Au decorated ZnO thin film: application to DNA sensing

Foo, K. L.; Hashim, U.; Voon, C. H.; Kashif, Muhammad; Ali, Eaqub

doi:10.1007/s00542-015-2572-x

Cited by 14 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 30 Foo et al described a Au-decorated ZnO thin-film-based biosensor using a thiol-modified single-stranded (ss) DNA probe. 31 However, all of these electrochemical methods involve an additional reducing/oxidizing agent, which makes the detection more complicated. In contrast, optical detection, namely, the fluorescence-based method, offers high sensitivity toward specific molecular recognition with a rapid response and easy operating technique.…”

Section: Introductionmentioning

confidence: 99%

“…Perumal et al developed a detection strategy for DNA from pathogenic leptospirosis with a gold-seeded ZnO nanoflower . Foo et al described a Au-decorated ZnO thin-film-based biosensor using a thiol-modified single-stranded (ss) DNA probe . However, all of these electrochemical methods involve an additional reducing/oxidizing agent, which makes the detection more complicated.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fluorescent ZnO–Au Nanocomposite as a Probe for Elucidating Specificity in DNA Interaction

et al. 2018

View full text Add to dashboard Cite

In this work, we report the interaction of a fluorescent ZnO–Au nanocomposite with deoxyribonucleic acid (DNA), leading to AT-specific DNA interaction, which is hitherto not known. For this study, three natural double-stranded (ds) DNAs having different AT:GC compositions were chosen and a ZnO–Au nanocomposite has been synthesized by anchoring a glutathione-protected gold nanocluster on the surface of egg-shell-membrane (ESM)-based ZnO nanoparticles. The ESM-based bare ZnO nanoparticles did not show any selective interaction toward DNA, whereas intrinsic fluorescence of the ZnO–Au nanocomposite shows an appreciable blue shift (Δλmax = 18 nm) in the luminescence wavelength of 520 nm in the presence of ds calf thymus (CT) DNA over other studied DNAs. In addition, the interaction of the nanocomposite through fluorescence studies with single-stranded (ss) CT DNA, synthetic polynucleotides, and nucleobases/nucleotides (adenine, thymine, deoxythymidine monophosphate, deoxyadenosine monophosphate) was also undertaken to delineate the specificity in interaction. A minor blue shift (Δλmax = 5 nm) in the emission wavelength at 520 nm was observed for single-stranded CT DNA, suggesting the proficiency of the nanocomposite for discriminating ss and ds CT DNA. More importantly, fluorescence signals from the nano-bio-interaction could be measured directly without any modification of the target, which is the foremost advantage emanated from this study compared with other previous reports. The AT base-pair-induced enhancement was also found to be highest for the melting temperature of CT DNA (ΔTmCT = 6.7 °C). Furthermore, spectropolarimetric experiments followed by calorimetric analysis provided evidence for specificity in AT-rich DNA interaction. This study would lead to establish the fluorescent ZnO–Au nanocomposite as a probe for nanomaterial-based DNA-binding study, featuring its specific interaction toward AT-rich DNA.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fluorescent ZnO–Au Nanocomposite as a Probe for Elucidating Specificity in DNA Interaction

et al. 2018

View full text Add to dashboard Cite

show abstract

“…This energy-level disparity is due to the work function of ZnO being higher (5.2–5.3 eV) than that of Au (5.1 eV), resulting in an electron transfer from Au to ZnO until the two systems reach dynamic equilibrium [ 97 , 98 ]. The electron transfer leads to an ohmic junction at the agglutination point due to a decrease in the ZnO depletion layer with a higher conductivity, which results in a decrease in the impedance, as shown in Fig 10A [ 19 , 99 ]. However, the opposite trend was observed for the 50 nm thick sputtered AuNP layer.…”

