Atypical epidemiology of CTX-M-15 among Enterobacteriaceae from a high diversity of non-clinical niches in Angola: Table 1.

Ribeiro, Teresa Gonçalves; Novais, Ângela; Peixe, Luı́sa; Machado, Elisabete

doi:10.1093/jac/dkv489

Cited by 29 publications

(39 citation statements)

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“…Presumptive qAmpC producers were preliminarily identified using phenotypic criteria and bla qAmpC genes were further identified by PCR and sequencing [8] . Isolates carrying bla qAmpC were also analysed for the presence of ESBL, carbapenemases or plasmid-mediated quinolone resistance genes [PMQR; qnr and aac(6’)-Ib-cr ] [2, 8, 11] . K. variicola , E. coli phylogroups, and E. coli O25b-ST131 were identified as described [10, 12] .…”

Section: Methodsmentioning

confidence: 99%

Significant temporal shifts on clonal and plasmid backgrounds of Enterobacteriaceae producing acquired AmpC in Portuguese clinical settings

Novais

Rodrigues

et al. 2019

Preprint

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Running title: Molecular epidemiology of qAmpC among clinical Enterobacteriaceae. 30 31 # These authors were equally contributed in this work. 32 33 34 Abbreviations 35 CS, conserved segment; ESBL, extended-spectrum β-lactamases; ICE, integrative and 36 conjugative element; icr-AmpC, inducible chromosomal AmpC; KL, capsular locus; MDR, 37 multidrug-resistant; MRR, multidrug resistance region; PMQR, plasmid-mediated quinolone 38 resistance; qAmpC, acquired AmpC β-lactamases; VR1, variable region 1. 39 ABSTRACT 40 OBJECTIVES: To provide detailed molecular data on clinical acquired AmpC (qAmpC)-41producing Enterobacteriaceae from two different periods (42 order to clarify the contribution of clonal and plasmid genetic platforms for the current 43 epidemiological scenario concerning extended-spectrum beta-lactams resistance. 44 METHODS:We analysed 1246 Enterobacteriaceae non-susceptible to third-generation 45 cephalosporins from 2 hospitals and 1 community laboratory between 2010 and 2013. 46Bacterial identification, antibiotic susceptibility, identification of qAmpC and plasmid-47 mediated quinolone resistance genes, clonal (PFGE, MLST) and plasmid (S1-/I-CeuI-PFGE, 48 replicon typing, hybridization) analysis were performed by standard methods. WGS was 49 performed in two ST11-K. pneumoniae isolates harbouring DHA-1. 50 RESULTS:The occurrence of qAmpC was lower (2.6%) than that observed in a previous 51 survey (7.4%), and varied slightly over time. Isolates produced DHA-1 (53%), CMY-2 (44%) 52 or DHA-6 (3%), but significant epidemiological changes were observed in the two surveys. 53While DHA-1 persisted in different institutions by selection of a worldwide epidemic IncR 54 plasmid in a ST11 harbouring KL105, CMY-2 rates increased over time linked to IncI1 55 plasmids (instead of IncK or IncA/C 2 ) in multiple E. coli clones. 56 CONCLUSIONS:The higher frequency of DHA-1 qAmpC in these species contrasts with 57 the scenario of most European countries. Furthermore, the different genetic backgrounds 58 associated with either ESBL or qAmpC in our country might have contributed to their 59 differential expansion. 60 61 IncR. 63 64 65 The worldwide contemporary epidemiology of Enterobacteriaceae resistant to broad-66 spectrum cephalosporins seems to be largely dominated by extended-spectrum β-lactamases 67 (ESBL) than acquired AmpC β-lactamases (qAmpC) [1]. However, the occurrence and 68 clinical impact of Enterobacteriaceae producing qAmpC is probably underestimated, since 69 qAmpC producers are difficult to identify by conventional methods and qAmpC expression is 70 frequently masked by other resistance mechanisms [2, 3]. Recent studies described an 71 increase of specific qAmpC enzymes among clinical Enterobacteriaceae [1, 2, 4], mostly 72 CMY-2-producing Escherichia coli associated either with hospital or community infections 73 and DHA-1-producing Klebsiella pneumoniae especially linked to nosocomial outbreaks [2, 74 3, 5, 6]. 75 76 In Portugal, relevant epidemiological changes regarding resistance to extended-spectr...

