Attracted or Repelled?—A Matter of Two Neurons, One Pheromone Binding Protein, and a Chiral Center

Wojtasek, Hubert; Hansson, Bill S.; Leal, Walter S.

doi:10.1006/bbrc.1998.9278

Cited by 103 publications

(70 citation statements)

References 25 publications

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“…It was suggested that such amazing feats depend on ORNs being located within the same sensillum (Fadamiro et al, 1999), which is common among pheromone agonist/antagonist neurons in Lepidoptera Larsson et al, 2002). Similar arrangements are found also in Coleoptera (Wojtasek et al, 1998), but, so far, not in bark beetle pheromone neurons (Andersson et al, 2009;Mustaparta et al, 1977;Mustaparta et al, 1980). Consistent with behavioral observations, asynchronous arrival of pheromone constituents disrupts the spiking pattern of projection neurons in the antennal lobe, with resulting effects on the temporal aspects of odor coding (Christensen and Hildebrand, 1997).…”

Section: Discussionsupporting

confidence: 78%

“…Thus, neurons responding to compounds that together constitute an ecologically important signal should then often be found paired within the same sensillum. Good examples are the pheromone ORNs that are colocalized with ORNs that respond to pheromone antagonists Fadamiro et al, 1999;Larsson et al, 2002;Wojtasek et al, 1998). ORN cocompartmentalization might also provide the means for signal modulation in the periphery, in that responses in neighboring neurons could potentially affect the activity of each other (Getz and Akers, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Peripheral modulation of pheromone response by inhibitory host compound in a beetle

Andersson

Larsson

Blaženec

et al. 2010

Journal of Experimental Biology

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SUMMARYWe identified several compounds, by gas chromatographic-electroantennographic detection (GC-EAD), that were antennally active in the bark beetle Ips typographus and also abundant in beetle-attacked spruce trees. One of them, 1,8-cineole (Ci), strongly inhibited the attraction to pheromone in the field. Single-sensillum recordings (SSRs) previously showed olfactory receptor neurons (ORNs) on I. typographus antennae selectively responding to Ci. All Ci neurons were found within sensilla co-inhabited by a pheromone neuron responding to cis-verbenol (cV); however, in other sensilla, the cV neuron was paired with a neuron not responding to any test odorant. We hypothesized that the colocalization of ORNs had a functional and ecological relevance. We show by SSR that Ci inhibited spontaneous activity of the cV neuron only in sensilla in which the Ci neuron was also present. Using mixtures of cV and Ci, we further show that responses to low doses (1-10ng) of cV were significantly reduced when the colocalized Ci neuron simultaneously responded to high doses (1-10mg) of Ci. This indicated that the response of the Ci neuron, rather than ligand-receptor interactions in the cV neuron, caused the inhibition. Moreover, cV neurons paired with Ci neurons were more sensitive to cV alone than the ones paired with the non-responding ORN. Our observations question the traditional view that ORNs within a sensillum function as independent units. The colocalization of ORNs might sharpen adaptive responses to blends of semiochemicals with different ecological significance in the olfactory landscape.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Peripheral modulation of pheromone response by inhibitory host compound in a beetle

Andersson

Larsson

Blaženec

et al. 2010

Journal of Experimental Biology

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“…3). The ABPX subfamily also includes three beetle OBPs, the queen pheromone-binding protein from the honey bee Apis mellifera, and an OBP from the "true bug" Lygus lineolaris (Wojtasek et al 1998(Wojtasek et al , 1999Danty et al 1999;Vogt et al 1999). We have named another OBP subfamily the CRLBP family (Fig.…”

Section: Drosophila Obps Within the Insect Obp Familymentioning

confidence: 99%

“…The ABPX subfamily includes three Drosophila OBPs (Obp83a, Obp83b, and Obp69a) as well as antennal binding proteins termed ABPXs from a variety of Lepidoptera species (Krieger et al 1996(Krieger et al , 1997, and related antennal proteins from multiple species of beetle (Wojtasek et al 1998(Wojtasek et al , 1999, the honey bee A. mellifera (Danty et al 1999), and the Hemiptera ("true bug") L. lineolaris (Vogt et al 1999). Insect OBP progenitors were likely present in ancient Neoptera, and the ABPX subfamily evidently diverged from other insect OBPs before the Endopterygota-Paraneoptera split.…”

