Atypical Spitzoid melanocytic tumors: A morphological, mutational, and FISH analysis

Massi, Daniela; Cesinaro, Anna Maria; Tomasini, Carlo Francesco; Paglierani, Milena; Bettelli, Stefania; Maso, Luigino Dal; Simi, Lisa; Salvianti, Francesca; Pinzani, Pamela; Orlando, Claudio; Giorgi, Vincenzo De; Lukic, Silvana; Maiorana, Antonio; Santucci, Marco; Canzonieri, Vincenzo

doi:10.1016/j.jaad.2010.05.043

Cited by 66 publications

(59 citation statements)

References 45 publications

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“…We pointed out that this subgroup of tumors has a much more favorable prognosis than ''conventional'' melanomas and that the well-known, important prognosticators for conventional melanomas, such as tumor thickness and sentinel lymph node tumor-harboring status, do not appear to have the same significance for them. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] These facts support our proposal that such melanocytic tumors are biologically distinct and should be classified in a nosologic category separate from both melanomas and nevi.…”

supporting

confidence: 70%

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Scolyer

Zembowicz

2012

Archives of Pathology & Laboratory Medicine

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supporting

confidence: 70%

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Scolyer

Zembowicz

2012

Archives of Pathology & Laboratory Medicine

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“…Early retrospective studies showed correlation between metastatic behavior among histologically ambiguous melanocytic lesions and FISH results. 5,14,20 However, applying data generated from FISH validation studies to routine diagnosis of challenging lesions is not straightforward. By definition, there are no ''teaching sets'' of ambiguous melanocytic lesions.…”

Section: Discussionmentioning

confidence: 99%

Correlation Between Histologic Assessment and Fluorescence In Situ Hybridization Using MelanoSITE in Evaluation of Histologically Ambiguous Melanocytic Lesions

Zembowicz

Yang²,

Kafanas

et al. 2012

Archives of Pathology & Laboratory Medicine

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N Context.-The 4-probe, multicolor, fluorescence in situ hybridization (FISH) panel targeting chromosomes 6 and 11 has shown promising sensitivity and specificity in distinguishing between benign nevi and malignant melanoma. Only a few studies have assessed the potential utility of FISH in classification of histologically ambiguous melanocytic lesions. In the United States, this assay is exclusively licensed to NeoGenomics Laboratories (Irvine, California), which provides the technical component and has developed an innovative service (MelanoSITE) allowing pathologists to interpret FISH results using a dedicated Web portal. Thus far, use of MelanoSITE as a diagnostic adjunct in the diagnosis of melanocytic lesions has not, to our knowledge, been reported in the literature.Objective.-To analyze 1.5 years of experience with the MelanoSITE melanoma FISH assay in the evaluation of histologically ambiguous lesions in the context of second opinion and routine dermatopathology practice.Design.-A prospective histologic/FISH correlation study of 140 cases.Results.-Twenty-seven percent of abnormal FISH results were false-positive results because of tetraploidy. After correcting for known false-positive results, all lesions considered atypical nevi showed normal FISH signals. Abnormal FISH signals were reported in 30% of lesions considered histologically borderline and in 48% of lesions in which a diagnosis of melanoma was favored.Conclusions.-Four-probe, multicolor FISH results for melanoma correlate with the microscopic assessments of histologically ambiguous lesions. Pathologists using MelanoSITE must be aware of the high rate of false-positive results from tetraploidy.

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“…Ras responsive element binding protein 1 (RREB1) is an important transcription factor, which can initiate the transcription of many downstream genes once activated. It has been reported that RREB1 is associated with type 2 diabetes and tumor (16,17). However, the expression pattern and role of RREB1 in neuropathic pain remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Role of miR-145 in chronic constriction injury in rats

Pang

Tang

Zhang

2016

Experimental and Therapeutic Medicine

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Abstract. The present study aims to investigate the effects and underlying mechanisms of miRNA-145 (miR-145) in rat models of chronic constriction injury (CCI). Rats were randomly divided into control, sham, CCI, agomiRNA (agomiR)-normal control (NC) and agomiR-145 groups (n=25 in each group); in addition, 30 rats with CCI were divided into small hairpin (sh)RNA-NC and shRNA-ras responsive element binding protein 1 (RREB1) groups. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) were detected. Reverse transcription-quantitative polymerase chain reaction was used to detect miR-145 expression levels, and western blotting was performed to measure RREB1 and phosphorylated-protein kinase B (p-AKT) expression levels. In addition, a dual luciferase reporter assay was conducted to identify the target gene of miR-145. PWMT and PWTL were decreased in CCI rats and this decrease was alleviated by miR-145 injection. At 1, 3, 5 and 7 days after CCI, miR-145 expression level in the spinal cord tissue of rats in the CCI group was significantly decreased compared with 1 day before CCI (P<0.05). Compared with the CCI group, miR-145 expression level in the agomiR-145 group was significantly higher (P<0.05). In addition, expression levels of RREB1 and p-AKT were significantly increased in the CCI group and significantly decreased in the agomiR-145 group (P<0.05). Furthermore, knockdown of RREB1 expression by shRNA-RREB1 significantly increased values of PWMT and PWTL, decreased expression levels of RREB1 and p-AKT, and increased miR-145 expression levels (P<0.05). Further investigation demonstrated that miR-145 can bind with RREB1 mRNA. In conclusion, miR-145 may be involved in the development of CCI through regulating the expression of RREB1.

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Atypical Spitzoid melanocytic tumors: A morphological, mutational, and FISH analysis

Cited by 66 publications

References 45 publications

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Correlation Between Histologic Assessment and Fluorescence In Situ Hybridization Using MelanoSITE in Evaluation of Histologically Ambiguous Melanocytic Lesions

Role of miR-145 in chronic constriction injury in rats

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