Au-courant and novel technologies for efficient doubled haploid development in barley (<i>Hordeum vulgare</i> L.)

Patial, Madhu; Chauhan, Ruchi; Chaudhary, H. K.; Pramanick, K. K.; Shukla, Arun Kumar; Kumar, Vikas; Verma, Ramesh Pal Singh

doi:10.1080/07388551.2022.2050181

Cited by 7 publications

(7 citation statements)

References 134 publications

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“…Doubled haploid (DH) technology is a valuable approach for accelerating genetic gain through a shortened breeding cycle. In wheat and barley, DHs obtained from immature microspores or in vitro ER following interspecific hybridization are important strategies for breeding and generating populations for gene mapping purposes (reviewed by Broughton et al [303]; Patial et al [304]).…”

Section: Monocot Speciesmentioning

confidence: 99%

“…The progressive elimination of H. bulbosum chromosomes also occurs in the endosperm, which prevents haploid embryo development. Therefore, haploid embryos must be rescued at 12-14 DAP by growing them in vitro before the degeneration of the endosperm [304]. Sanei et al [309] demonstrated that the loss of centromeric histone H3 (CENH3) from centromeres preceded uniparental chromosome deletion in interspecific barley hybrids (reviewed by Britt and Kuppu [310]).…”

Section: Monocot Speciesmentioning

confidence: 99%

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Embryo Rescue in Plant Breeding

Rogo,

Fambrini,

Pugliesi

2023

Plants

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Embryo rescue (ER) techniques are among the oldest and most successful in vitro tissue culture protocols used with plant species. ER refers to a series of methods that promote the development of an immature or lethal embryo into a viable plant. Intraspecific, interspecific, or intergeneric crosses allow the introgression of important alleles of agricultural interest from wild species, such as resistance or tolerance to abiotic and biotic stresses or morphological traits in crops. However, pre-zygotic and post-zygotic reproductive barriers often present challenges in achieving successful hybridization. Pre-zygotic barriers manifest as incompatibility reactions that hinder pollen germination, pollen tube growth, or penetration into the ovule occurring in various tissues, such as the stigma, style, or ovary. To overcome these barriers, several strategies are employed, including cut-style or graft-on-style techniques, the utilization of mixed pollen from distinct species, placenta pollination, and in vitro ovule pollination. On the other hand, post-zygotic barriers act at different tissues and stages ranging from early embryo development to the subsequent growth and reproduction of the offspring. Many crosses among different genera result in embryo abortion due to the failure of endosperm development. In such cases, ER techniques are needed to rescue these hybrids. ER holds great promise for not only facilitating successful crosses but also for obtaining haploids, doubled haploids, and manipulating the ploidy levels for chromosome engineering by monosomic and disomic addition as well substitution lines. Furthermore, ER can be used to shorten the reproductive cycle and for the propagation of rare plants. Additionally, it has been repeatedly used to study the stages of embryonic development, especially in embryo-lethal mutants. The most widely used ER procedure is the culture of immature embryos taken and placed directly on culture media. In certain cases, the in vitro culture of ovule, ovaries or placentas enables the successful development of young embryos from the zygote stage to maturity.

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Section: Monocot Speciesmentioning

confidence: 99%

Section: Monocot Speciesmentioning

confidence: 99%

Embryo Rescue in Plant Breeding

Rogo,

Fambrini,

Pugliesi

2023

Plants

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“…The genus Hordeum comprises about 32 species, including cultivated and wild ones for versatile uses (Visioni et al, 2023). The crop was mainly utilized for feed and food purposes but with shifting towards urbanization and adoption of western culture has led its utilization more for industrial purposes (Meints and Hayes, 2019, Kumar et al, 2023, Patial et al, 2023. Barley is also known as poor man's crop with low input requirement, well buffering capacity under harsh environmental conditions, salinity tolerance and with many positive health effects (Verma et al, 2016, Meng et al, 2023.…”

Section: Introductionmentioning

confidence: 99%

Heterosis and Diallel Analysis for Grain Yield, Yield Attributes and Physiological Traits in Barley (Hordeum vulgare L.)