Section: Phylogenetic Analysis Of the Insect Obp Familymentioning

confidence: 99%

Genome-Wide Analysis of the Odorant-Binding Protein Gene Family in Drosophila melanogaster

Hekmat-Scafe¹,

Scafe²,

McKinney³

et al. 2002

Genome Res.

411

404

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Olfaction is of considerable importance to many insects in behaviors critical for survival and reproduction, including location of food sources, selection of mates, recognition of colony con-specifics, and determination of oviposition sites. An ubiquitous, but poorly understood, component of the insect's olfactory system is a group of odorant-binding proteins (OBPs) that are present at high concentrations in the aqueous lymph surrounding the dendrites of olfactory receptor neurons. OBPs are believed to shuttle odorants from the environment to the underlying odorant receptors, for which they could potentially serve as odorant presenters. Here we show that the Drosophila genome carries 51 potential OBP genes, a number comparable to that of its odorant-receptor genes. We find that the majority (73%) of these OBP-like genes occur in clusters of as many as nine genes, in contrast to what has been observed for the Drosophila odorant-receptor genes. Two of the presumptive OBP gene clusters each carries an odorant-receptor gene. We also report an intriguing subfamily of 12 putative OBPs that share a unique C-terminal structure with three conserved cysteines and a conserved proline. Members of this subfamily have not previously been described for any insect. We have performed phylogenetic analyses of the OBP-related proteins in Drosophila as well as other insects, and we discuss the duplication and divergence of the genes for this large family.

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“…Odorants that pass through pores on the sensillum bind to ORs expressed on the dendrite of OSNs and induce an action potential, which can be monitored using the single sensillum recording (SSR) technique (Bestmann et al, 1996;Stensmyr et al, 2003;Wojtasek et al, 1998); a recording electrode is placed in the desired sensillum and captures voltage changes due to the firing of the OSNs ( Fig. 2A).…”

Section: Functional Characterization Of Insect Orsmentioning

confidence: 99%

Smelling the difference: controversial ideas in insect olfaction

Pellegrino

Nakagawa

2009

Journal of Experimental Biology

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SummaryIn animals, the sense of smell is often used as a powerful way to attract potential mates, to find food and to explore the environment. Different animals evolved different systems to detect volatile odorants, tuned to the specific needs of each species. Vertebrates and nematodes have been used extensively as models to study the mechanisms of olfaction: the molecular players are olfactory receptors (ORs) expressed in olfactory sensory neurons (OSNs) where they bind to volatile chemicals, acting as the first relay of olfactory processing. These receptors belong to the G protein-coupled receptor (GPCR) superfamily; binding to odorants induces the production and amplification of second messengers, which lead to the depolarization of the neuron. The anatomical features of the insect olfactory circuit are similar to those of mammals, and until recently it was thought that this similarity extended to the ORs, which were originally annotated as GPCRs. Surprisingly, recent evidence shows that insect ORs can act like ligand-gated ion channels, either completely or partially bypassing the amplification steps connected to the activation of G proteins. Although the involvement of G proteins in insect olfactory signal transduction is still under question, this new discovery raises fascinating new questions regarding the function of the sense of smell in insects, its evolution and potential benefits compared with its mammalian counterpart.

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Attracted or Repelled?—A Matter of Two Neurons, One Pheromone Binding Protein, and a Chiral Center

Cited by 103 publications

References 25 publications

Peripheral modulation of pheromone response by inhibitory host compound in a beetle

Peripheral modulation of pheromone response by inhibitory host compound in a beetle

Genome-Wide Analysis of the Odorant-Binding Protein Gene Family in Drosophila melanogaster

Smelling the difference: controversial ideas in insect olfaction

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