Section: Resultsmentioning

confidence: 99%

Thickness Dependent Nanostructural, Morphological, Optical and Impedometric Analyses of Zinc Oxide-Gold Hybrids: Nanoparticle to Thin Film

et al. 2015

View full text Add to dashboard Cite

The creation of an appropriate thin film is important for the development of novel sensing surfaces, which will ultimately enhance the properties and output of high-performance sensors. In this study, we have fabricated and characterized zinc oxide (ZnO) thin films on silicon substrates, which were hybridized with gold nanoparticles (AuNPs) to obtain ZnO-Aux (x = 10, 20, 30, 40 and 50 nm) hybrid structures with different thicknesses. Nanoscale imaging by field emission scanning electron microscopy revealed increasing film uniformity and coverage with the Au deposition thickness. Transmission electron microscopy analysis indicated that the AuNPs exhibit an increasing average diameter (5–10 nm). The face center cubic Au were found to co-exist with wurtzite ZnO nanostructure. Atomic force microscopy observations revealed that as the Au content increased, the overall crystallite size increased, which was supported by X-ray diffraction measurements. The structural characterizations indicated that the Au on the ZnO crystal lattice exists without any impurities in a preferred orientation (002). When the ZnO thickness increased from 10 to 40 nm, transmittance and an optical bandgap value decreased. Interestingly, with 50 nm thickness, the band gap value was increased, which might be due to the Burstein-Moss effect. Photoluminescence studies revealed that the overall structural defect (green emission) improved significantly as the Au deposition increased. The impedance measurements shows a decreasing value of impedance arc with increasing Au thicknesses (0 to 40 nm). In contrast, the 50 nm AuNP impedance arc shows an increased value compared to lower sputtering thicknesses, which indicated the presence of larger sized AuNPs that form a continuous film, and its ohmic characteristics changed to rectifying characteristics. This improved hybrid thin film (ZnO/Au) is suitable for a wide range of sensing applications.

show abstract

“…However, among various metal nanoparticles, noble gold (Au) nanoparticles have become the preferential choice for developing biosensors because Au nanoparticles (AuNP) are known to be a potential "bioconjugate" element. 19 Numerous reports have shown that the Au-doped ZnO thin film has produced an optimized nanocomposite, which possesses excellent electrical stability and sensitivity properties for gas sensing and bioapplications. Various doping methods of AuNP with ZnO have been mentioned in literature, such as spray-pyrosis, magnetron sputtering, vacuum evaporation system, and thermal oxidation process.…”

mentioning

confidence: 99%

Zinc oxide and gold textured Janus nanowire integration in an impedimetric sensor for leptospirosis DNA‐biomarker recognition

Haarindraprasad

Gopinath

Veeradassan

2022

Biotech and App Biochem

View full text Add to dashboard Cite

A "Janus particle" refers to the production of two materials in a single system and shows a difference in physical characteristics, and two surfaces participate in the formation with different chemistries. This research generated the Janus using a hybrid of zinc oxide (ZnO) and gold (Au) on the sensor surface toward making high-performance DNA sensors. The Janus ZnO/Au-textured film was synthesized via the one-step sol-gel method, which involves a suitable ratio of a mixture of ZnO sol seed solution. The synthesized Janus ZnO/Au-textured film undergoes a low-temperature aqueous hydrothermal route to synthesize quasione-dimensional nanowires. The average grain size in the Janus ZnO/Au nanotextured wire was 41.60 nm. The fabricated nanotextured wire was further optimized by tuning the thickness and characterized by XRD and high-resolution microscopy. Electrical characterization was conducted on the Janus ZnO/Au nanotextured wire coupled with an interdigitated electrode sensor to detect the specific leptospirosis DNA strand. The generated device is capable of detecting lower DNA concentration at 1 × 10 -13 M with a sensitivity of 8.54 MΩ M -1 cm -2 .The high performance is attained on linear concentrations of 10 -6 -10 -13 M with the determination coefficient, "I = 135437.63C−3609.07" R 2 = 0.9551. A potential strategy is proposed as a base for developing different high-performance sensors.

show abstract

Au decorated ZnO thin film: application to DNA sensing

Cited by 14 publications

References 32 publications

Fluorescent ZnO–Au Nanocomposite as a Probe for Elucidating Specificity in DNA Interaction

Fluorescent ZnO–Au Nanocomposite as a Probe for Elucidating Specificity in DNA Interaction

Thickness Dependent Nanostructural, Morphological, Optical and Impedometric Analyses of Zinc Oxide-Gold Hybrids: Nanoparticle to Thin Film

Zinc oxide and gold textured Janus nanowire integration in an impedimetric sensor for leptospirosis DNA‐biomarker recognition

Contact Info

Product

Resources

About