Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging

Han, Song; Mu, Ying; Zhu, Qiangyuan; Gao, Yibo; Li, Zuhong; Jin, Qinhan; Jin, Wei

doi:10.1007/s00216-012-5921-y

Cited by 19 publications

(15 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detailed histological examination of the tumor tissue revealed that targeting was vascular integrin α V β 3 specific with little extravasation. Over the last several years, many studies from other research groups have also demonstrated that RGD peptide-conjugated QDs could exhibit highly specific tumor targeting and reduced accumulation in the lung, kidney, and heart in mice models [86, 137-140]. …”

Section: In Vivo Targeted Imaging With Qdsmentioning

confidence: 99%

Quantum Dot-Based Nanoprobes for In Vivo Targeted Imaging

Zhu¹,

Hong²,

Xu³

et al. 2013

CMM

View full text Add to dashboard Cite

Fluorescent semiconductor quantum dots (QDs) have attracted tremendous attention over the last decade. The superior optical properties of QDs over conventional organic dyes make them attractive labels for a wide variety of biomedical applications, whereas their potential toxicity and instability in biological environment has puzzled scientific researchers. Much research effort has been devoted to surface modification and functionalization of QDs to make them versatile probes for biomedical applications, and significant progress has been made over the last several years. This review article aims to describe the current state-of-the-art of the synthesis, modification, bioconjugation, and applications of QDs for in vivo targeted imaging. In addition, QD-based multifunctional nanoprobes are also summarized.

show abstract

Section: In Vivo Targeted Imaging With Qdsmentioning

confidence: 99%

Quantum Dot-Based Nanoprobes for In Vivo Targeted Imaging

Zhu¹,

Hong²,

Xu³

et al. 2013

CMM

View full text Add to dashboard Cite

show abstract

“…Regarding CEA expression [136], specific probes were developed to detect in vivo this oncofetal protein, by labeling specific monoclonal antibodies with Cy5.5 [137], Alexafluor488 [138], fluorescent cyanine DY-676 [139], QD820 [140], or bioluminescent proteins such as Renilla Luciferase [141] and Gaussia Luciferase [142]. Even if Renilla and Gaussia Luciferases are not fluorescent probes, this is an alternative strategy that can be used to target CEA by Optical imaging using Bioluminescence.…”

Section: Tumor Cell Biomarkersmentioning

confidence: 99%

“…Cetuximab (or Erbitux, a monoclonal antibody already used in cancer therapy) has been labeled with Cy5.5 [154–156], QD840 [140] or Bodipy-FL [157] (a fluorophore that emits at 513 nm). Cy5.5-Cetuximab [156] has been injected in mice bearing MCF-7 and MDA-MB-231 breast cancers, and the tumor localization was monitored by fluorescence imaging.…”

Section: Tumor Cell Biomarkersmentioning

confidence: 99%

“…Several examples have been described: lung carcinoma bearing mice were co-injected with QD800-RGD, QD820-antiCEA and QD840-antiEGFR [140] and MultiSpectral Fluorescence Imaging (MSFI) allowed to differentially resolve the information given by the three probes. A fluorescent signal was observed for all the probes in primary and in metastatic lesions, as well as in lymphatic basin, as expected, since lung cancer can spread locally near the tumor or in the regional lymph nodes.…”

Section: Tumor Cell Biomarkersmentioning

confidence: 99%

See 1 more Smart Citation

Optical imaging probes in oncology

et al. 2016

View full text Add to dashboard Cite

Cancer is a complex disease, characterized by alteration of different physiological molecular processes and cellular features. Keeping this in mind, the possibility of early identification and detection of specific tumor biomarkers by non-invasive approaches could improve early diagnosis and patient management.Different molecular imaging procedures provide powerful tools for detection and non-invasive characterization of oncological lesions. Clinical studies are mainly based on the use of computed tomography, nuclear-based imaging techniques and magnetic resonance imaging. Preclinical imaging in small animal models entails the use of dedicated instruments, and beyond the already cited imaging techniques, it includes also optical imaging studies. Optical imaging strategies are based on the use of luminescent or fluorescent reporter genes or injectable fluorescent or luminescent probes that provide the possibility to study tumor features even by means of fluorescence and luminescence imaging. Currently, most of these probes are used only in animal models, but the possibility of applying some of them also in the clinics is under evaluation.The importance of tumor imaging, the ease of use of optical imaging instruments, the commercial availability of a wide range of probes as well as the continuous description of newly developed probes, demonstrate the significance of these applications. The aim of this review is providing a complete description of the possible optical imaging procedures available for the non-invasive assessment of tumor features in oncological murine models. In particular, the characteristics of both commercially available and newly developed probes will be outlined and discussed.

show abstract

“…10 So far, many near-infrared range (NIR) fluorescent probes have been developed (such as quantum dots) for in-vivo imaging studies. [11][12][13][14][15] A single-view two-dimensional (2-D) imaging system is the mainstream of in-vivo fluorescence imaging equipment. [16][17][18][19] However, they cannot meet the needs for accurate positioning during the studies.…”

Section: Introductionmentioning

confidence: 99%

In vivonear-infrared fluorescence three-dimensional positioning system with binocular stereovision

et al. 2014

Self Cite

View full text Add to dashboard Cite

Abstract. Fluorescence is a powerful tool for in-vivo imaging in living animals. The traditional in-vivo fluorescence imaging equipment is based on single-view two-dimensional imaging systems. However, they cannot meet the needs for accurate positioning during modern scientific research. A near-infrared in-vivo fluorescence imaging system is demonstrated, which has the capability of deep source signal detecting and three-dimensional positioning. A three-dimensional coordinates computing (TDCP) method including a preprocess algorithm is presented based on binocular stereo vision theory, to figure out the solution for diffusive nature of light in tissue and the emission spectra overlap of fluorescent labels. This algorithm is validated to be efficient to extract targets from multispectral images and determine the spot center of biological interests. Further data analysis indicates that this TDCP method could be used in three-dimensional positioning of the fluorescent target in small animals. The study also suggests that the combination of a large power laser and deep cooling charge-coupled device will provide an attractive approach for fluorescent detection from deep sources. This work demonstrates the potential of binocular stereo vision theory for three-dimensional positioning for living animal in-vivo imaging. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Au:CdHgTe quantum dots for in vivo tumor-targeted multispectral fluorescence imaging

Cited by 19 publications

References 68 publications

Quantum Dot-Based Nanoprobes for In Vivo Targeted Imaging

Quantum Dot-Based Nanoprobes for In Vivo Targeted Imaging

Optical imaging probes in oncology

In vivonear-infrared fluorescence three-dimensional positioning system with binocular stereovision

Contact Info

Product

Resources

About