Atypical reticulocytes in rats with malaria as a possible consequence of the pitting function of the spleen

Tosta, Carlos Eduardo; Hermans, Maria A.

doi:10.1080/00034983.1981.11687452

Cited by 2 publications

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“…In conclusion, in vitro data from this study indicate that pitting of malaria parasites can produce spherocytes. Taken together with other studies that indicate that pitting of malaria parasites is common [ 18 , 19 ], and indeed preferred to phagocytosis of infected RBC [ 16 ], it is proposed that, in malaria holoendemic areas where the prevalence of asexual stage malaria parasites approaches 100% in children [ 1 ], the day-in-day-out pitting of malaria parasites produces high spherocytic rates. Of course, spherocyte formation may be a consequence of the pitting of intraerythrocytic inclusion bodies like Howell-Jolly bodies or the Heinz bodies produced in conditions associated with RBC oxidant stress such as glucose-6-phosphate dehydrogenase (G6PD) deficiency.…”

Section: Discussionmentioning

confidence: 91%

Pitting of malaria parasites and spherocyte formation

Anyona

Schrier

Gichuki

et al. 2006

Malar J

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Background: A high prevalence of spherocytes was detected in blood smears of children enrolled in a case control study conducted in the malaria holoendemic Lake Victoria basin. It was speculated that the spherocytes reflect intraerythrocytic removal of malarial parasites with a concurrent removal of RBC membrane through a process analogous to pitting of intraerythrocytic inclusion bodies. Pitting and re-circulation of RBCs devoid of malaria parasites could be a host mechanism for parasite clearance while minimizing the anaemia that would occur were the entire parasitized RBC removed. The prior demonstration of RBCs containing ring-infected erythrocyte surface antigen (pf 155 or RESA) but no intracellular parasites, support the idea of pitting.

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Section: Discussionmentioning

confidence: 91%

Pitting of malaria parasites and spherocyte formation

Anyona

Schrier

Gichuki

et al. 2006

Malar J

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The role of spleen macrophages in malaria: an ultrastructural study

Tosta

Ruiz

Wedderburn

1984

Rev. Soc. Bras. Med. Trop.

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A n electronmicroscopy study o f the spleen fro m mice infected withThe predominantly intraerythrocytic habitat of malarial parasites and the fact that gross changes in the splenic microarchitecture occur during m alaria8 , make the spleen an im portant organ in which to study host -parasite interactions. A t this site parasites are removed from the blood, phagocytosed, and both induce and are subjected to the effects of the immune response. Although macrophages play a central role in ali these events, our understanding of their functions is still very fragmentary and several important questions remain unanswered. This study was therefore undertaken to investigate the nature of phagocytic cells from different areas of the spleen during malaria infection, and the mechanisms by which they remove parasites from the blood. strain) -infected erythrocytes, and eight days later, anesthetized by ether 30 minutes after an injection of 150 units of heparin intravenously. A polythene cannula (0.61 mm externai diameter) was inserted caudally into the thoracic aorta and its tip secured at the levei of emergence of the coeliac artery. After clamping the distai aorta and sectioning the portal vein, animais were perfused with phosphate buffered saline at 37°C at a pressure of 163 cm of water. After bleaching of the liver, perfusion was switched to the fixation solution of 1.5% glutaraldehyde in 0.1M sodium cacodylate bufferpH 7.3. Spleens were remov ed after 15 min perfusion with the fixative, sliced into sections of 1 mm submitted to a further 2 hour fixation with 4% buffered glutaraldehyde and washed with cacodylate buffer containing 10% sucrose. Tissues were post fixed in veronal-acetate buffered 1 % osmium tetroxide, dehydrated in ethanol, embedded in Araldite and stained with uranyl acetate and lead citrate, and examined by electron microscopy.A reas comprising each major cell compartment of the spleen (red pulp, marginal zone, periarteriolar lymphocyte sheath and germinal centre) were selected in toluidine blue-stained sections, and relocated on the surface of the corresponding block. Phagocytic cells were characterized by the presence of ingested parasites or malarial pigment. The latter was recognized as osmiophilic trapezoidal crystals, sometimes partially dissolved by the lead citrate used for processing the tissue. 31

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Atypical reticulocytes in rats with malaria as a possible consequence of the pitting function of the spleen

Cited by 2 publications

References 0 publications

Pitting of malaria parasites and spherocyte formation

Pitting of malaria parasites and spherocyte formation

The role of spleen macrophages in malaria: an ultrastructural study

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