Au@organosilica multifunctional nanoparticles for the multimodal imaging

Abstract

The increasing application of nanomaterials in biosensor and imaging sets a higher demand on the multifunctionalities of nanomaterials for obtaining multiple parameters of a same system under a same condition. In this work, multifunctional Au@organosilica nanoparticles with high stability were conveniently synthesized by direct hydrolyzing of 3-mercaptopropyltriethoxysilane in an aqueous solution in the presence of a Au core. Modification of the Au core with Raman reporters and the organosilica shell with fluorophore before and after the hydrolysis, respectively, produces multifunctional nanoparticles exhibiting Rayleigh scattering of the Au core, fluorescence signals of the fluorophores and surface-enhanced Raman scattering (SERS) of the Raman reporters. The nanoparticles can be used as multimodal tracers for living cell imaging and related biological research.National Basic Research Program of China[2007CB935603, 2009CB930703, 2007DFC40440]; Natural Science Foundation of China[20620130427, 20825313, 20827003, 21021120456, 21021002

Cancer characterization and diagnosis with SERS-encoded particles

“…For instance, silica-or titania-coated SEPs (Fig. 2j) have been conjugated with fluorophores or quantum dots on the silica surface (Cui et al 2011;Qian et al 2011;Wang et al , 2014b. In this case, the final goal is combining the fast acquisition of fluorescence signal with the high multiplexing capability of SEPs.…”

“…With such complementary approaches, multimodal imaging technologies have been developed implementing SERS with other imaging techniques based on different physical effects such as fluorescence (Cui et al 2011;Qian et al 2011;Wang et al 2014b), magnetic resonance (Gao et al 2015;Ju et al 2015) and photoacoustics (Bao et al 2013;Chen et al 2016;Dinish et al 2015;Jokerst et al 2012a;Kircher et al 2012).…”

SERS Tags: Novel Optical Nanoprobes for Bioanalysis

“…248,264 Tian's group conjugated fluorescent molecules such as FITC to organosilica shells of the tags by simple mixing. 265 In another method, electrostatic force aids layer-by-layer deposition used for fluorescent decoration via the interaction between reporter-attached metal nanosubstrates and RBITC-labeled PAH 266,267 or fluorescent QDs. 96,268 RBITC was also reported to directly adsorb onto Ag on silica, polystyrene beads, 146 acting as both Raman reporter and fluorescence generator.…”

Biomedical applications of organosilica nanoparticles toward theranostics

“…Recently, internal functionalized organosilica nanoparticles with functional nanomaterials were synthesized and reported [67,153] . Gold nanoparticles encapsulated with a thiol -organosilica layer were prepared using MPMS as a single silica source in an aqueous solution.…”