Patel,

Kumar,

Saini

et al. 2024

IJBSM

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The present study was carried out during November–April, 2019–2020 and 2020–21 with eight diverse barley genotypes and their developed 28F1s (half diallel, excluding reciprocals) to ascertain gene effects, hetrosis and correlations for grain yield, yield attributes and physiological traits under water limited conditions of Bundelkhand region. Days to heading and days to maturity exhibited general mean values of 86 days and 127 days, respectively. The parental genotypes, DWRB180, RD2794 and RD2899 showed early spike emergence and maturity. The cross combinations, namely DWRB160 /DWRB180, DWRB180/RD2907 and BH946/RD2552 were promising for flag leaf length and width. The GCA effects were significant and prevalent for all the characters, except days to heading, plant height and tillers count. The parents, DWRB160 (5.29**) and BH902 (1.59**) depicted high GCA effects for 1000 grain weight. The parents, DWRB180, RD2552, and BH946 exhibited significant positive GCA effects for leaf area index and chlorophyll fluorescence, whereas the genotypes, RD2907, BH946 and RD2899 showed higher GCA effects for chlorophyll content. Grain yield exhibited significant positive correlations with flag leaf width (0.60**), tillers per meter (0.35*), grains per spike (0.39*), harvest index (0.53**), chlorophyll content (0.69**), leaf area index (0.49**) and chlorophyll fluorescence (0.68**). The parents, BH946, DWRB180 and RD2907 and the hybrids, RD2552/DWRB180, BH902/RD2794, DWRB160/DWRB180 and BH946/RD2907 were found promising for yield and yield attributes in the dry agro-ecology of Bundelkhand region. The crosses, DWRB160/BH902 and DWRB160/BH946 can be explored for developing superior segregants for malt barley breeding.

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“…In barley (Hordeum vulgare L.), due to the agronomic importance of the species, numerous protocols for plant regeneration via androgenesis have been described (Wrobel et al 2011;Castillo et al 2014;Makowska et al 2015;Patial et al 2022). Despite of this, there is still the need to optimize the culture conditions for higher efficiency of regeneration, and especially to minimize the production of albino plants (Makowska et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Gum Arabic influences the activity of antioxidant enzymes during androgenesis in barley anthers

Klajmon

Makowska

Zimny

et al. 2023

Plant Cell Tiss Organ Cult

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This study investigated the antioxidant activity of gum Arabic (GA) during androgenesis in barley anthers. After stress pretreatment the anthers were cultured in the presence of 10 mg L−1 GA (Gm) or on control medium (Cm) and compared with respect to activity of selected antioxidant and respiratory enzymes and endogenous hydrogen peroxide (H2O2) content. The anthers from Cm and Gm differed in the strategy against H2O2 overproduction, in that the total peroxidase (POX) activity and the number of POX isoforms were significantly higher in Gm- than in Cm-cultured material. High POX activity on Gm paralleled with H2O2 decrease, suggesting the utilization of this chemical for the POX-mediated cell wall formation and reconstruction during growth of multicellular structures. The total superoxide dismutase (SOD) activity on Cm and Gm were at similar level for most of the culture period but the activity of MnSOD was dozen times higher on Gm and this coincided with high activity of fumarase and cytochrome c oxidase. It indicates close interplay between efficient antioxidative protection and high metabolic rate accompanying efficient androgenesis. Mass spectrometry analysis confirmed the presence of POX and other antioxidative and defense enzymes in protein fraction of GA used in the experiments, however as revealed by 2,2-diphenyl-1-picrylhydrazyl assay, the Gm and Cm displayed similar total antioxidant capacity. Thus, the effect of GA on androgenic cultures of barley can be linked to its influence on the activity of anther antioxidant system, rather than its native antioxidative properties.

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Au-courant and novel technologies for efficient doubled haploid development in barley (Hordeum vulgare L.)

Cited by 7 publications

References 134 publications

Embryo Rescue in Plant Breeding

Embryo Rescue in Plant Breeding

Heterosis and Diallel Analysis for Grain Yield, Yield Attributes and Physiological Traits in Barley (Hordeum vulgare L.)

Gum Arabic influences the activity of antioxidant enzymes during androgenesis in barley anthers

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