Atypical
            <i>cpb2</i>
            Genes, Encoding Beta2-Toxin in
            <i>Clostridium perfringens</i>
            Isolates of Nonporcine Origin

Jost, B. Helen; Billington, Stephen J.; Trinh, Hien T.; Bueschel, Dawn M.; Songer, J. Glenn

doi:10.1128/iai.73.1.652-656.2005

Cited by 69 publications

(102 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recent genotypic studies have provided mixed support for the importance of cpb2 in poultry enteric disease (15,42). Overall, cpb2 was present in 78.2% of all isolates surveyed; however, the presence of this gene was significantly correlated with C. perfringens isolates recovered in cases of NE (P, 0.007) but not PG (P, 0.093).…”

Section: Discussionmentioning

confidence: 83%

See 1 more Smart Citation

Multilocus Sequence Typing Subtypes of Poultry Clostridium perfringens Isolates Demonstrate Disease Niche Partitioning

Hibberd¹,

Neumann²,

Rehberger³

et al. 2011

J Clin Microbiol

View full text Add to dashboard Cite

Clostridium perfringens is a ubiquitous and versatile pathogenic bacterium and is implicated in the etiology of the poultry diseases necrotic enteritis (NE) and poultry gangrene (PG). In this study, multilocus sequence typing was used to investigate genotypic relationships among 139 C. perfringens isolates from 74 flocks. These isolates had multiple disease, host, and environmental origins. The results indicated a polymorphic yet highly clonal population, with 79.6% of all isolates partitioning into one of six clonal complexes or two dominant sequence types, ST-9 and ST-31. The most prolific clonal complex, CC-1, contained 27.3% of all isolates and was not clearly associated with one particular disease. The subtypes CC-4 and ST-31 were highly associated with NE and represented 9.4% and 7.2% of the total isolates, respectively. No PG-associated and NE-associated C. perfringens isolates shared the same sequence type or clonal complex. NE-associated subtypes were more clonal and appeared more evolutionarily divergent than PG-associated subtypes, which tended to cluster in the more ancestral lineages alongside isolates from asymptomatic chickens and turkeys. Toxin gene screening identified cpb2 throughout these isolates and correlated the presence of netB with NE pathology. Previous investigations into the genetic basis of C. perfringens pathogenicity have focused on toxins and other variable genetic elements. This study presents the first sequence-based comparison of C. perfringens isolates recovered in clinical cases of PG and NE and demonstrates that niche specialization is observable in the core genomes of poultry-associated C. perfringens isolates, a concept with both epidemiological and evolutionary significance.

show abstract

Section: Discussionmentioning

confidence: 83%

“…NE-associated subtypes contained comparatively high numbers of netB-positive isolates; netB was absent in all PGassociated subtypes and present in 18.5% of non-diseaseassociated isolates. Further investigation of cpb2 and netB toxin expression may support phylogenetic disease associations in vivo (15,19).…”

Section: Discussionmentioning

confidence: 99%

Multilocus Sequence Typing Subtypes of Poultry Clostridium perfringens Isolates Demonstrate Disease Niche Partitioning

Hibberd¹,

Neumann²,

Rehberger³

et al. 2011

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…The original cpb2 was termed the consensus gene/allele whereas the variant was denominated the atypical gene or atypical allele (11,12). The consensus cpb2 allele has been identified in porcine isolates of C. perfringens, while the atypical allele was present predominantly in isolates from nonporcine hosts (11,13). The cpb2 gene has been found in isolates originating from humans and a variety of other animals.…”

Section: Introductionmentioning

confidence: 99%

“…The cpb2 gene was shown to be located on a plasmid (1) and two alleles of the cpb2 gene were described (10,11). The original cpb2 was termed the consensus gene/allele whereas the variant was denominated the atypical gene or atypical allele (11,12). The consensus cpb2 allele has been identified in porcine isolates of C. perfringens, while the atypical allele was present predominantly in isolates from nonporcine hosts (11,13).…”

Section: Introductionmentioning

confidence: 99%

“…A possible pore formation or other mechanisms leading to cell membrane disruption appear to be its most plausible function (1). The cpb2 gene was shown to be located on a plasmid (1) and two alleles of the cpb2 gene were described (10,11). The original cpb2 was termed the consensus gene/allele whereas the variant was denominated the atypical gene or atypical allele (11,12).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens

Tolooe¹,

Shojadoost²,

Peighambari³

2011

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Abstract:Clostridium perfringens is an important pathogen in both human and veterinary medicine. Necrotic enteritis (NE) is the most clinically dramatic bacterial enteric disease of poultry induced by C. perfringens. The pathogenicity of this bacterium is associated with the production of extracellular toxins produced by some of its strains, such as beta2 toxin. The exact role of beta2 toxin in NE pathogenesis is still controversial. In the present study, C. perfringens isolates from healthy and diseased poultry flocks from different parts of Iran were analyzed by PCR assay to determine the presence of all variants of the beta2 toxin gene (cpb2). The products of two positive cpb2 PCR reactions were sequenced, compared to each other and to the cpb2 sequences published in GenBank (by multiple alignment and phylogenetic analysis). The current work represents the first study of cpb2 in poultry C. perfringens isolates in Asia, and reports the highest percentage of cpb2-positive isolates in both apparently healthy chickens (97.7%) and those afflicted with NE (94.4 %). The sequenced isolates were classified as atypical. This study did not show a direct correlation between NE occurrence and cpb2 presence.

show abstract

Clostridium perfringens Beta2 toxin forms highly cation-selective channels in lipid bilayers

et al. 2021

View full text Add to dashboard Cite

Clostridium perfringens is a potent producer of a variety of toxins. Well studied from these are five toxins (alpha, Beta (CPB), epsilon, iota and CPE) that are produced by seven toxinotype strains (A–G) of C. perfringens. Besides these toxins, C. perfringens produces also another toxin that causes necrotizing enterocolitis in piglets. This toxin termed consensus Beta2 toxin (cCPB2) has a molecular mass of 27,620 Da and shows only little homology to CPB and no one to the other toxins of C. perfringens. Its primary action on cells remained unknown to date. cCPB2 was heterogeneously expressed as fusion protein with GST in Escherichia coli and purified to homogeneity. Although cCPB2 does not exhibit the typical structure of beta-stranded pore-forming proteins and contains no indication for the presence of amphipathic alpha-helices we could demonstrate that cCPB2 is a pore-forming component with an extremely high activity in lipid bilayers. The channels have a single-channel conductance of about 700 pS in 1 M KCl and are highly cation-selective as judged from selectivity measurements in the presence of salt gradients. The high cation selectivity is caused by the presence of net negative charges in or near the channel that allowed an estimate of the channel size being about 1.4 nm wide. Our measurements suggest that the primary effect of cCPB2 is the formation of cation-selective channels followed by necrotic enteritis in humans and animals. We searched in databases for homologs of cCPB2 and constructed a cladogram representing the phylogenetic relationship to the next relatives of cCPB2.

show abstract

Atypical cpb2 Genes, Encoding Beta2-Toxin in Clostridium perfringens Isolates of Nonporcine Origin

Cited by 69 publications

References 14 publications

Multilocus Sequence Typing Subtypes of Poultry Clostridium perfringens Isolates Demonstrate Disease Niche Partitioning

Multilocus Sequence Typing Subtypes of Poultry Clostridium perfringens Isolates Demonstrate Disease Niche Partitioning

Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens

Clostridium perfringens Beta2 toxin forms highly cation-selective channels in lipid bilayers

Contact Info

Product

Resources

About