Atypical laccase isoenzymes from copper supplemented Pleurotus ostreatus cultures

Palmieri, Gianna; Cennamo, G.; Faraco, Vincenza; Amoresano, Angela; Sannia, Giovanni; Giardina, Paola

doi:10.1016/s0141-0229(03)00117-0

Cited by 133 publications

(107 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The concomitant occurrence of different laccase forms as observed (Fig. 6) is a well-known phenomenon in filamentous fungi and may be attributed to multiple laccase genes or post-translational modification (Litvintseva & Henson, 2002;Palmieri et al, 2003;Thurston, 1994). C. aquatica has been connected to ascomycetous teleomorphs (Nikolcheva & Bärlocher, 2004;Webster, 1992).…”

Section: Discussionmentioning

confidence: 91%

Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases

et al. 2005

View full text Add to dashboard Cite

Degradation of technical nonylphenol (t-NP), known as an endocrine-disrupting compound mixture, was assessed, using the mitosporic fungal strain UHH 1-6-18-4 isolated from nonylphenol-contaminated river water, and a strain of the aquatic hyphomycete Clavariopsis aquatica. GC-MS analysis could resolve 12 peaks attributable to nonyl chain-branched t-NP isomers. All were degraded, to individual extents. Analysis of degradation metabolites suggested intracellular hydroxylation of the nonyl moieties of individual t-NP isomers. Further metabolites also indicated shortening of branched nonyl chains, and 4-hydroxybenzoic acid was identified as a t-NP breakdown product in UHH 1-6-18-4. The t-NP degradation efficiency was higher in UHH 1-6-18-4 than in C. aquatica, and a lower specificity in degradation of individual t-NP constituents in UHH 1-6-18-4 than in C. aquatica was observed. Strain UHH 1-6-18-4 concomitantly produced extracellular laccase under degradation conditions. A mixture of CuSO 4 and vanillic acid considerably enhanced laccase production in both fungi. Laccase preparations derived from UHH 1-6-18-4 and C. aquatica cultures also converted t-NP. Laccase-catalysed transformation of t-NP led to the formation of products with higher molecular masses than that of the parent compound. These results emphasize a role of fungi occurring in aquatic ecosystems in degradation of water contaminants with endocrine activity, which has not previously been considered. Furthermore, the results are in support of two different mechanisms employed by fungi isolated from aquatic environments to initiate t-NP degradation: hydroxylation of individual t-NP isomers at their branched nonyl chains and further breakdown of the alkyl chains of certain isomers; and attack of t-NP by extracellular laccase, the latter leading to oxidative coupling of primary radical products to compounds with higher molecular masses. INTRODUCTIONNonylphenols have increasingly gained attention because of their potential to mimic the action of natural hormones in vertebrates (Ying et al., 2002). They result from incomplete biodegradation of nonylphenol polyethoxylates (NPEOs), which have been widely used as non-ionic surfactants in industrial processes and households (Braun et al., 2003;Ying et al., 2002). Both nonylphenols and NPEOs are discharged into the environment, mainly due to incomplete removal in wastewater treatment facilities (Ying et al., 2002). Nonylphenols are more resistant to biodegradation than their parent compounds and hence are found worldwide in wastewater treatment plant effluents and rivers in concentrations of up to the mg l 21 range (Heemken et al., 2001;Kolpin et al., 2002;Stachel et al., 2003;Ying et al., 2002). Due to their hydrophobicity, they tend to adsorb onto surface water particles and sediments and accumulate in aquatic organisms (Heemken et al., 2001;Ying et al., 2002). Consequently, nonylphenols represent a serious environmental and human health risk. The assessment of biodegradative processes affecting the environmental...

show abstract

Section: Discussionmentioning

confidence: 91%

Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Six appear clustered at the subtelomere region of chromosome VI, and the others map to chromosomes IV, VI, VII, VIII, and XI (two genes) (33). However, only six P. ostreatus laccase isozymes, POXA1b (Lacc6), POXA1w, POXA2, POXA3a (Lacc2), POXA3b, and POXC (Lacc10), have been biochemically characterized to date (10,11,32). Among them, Lacc2, Lacc6, and Lacc10 are the most studied, and their genes have been expressed heterologously in yeast (8,40).…”

mentioning

confidence: 99%

Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures

Castanera

Pérez

Omarini

et al. 2012

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

ABSTRACTThe genome of the white rot basidiomycetePleurotus ostreatusincludes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription-quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) theLacc2andLacc10genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and theLaccgene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors.

show abstract

“…(Kunamneni et al, 2007). Laccase 생산은 백색부후균이나 호지성기생균, 병원성 곰팡이뿐만 아니라 식용버섯인 양송이버섯 (Perry et al, 1993), 느타 리버섯 (Giardina et al, 1999;Palmieri et al, 1997;Palmieri et al, 2003), 표고버섯 등에도 알려져 있다. 느 타리버섯은 8개의 다른 laccase isozymes이 알려졌다 (Gianfreda et al, 1999;Palmieri et al, 1997;Palmieri et al, 2003).…”

Section: Xylanaseunclassified

Industrial applications and characteristics of lignocellulolytic enzymes in Basidiomycetous fungi

Lim¹,

Kang²

2016

Journal of Mushroom

View full text Add to dashboard Cite

Atypical laccase isoenzymes from copper supplemented Pleurotus ostreatus cultures

Cited by 133 publications

References 30 publications

Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases

Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases

Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures

Industrial applications and characteristics of lignocellulolytic enzymes in Basidiomycetous fungi

Contact Info

Product

Resources

About