¹,

Yi²,

An³

2015

IJN

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Escherichia coli O157:H7 (O157) is a Gram negative and highly virulent bacteria found in food and water sources, and is a leading cause of chronic diseases worldwide. Diagnosis and prevention from the infection require simple and rapid analysis methods for the detection of pathogens, including O157. Endogenous membrane peroxidase, an enzyme present on the surface of O157, was used for the colorimetric detection of bacteria by catalytic oxidation of the peroxidase substrate. In this study, we have analyzed the impact of the synthesized bare gold nanorods (AuNRs) and silica-coated AuNRs on the growth of E. coli O157. Along with the membrane peroxidase activity of O157, other bacteria strains were analyzed. Different concentrations of nanorods were used to analyze the growth responses, enzymatic changes, and morphological alterations of bacteria by measuring optical density, 3,3′,5,5′-tetramethylbenzidine assay, flow cytometry analysis, and microscopy studies. The results revealed that O157 showed higher and continuous membrane peroxidase activity than other bacteria. Furthermore, O157 treated with bare AuNRs showed a decreased growth rate in comparison with the bacteria with surface modified AuNRs. Interestingly, silica-coated AuNRs favored the growth of bacteria and also increased membrane peroxidase activity. This result can be particularly important for the enzymatic analysis of surface treated AuNRs in various microbiological applicants.

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Au@Pt nanostructures as oxidase and peroxidase mimetics for use in immunoassays

Cited by 532 publications

References 40 publications

Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity

Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity

Research progress of nanoparticles as enzyme mimetics

Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods

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