Atypical human rotaviruses in Hungary

Szücs, György; Kende, Meir; M, Uj

doi:10.1016/s0769-2617(87)80026-6

Cited by 10 publications

(12 citation statements)

References 13 publications

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“…Rotaviruses group C were first detected from an Australian infant hospitalized with diarrhea in 1973 (Rodger et al 1982). Since then, rotaviruses group C have been recognized as a cause of gastroenteritis in all age groups, accounting for either sporadic cases or large outbreaks of gastroenteritis in closed and semi-closed communities (Szucs et al 1987;Saif and Jiang 1994;Jiang et al 1995;Gabbay et al 1999;Sánchez-Fauquier et al 2003;Phan et al 2004;Schnagl et al 2004;Kuzuya et al 2005). The observed differences in the epidemiological features for different groups of rotaviruses may be accounted by different mechanisms of transmission or by their different stabilities in the environment and their overall ecology.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Rotaviruses Groups A and C in Egyptian Children and Aquatic Environment

2015

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The objective of this study is to compare the prevalence of rotaviruses groups A and C in Egyptian children and aquatic environment. From 110 stool specimens of children with acute diarrhea and using RT-PCR, 35 samples (31.8 %) were positive for human rotavirus group A and 15 samples (13.6 %) were positive for human rotavirus group C. From 96 samples collected from Zenin wastewater treatment plant over a 2-year period (November 2009-October 2011) and using RT-PCR, rotavirus group A was detected in (4/24) 16.7 %, (5/24) 20.8 %, (4/24) 16.7 %, and (4/24) 16.7 %, while rotavirus group C was detected in (2/24) 8.3 %, (3/24) 12.5 %, (3/24) 12.5 %, and (0/24) 0 % in raw sewage, after primary sedimentation, after secondary sedimentation, and after final chlorination, respectively. Moreover, from 96 samples collected from El-Giza water treatment plant over a 2-year period (November 2009-October 2011), rotavirus group A was detected in (7/24) 29.2 %, (6/24) 25 %, (5/24) 20.8 %, and (3/24) 12.5 %, while rotavirus group C was detected in (3/24) 12.5 %, (1/24) 4.2 %, (1/24) 4.2 %, and (0/24) 0 % in raw Nile water, after sedimentation, after sand filtration, and after final chlorination, respectively. Using SYBR Green real-time RT-PCR, the number of human rotavirus group A genome or infectious units was higher than rotavirus group C. VP6 sequence analysis of the RT-PCR positive rotavirus group C samples revealed that four clinical specimens and three environmental samples showed similar sequences clustered with Moduganari/Human Nigerian strain AF 325806 with 98 % homology, and two clinical specimens and one environmental sample showed similar sequences clustered with Dhaka CB/Human Bangladesh strain AY 754826 with 97 % homology.

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Section: Introductionmentioning

confidence: 99%

Prevalence of Rotaviruses Groups A and C in Egyptian Children and Aquatic Environment

2015

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“…9 Group C rotaviruses (GCRVs) were first recognized as a causative agent of gastroenteritis in pigs in the 1980s 3 and were subsequently identified as an emerging human pathogen. 11,14,16,17 Epidemiologic studies suggest that porcine GCRVs are widespread in pig herds and are presumably zoonotic. 18 However, the precise prevalence of porcine GCRVs remains largely unknown because of the absence of reliable, specific, and rapid diagnostic methods.…”

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confidence: 99%

Development of One-Step Real-Time Reverse Transcription Polymerase Chain Reaction Assays for Rapid Detection of Porcine Group C Rotaviruses

et al. 2010

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Abstract. Although the widespread occurrence of porcine group C rotaviruses (GCRV) is assumed, precise prevalence remains largely unknown because of the absence of reliable, specific, and rapid diagnostic methods. To detect and quantify porcine GCRV, the authors evaluated and optimized SYBR Green and TaqMan real-time reverse transcription polymerase chain reaction (RT-PCR) assays and applied them to 108 piglet fecal samples. Using serially diluted standard RNA transcripts of porcine GCRV VP6 gene, both SYBR Green and TaqMan real-time RT-PCR assays detected as few as 1 3 10 1 genome copies/ml (correlation coefficiency .0.99), whereas conventional RT-PCR detected 1.0 3 10 3 copies/ml. In addition, the conventional assay detected porcine GCRV in 24% (26/108) of fecal samples, whereas the detection rates of both SYBR Green and TaqMan assays were 72% (78 of 108) and 64% (70 of 108), respectively. The current study indicated that both real-time RT-PCR assays were reliable, specific, and rapid methods for the detection of porcine GCRV in porcine fecal samples.

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“…Human infection by GCRV has been associated with both sporadic episodes and large outbreaks of gastroenteritis in all age groups and appears to be globally distributed (1,2,5,6,18,24,25,34,38,41,43). Large-scale epidemiological studies have revealed that the prevalence may range from 0.6% to 6.8% (2,24), and GCRVs are regarded as emerging human pathogens.…”

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confidence: 99%

Detection and Characterization of Group C Rotaviruses in Asymptomatic Piglets in Ireland

2008

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Group C rotaviruses are important human enteric pathogens that have also been detected in a variety of mammalian species, including pigs. Group C rotaviruses have been identified in piglets with diarrhea, but their ecology remains to be elucidated. By screening of 292 fecal samples collected from 4-to 5-week-old asymptomatic pigs from four herds in Ireland between 2005 and 2007, 13 (4.4%) samples tested positive by reverse transcription-PCR for group C rotavirus. Group A rotaviruses were also detected in 19 samples but not in conjunction with group C viruses. The gene encoding the major group C neutralization antigen, the outer capsid protein VP7, was sequenced. The majority of the strains were very closely related to each other (>99% amino acid [aa] identity) and were characterized as genogroup G1 since they were genetically related to the prototype porcine strain Cowden (92.6% aa identity). Conversely, two strains (1GA/05/Cork/Ire and 281/07/ Dublin/Ire) were characterized as genogroup G6 since they displayed the highest identity (89.2 to 94.0% aa) to porcine G6 strains (43/06-22-like). Unexpectedly, one such G6 strain, 1GA/05/Cork/Ire, lacked the 4-aa insertion in the VP7 variable region VR8 found in all the other G6 group C rotaviruses. This study provides evidence that porcine group C rotavirus may be detected not infrequently in asymptomatic piglets. In addition, it provides evidence that, unlike the human viruses, porcine group C rotaviruses display broad genetic heterogeneity, which may pose a challenge for the development of prophylactic tools.

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Atypical human rotaviruses in Hungary

Cited by 10 publications

References 13 publications

Prevalence of Rotaviruses Groups A and C in Egyptian Children and Aquatic Environment

Prevalence of Rotaviruses Groups A and C in Egyptian Children and Aquatic Environment

Development of One-Step Real-Time Reverse Transcription Polymerase Chain Reaction Assays for Rapid Detection of Porcine Group C Rotaviruses

Detection and Characterization of Group C Rotaviruses in Asymptomatic Piglets in Ireland

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