Attomolar Detection of a Cancer Biomarker Protein in Serum by Surface Plasmon Resonance Using Superparamagnetic Particle Labels

Krishnan, Sadagopan; Mani, Vigneshwaran; Wasalathanthri, Dhanuka P.; Kumar, Challa V.; Rusling, James F.

doi:10.1002/anie.201005607

Cited by 185 publications

(152 citation statements)

References 29 publications

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“…iron oxide-poly(styrene) beads greatly amplify SPR signals by increasing the refractive index in the detection window of the SPR sensor. 23 MP-Ab 2 beads with captured IgEs were washed, separated magnetically, then redispersed and injected into the flow system to deliver them to the gold SPRi chip, where SPR signals for spots are imaged simultaneously. Capture on magnetic beads facilitates separation of IgEs from the complex serum mixture.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive carbohydrate-peptide SPR imaging microarray for diagnosing IgE mediated peanut allergy

Joshi

Peczuh

Kumar

et al. 2014

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Severity of peanut allergies is linked to allergen-specific immunoglobulin E (IgE) antibodies in blood, but diagnostics from assays using glycoprotein allergen mixtures may be inaccurate. Measuring IgEs specific to individual peptide and carbohydrate epitopes of allergenic proteins is promising. We report here the first immunoarray for IgEs utilizing both peptide and carbohydrate epitopes. A surface plasmon resonance imaging (SPRi) microarray was equipped with peptide and β-xylosyl glycoside (BXG) epitopes from major peanut allergen glycoprotein Arachis hypogaea h2 (Ara-h2). A monoclonal anti-IgE antibody was included as positive control. IgEs were precaptured onto magnetic beads loaded with polyclonal anti-IgE antibodies to enhance sensitivity and minimize non-specific binding. As little as 0.1 attomole (0.5 pg/mL) IgE was detected from dilute serum in 45 min. IgEs binding to Ara-h2 peptide and BXG were quantified in 10 μL of patient serum and correlated with standard ImmunoCAP values.

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Section: Introductionmentioning

confidence: 99%

Ultrasensitive carbohydrate-peptide SPR imaging microarray for diagnosing IgE mediated peanut allergy

Joshi

Peczuh

Kumar

et al. 2014

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“…For VEGF-C detection, monoclonal primary antihuman VEGF-C antibodies were covalently immobilized onto carboxylate-functionalized AuNPs [76]. After VEGF-C capture, AuNP-antibody (AuNPmAb) and AuNP-antibody-VEGF-C particles coexisted in a dispersion.…”

Section: (C) Proteinsmentioning

confidence: 99%

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Wang

Mirkin

2017

Proc. R. Soc. A.

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Along with more prevalent solid-state nanopores, glass or quartz nanopipettes have found applications in resistive-pulse and rectification sensing. Their advantages include the ease of fabrication, small physical size and needle-like geometry, rendering them useful for local measurements in small spaces and delivery of nanoparticles/biomolecules. Carbon nanopipettes fabricated by depositing a thin carbon layer on the inner wall of a quartz pipette provide additional means for detecting electroactive species and fine-tuning the current rectification properties. In this paper, we discuss the fundamentals of resistivepulse sensing with nanopipettes and our recent studies of current rectification in carbon pipettes.

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“…Measurements of PSA in cell lysates and human serum of cancer patients gave excellent correlations with the standard ELISA assays. In another work, the clustering of MB labels on the SPR sensor surface led to unprecedented sensitivity and ultralow DL for PSA in serum (Krishnan et al, 2011). To fabricate the sensor, Ab 1 was covalently immobilized onto carboxylatefunctionalized Au-SPR chips, while SPR was monitored continuously after the injection of the MBAb 2 -PSA bioconjugates.…”

Section: Magnetic Nanomaterialsmentioning

confidence: 99%

Detection of Cancer Biomarkers With Nanotechnology

Zhang

et al. 2013

American Journal of Biochemistry and Biotechnology

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Early detection of cancer biomarkers with high precision is critically important for cancer therapy. A variety of sensors based on different nanostructured materials have attracted intensive research interest due to their potential for highly sensitive and selective detection of cancer biomarkers. This review covers the use of a variety of nanostructured materials, including carbon nanotubes, silicon nanowires, gold nanoparticles and quantum dots, in the fabrication of sensors. Emphases are placed on how the detection systems work and what detection limits can be achieved. Some assays described in this review outperform established methods for cancer biomarker detection. It is highly promising that these sensors would soon move into commercial-scale production and find routine use in hospitals.

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Attomolar Detection of a Cancer Biomarker Protein in Serum by Surface Plasmon Resonance Using Superparamagnetic Particle Labels

Cited by 185 publications

References 29 publications

Ultrasensitive carbohydrate-peptide SPR imaging microarray for diagnosing IgE mediated peanut allergy

Ultrasensitive carbohydrate-peptide SPR imaging microarray for diagnosing IgE mediated peanut allergy

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Detection of Cancer Biomarkers With Nanotechnology

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