Attomole detection of proteins by matrix‐assisted laser desorption/ionization mass spectrometry with the use of picolitre vials

Jespersen, Sonja; Niessen, W.M.A.; Tjaden, U.R.; Greef, J. van der; Litborn, Erik; Lindberg, Ulf; Roeraade, Johan; Hillenkamp, Franz

doi:10.1002/rcm.1290080802

Cited by 101 publications

(73 citation statements)

References 12 publications

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“…Nevertheless, the material deposited in each spot can be used to obtain dozens of spectra, each an average of 500 individual shots. Our detection limits, single picomoles per spot and tens to hundreds of femtomoles per spectrum, do not approach the attomole level attained through picoliter sample preparations (35), but are competitive with Coomassie staining (about 10 pmol) or silver staining (about 100 fmol) for proteins of this size (36).…”

Section: Resultsmentioning

confidence: 97%

Observation ofEscherichia coliRibosomal Proteins and Their Posttranslational Modifications by Mass Spectrometry

Arnold

Reilly

1999

Analytical Biochemistry

202

217

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Section: Resultsmentioning

confidence: 97%

Observation ofEscherichia coliRibosomal Proteins and Their Posttranslational Modifications by Mass Spectrometry

Arnold

Reilly

1999

Analytical Biochemistry

202

217

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“…One approach minimizes dispersion of sample materials by etching or drilling small channels on the target [1,2] or using hydrophobic materials as MALDI plate surfaces [3][4][5][6]. A second approach uses microdispensing, including use of capillary tubes [7][8][9][10], piezoelectric techniques [11][12][13][14], electrodynamic methods [15], and acoustic ejection [16].…”

mentioning

confidence: 99%

Improving the signal intensity and sensitivity of MALDI mass spectrometry by using nanoliter spots deposited by induction-based fluidics

Sauter²,

Gross

2008

J. Am. Soc. Mass Spectrom.

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A new contact-free, small droplet deposition method using an induction-based fluidics (IBF) technique to dispense nanoliter drops is described and evaluated for sample preparation in matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). The signal intensities available when using nanoliter spots are greater than those obtained with normal, microliter spots when the same amount of analyte is used. When using an ionic-liquid matrix, the improvement in sensitivity is equal to the concentration enhancement that was achieved by using smaller quantities of matrix. When using a conventional solid matrix, however, the increase in signal intensity shows a more complicated relationship to concentration. The approach of nanoliter deposition also supports multiple spotting to increase sample concentration and, thus, sample signal intensity. Nanoliter spotting not only improves the signal intensity and sensitivity achieved by MALDI-MS but also allows a major fraction of trace samples to be saved for other experiments, thus expanding the application of MALDI-MS to biological studies where sample quantity is limited. A lthough matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has wide applicability to biological and synthetic polymers, obtaining high-quality MS signals when the quantity of sample is small is difficult-a challenge that is endemic in biological studies. A limited sample amount gives rise to poor signal intensity, hampering spectra interpretation, unknown identification, and quantification, thus limiting the application in biological studies. One way to increase the signal intensity without increasing the sample amount is to make MALDI spots smaller and more concentrated.Small-scale sample preparation on a MALDI target has been achieved in several ways. One approach minimizes dispersion of sample materials by etching or drilling small channels on the target [1, 2] or using hydrophobic materials as MALDI plate surfaces [3][4][5][6]. A second approach uses microdispensing, including use of capillary tubes [7][8][9][10], piezoelectric techniques [11][12][13][14], electrodynamic methods [15], and acoustic ejection [16]. This general approach improves MALDI for imaging [14,16], on-line analysis [13,17], and concentration enhancement by microextraction [10]. Common in both approaches [1-12, 15, 17] is the concept that the sensitivity of MALDI-MS increases when small sample spots are used and that the search for "hot spots" within the sample area becomes more productive when solid matrices are used. Our approach of using small volumes of concentrated analytes is motivated by the concept of purposefully creating a "hot spot."Herein, we describe an approach by which a nanoliter syringe and induction-based fluidics (IBF) deposit small-volume droplets (in the nanoliter range) in a noncontact, flight-controlled way. Using this technique, we first investigated the relationship between the spot size and signal intensity with an ionic-liquid matrix (ILM), taking advantage of the homo...

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“…4) (Jespersen et al, 1994;Ekström et al, 2000;Finnskog et al, 2006). A number of reports on fabrication of such microstructured MALDI plates with extended functionalities originates from Laurell and co-workers (Ekström et al, 2000(Ekström et al, , 2001aMarko-Varga et al, 2001;Finnskog et al, 2006).…”

Section: A Preconcentration and Sensitivity Enhancementmentioning

confidence: 96%

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

Urban

Amantonico

Zenobi

2011

Mass Spectrometry Reviews

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We review the literature that describes how (matrix-assisted) laser desorption/ionization (MA)LDI target plates can be used not only as sample supports, but beyond that: as functional parts of analytical protocols that incorporate detection by MALDI-MS or matrix-free LDI-MS. Numerous steps of analytical procedures can be performed directly on the (MA)LDI target plates prior to the ionization of analytes in the ion source of a mass spectrometer. These include homogenization, preconcentration, amplification, purification, extraction, digestion, derivatization, synthesis, separation, detection with complementary techniques, data storage, or other steps. Therefore, we consider it helpful to define the "lab-on-a-plate" as a format for carrying out extensive sample treatment as well as bioassays directly on (MA)LDI target plates. This review introduces the lab-on-plate approach and illustrates it with the aid of relevant examples from the scientific and patent literature.

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Attomole detection of proteins by matrix‐assisted laser desorption/ionization mass spectrometry with the use of picolitre vials

Cited by 101 publications

References 12 publications

Observation ofEscherichia coliRibosomal Proteins and Their Posttranslational Modifications by Mass Spectrometry

Observation ofEscherichia coliRibosomal Proteins and Their Posttranslational Modifications by Mass Spectrometry

Improving the signal intensity and sensitivity of MALDI mass spectrometry by using nanoliter spots deposited by induction-based fluidics

Lab‐on‐a‐plate: Extending the functionality of MALDI‐MS and LDI‐MS targets

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