Attraction of a synthetic aggregation pheromone of the brown-winged green bug, Plautia crossota stali Scott to its parasitoids, Gymnosoma rotundata and Trissolcus plautiae.

Mishiro, Koji; Ohira, Yoshio

doi:10.4241/kyubyochu.48.76

Cited by 10 publications

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“…Synthetic aggregation pheromones are currently used in pheromone-baited traps to survey orchard infestations by this insect (Toyama et al, 2015). The only way to control P. stali is to apply insecticides (Moriya & Shiga, 1984;Mishiro & Ohira, 2002). Therefore, once P. stali is found around an orchard, frequent application of large amounts of insecticide is required to prevent infestation.…”

Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%

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Identification and characterization of odorant‐binding protein genes in the brown‐winged green bug, Plautia stali

Taniai

Suetsugu

Miyazawa

2023

Entomologia Exp Applicata

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The brown‐winged green bug, Plautia stali Scott (Hemiptera: Pentatomidae), is a major pest in orchards in Japan and Korea. Large numbers of adults are attracted by aggregation pheromone [methyl (E,E,Z)‐2,4,6‐decatrienoate] and cause extensive damage to the fruit. To identify olfactory‐related genes in P. stali, we performed RNA sequencing (RNA‐seq) of adult antennae. Among the 27299 contigs, 16 putative odorant‐binding protein (OBP) genes were identified and designated as PstaOBP1–16, including 10 classic (i.e., containing six conserved cysteines) and six Plus‐C (i.e., containing more than six cysteines) OBP genes. Quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR) revealed that four genes (PstaOBP1, 3, 6, and 7) were strongly expressed in the antennae. PstaOBP2 was detected throughout the insect body, with higher expression in the antennae, stylet, and tarsus. The remaining OBP genes were expressed in very small amounts. PstaOBP1, which showed the highest expression in adult antennae, was expressed in Escherichia coli (Migula) Castellani & Chalmers cells and the recombinant protein was subjected to a ligand‐binding competitive assay. PstaOBP1 bound to methyl (E,E,Z)‐2,4,6‐decatrienoate with high affinity. Of the other 26 compounds tested, hinokitiol and tropolone bound strongly to PstaOBP1. Hinokitiol is an odor component of hinoki cypress, a host tree of P. stali. Electrophysiological responses to methyl (E,E,Z)‐2,4,6‐decatrienoate and hinokitiol were detected using adult P. stali antennae. Combined, these results suggest that PstaOBP1 is involved in P. stali responses to pheromone and host plant volatiles.

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Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%

“…The only way to control P . stali is to apply insecticides (Moriya & Shiga, 1984; Mishiro & Ohira, 2002). Therefore, once P .…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of odorant‐binding protein genes in the brown‐winged green bug, Plautia stali

Taniai

Suetsugu

Miyazawa

2023

Entomologia Exp Applicata

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Chemical Ecology of Tachinid Parasitoids

Nakamura

Ichiki

Kainoh

2013

Chemical Ecology of Insect Parasitoids

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Morphology and distribution of antennal sensilla in a parasitoid fly, Gymnosoma rotundatum (Diptera: Tachinidae)

Roh

Lee

Park

2020

Microscopy Res & Technique

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Morphology of antennal sensilla and their distribution were investigated in male and female adults of the parasitoid fly Gymnosoma rotundatum (Diptera: Tachinidae) using scanning electron microscopy (SEM). The overall length and shape were not different between males and females from each other. Three basic types of sensilla (sensilla basiconica, s. chaetica, and s. coeloconica) were identified from both sexes, but with variations in numbers and distribution along the antennae. The s. basiconica and s. chaetica could be divided further into subtypes; s. basiconica into three subtypes and s. chaetica into two subtypes. All the basiconica subtypes 1, 2, and 3 were multiporous, indicating that their primary function was olfactory. The sensilla basiconica was most abundant on the antennae of both sexes. The abundance of s. basiconica subtype 1 was different, but other subtypes 2 and 3 were similar between males and females. There was no pore on the cuticular surface of the s. chaetica and s. coeloconica, suggesting that they are likely to be a mechanosensory or a thermohygroreceptory function. The abundance of the two sensillum types was similar between males and females. The morphological information obtained in our study provides a basis for future investigations into the sensory physiological function, and associated behaviors, of each type of sensilla in this parasitoid fly. K E Y W O R D Santennae, morphology, scanning electron microscopy, sensilla

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Attraction of a synthetic aggregation pheromone of the brown-winged green bug, Plautia crossota stali Scott to its parasitoids, Gymnosoma rotundata and Trissolcus plautiae.

Cited by 10 publications

References 0 publications

Identification and characterization of odorant‐binding protein genes in the brown‐winged green bug, Plautia stali

Identification and characterization of odorant‐binding protein genes in the brown‐winged green bug, Plautia stali

Chemical Ecology of Tachinid Parasitoids

Morphology and distribution of antennal sensilla in a parasitoid fly, Gymnosoma rotundatum (Diptera: Tachinidae)

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