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Section: Methodsmentioning

confidence: 99%

Significant temporal shifts on clonal and plasmid backgrounds of Enterobacteriaceae producing acquired AmpC in Portuguese clinical settings

Novais

Rodrigues

et al. 2019

Preprint

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“…The authors also thank Dr Ruth M. Hall for kindly providing the rifampicinresistant mutant A. baumannii ATCC 17978 strain. [3]. e Untypeable by the current Acinetobacter replicon typing scheme [1].…”

Section: Acknowledgmentsmentioning

confidence: 99%

“…Curiously the OXA-23-producing A. towneri-like isolates were clonally unrelated, obtained from Lobito River and untreated wastewater (20 km distant). Moreover, these samples harboured additional resistant bacteria (Table 1) [3]. The OXA-58-producing A. johnsonii was obtained from treated tap water also positive for A. baumannii and A. pittii, suggesting an important role for water in the transmission of Acinetobacter spp.…”

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confidence: 97%

“…from nonclinical sources from Benguela Province (Angola) was evaluated as part of a larger surveillance project on bacterial resistance to clinically relevant antibiotics [3].…”

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confidence: 99%

“…From 63 samples [3] collected in different communes of Benguela Province, 29 revealed the presence of Acinetobacter, including rectal swabs from healthy volunteers (8/18), aquatic environments (river, human drinking water, wastewater; 8/20), rectal swabs from healthy animals (calf/cows; 3/10) and their respective environments (water, feed, floor/walls; 7/8) from four different farms, and drinking water from a wild park (3/3) (Supplementary Table S1). The remaining samples (rectal swabs from monkeys and goats) were negative for Acinetobacter.…”

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Exploring non-hospital-related settings in Angola reveals new Acinetobacter reservoirs for blaOXA-23 and blaOXA-58

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Grosso

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et al. 2016

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Antimicrobial use and resistance in food animal production: food safety and associated concerns in Sub-Saharan Africa

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et al. 2023

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The use of antimicrobials in food animal (FA) production is a common practice all over the world, with even greater usage and dependence in the developing world, including Sub-Saharan Africa (SSA). However, this practice which serves obvious economic benefits to producers has raised public health concerns over the last decades, thus driving the selection and dissemination of antimicrobial resistance and adversely impacting food safety and environmental health. This review presents the current and comprehensive antimicrobial usage practices in food animal production across SSA. We further highlighted the overall regional drivers as well as the public health, environmental, and economic impact of antimicrobial use in the production of food animals. Antimicrobial use is likely to increase with even exacerbated outcomes unless cost-effective, safe, and sustainable alternatives to antibiotics, especially probiotics, prebiotics, bacteriocins, antimicrobial peptides, bacteriophages, vaccines, etc. are urgently advocated for and used in food animal production in SSA. These, in addition to the implementation of strong legislation on antimicrobial use, and improved hygiene will help mitigate the public health concerns associated with antimicrobial use in food animals and improve the well-being and safety of food animals and their products.

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Atypical epidemiology of CTX-M-15 among Enterobacteriaceae from a high diversity of non-clinical niches in Angola: Table 1.

Cited by 29 publications

References 21 publications

Significant temporal shifts on clonal and plasmid backgrounds of Enterobacteriaceae producing acquired AmpC in Portuguese clinical settings

Significant temporal shifts on clonal and plasmid backgrounds of Enterobacteriaceae producing acquired AmpC in Portuguese clinical settings

Exploring non-hospital-related settings in Angola reveals new Acinetobacter reservoirs for blaOXA-23 and blaOXA-58

Antimicrobial use and resistance in food animal production: food safety and associated concerns in Sub-Saharan Africa

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