Atypical Decondensation of the Sperm Nucleus, Delayed Replication of the Male Genome and Sex Chromosome Positioning Following Intracytoplasmic Human Sperm Injection into Golden Hamster Eggs: Does ICSI Itself Introduce Chromosomal Anomalies?

Terada, Yayoi; Luetjens, C. Marc; Šutovský, Peter; Murakami, Taishi; Okamura, Kunihiro; Schatten, Gerald

doi:10.1016/s0015-0282(00)00904-3

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“…Hence, it is possible that in our experiments the onset of DNA replication was delayed too and started much later after the oocyte passed the critical period. After the injection of human spermatozoa into hamster oocytes Terada et al (2000) observed some oocytes in which the maternal pronuclei started to replicate DNA even in the presence of decondensing sperm heads which showed no BrdU incorporation. With prolonged culture and advanced sperm head decondensation replication was detected in almost all (maternal, paternal) pronuclei.…”

Section: Discussionmentioning

confidence: 98%

The absence of a DNA replication checkpoint in porcine zygotes

Vacková

Kren

Loi

et al. 2006

Zygote

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It has been demonstrated that in the zygotes of some mammals a unique checkpoint controls the onset of DNA replication. Thus, DNA replication begins in the maternal pronucleus only after the paternal pronucleus is fully formed. In our experiments we have investigated whether this checkpoint also operates in porcine zygotes produced either by in vitro fertilization (IVF) or by intracytoplasmic sperm injection (ICSI). Our results show that the onset of DNA replication occurs in the maternal pronucleus even in the presence of an intact sperm head in zygotes produced by ICSI, as well as in polyspermic eggs where some sperm heads are intact or male pronuclei are not yet fully developed. We conclude that in porcine zygotes there is an absence of the DNA replication checkpoint that is typical for some other mammals.

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Section: Discussionmentioning

confidence: 98%